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| Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
19/09/2018 |
Data da última atualização: |
19/09/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LEDO, A. da S.; JENDEREK, M. M.; LEDO, C. A. da S.; AYALA-SILVA, T. |
Afiliação: |
ANA DA SILVA LEDO, CPATC; MARIA M. JENDEREK, USDA-ARS; CARLOS ALBERTO DA SILVA LEDO, CNPMF; TOMAS AYALA-SILVA, USDA-ARS. |
Título: |
Antioxidants and phenolic secretion in sugarcane genotypes shoots culture. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Journal of Agricultural Science, v. 10, n. 5, 2018. |
ISSN: |
1916-9760 |
Idioma: |
Inglês |
Conteúdo: |
Secretion of phenolic compounds is a major limitation for sugarcane in vitro shoot culture, causing a loss of regenerative capacity and subsequent cell death. In this study, micropropagation and phenolic secretion of four Saccharum genotypes were evaluated in presence of different antioxidants. Aseptic cultures of S. officinarum (PI 184794 and PI 88652), S. sinense (PI 29109) and S. robustum (UNK R65P35) were propagated on medium containing antioxidants, citric acid (100 mg/L), L-cysteine (100 mg/L), polyvynylpirrolidone (300 mg/L) and L-glutathione (50 mg/L) in two consecutive subculture cycles. Interaction between genotypes and antioxidants was significant in both cycles. All genotypes showed good shoot formation, shoot vigor and color, except in PI 88652 which had less shoot development in both the presence and absence of the antioxidants tested. PI 184794 displayed the highest shoot proliferation in the presence of citric acid, and UNK R65P35 produced more shoots per explant in the 2nd subculture. For S. sinense (PI 29109), in both subcultures, most shoots were observed in the presence of polyvynylpirrolidone. Medium discoloration due to phenolic secretion was reduced in the presence of citric acid and polyvynylpirrolidone. The type of secreted phenolic compounds differed with genotype as the Principal Component Analysis of cultivation media separated PI 88652 from PI 29109 and UKN R65P35. Phenolic compounds varied in composition and were secreted at various levels as a function of genotype and antioxidant type. Loadings plots indicated the genotype and antioxidant separations were broadly driven by flavonoid compounds. MenosSecretion of phenolic compounds is a major limitation for sugarcane in vitro shoot culture, causing a loss of regenerative capacity and subsequent cell death. In this study, micropropagation and phenolic secretion of four Saccharum genotypes were evaluated in presence of different antioxidants. Aseptic cultures of S. officinarum (PI 184794 and PI 88652), S. sinense (PI 29109) and S. robustum (UNK R65P35) were propagated on medium containing antioxidants, citric acid (100 mg/L), L-cysteine (100 mg/L), polyvynylpirrolidone (300 mg/L) and L-glutathione (50 mg/L) in two consecutive subculture cycles. Interaction between genotypes and antioxidants was significant in both cycles. All genotypes showed good shoot formation, shoot vigor and color, except in PI 88652 which had less shoot development in both the presence and absence of the antioxidants tested. PI 184794 displayed the highest shoot proliferation in the presence of citric acid, and UNK R65P35 produced more shoots per explant in the 2nd subculture. For S. sinense (PI 29109), in both subcultures, most shoots were observed in the presence of polyvynylpirrolidone. Medium discoloration due to phenolic secretion was reduced in the presence of citric acid and polyvynylpirrolidone. The type of secreted phenolic compounds differed with genotype as the Principal Component Analysis of cultivation media separated PI 88652 from PI 29109 and UKN R65P35. Phenolic compounds varied in composition and were secreted at various levels as a ... Mostrar Tudo |
Thesagro: |
Cana de Açúcar. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02181naa a2200181 a 4500 001 2096031 005 2018-09-19 008 2018 bl uuuu u00u1 u #d 022 $a1916-9760 100 1 $aLEDO, A. da S. 245 $aAntioxidants and phenolic secretion in sugarcane genotypes shoots culture.$h[electronic resource] 260 $c2018 520 $aSecretion of phenolic compounds is a major limitation for sugarcane in vitro shoot culture, causing a loss of regenerative capacity and subsequent cell death. In this study, micropropagation and phenolic secretion of four Saccharum genotypes were evaluated in presence of different antioxidants. Aseptic cultures of S. officinarum (PI 184794 and PI 88652), S. sinense (PI 29109) and S. robustum (UNK R65P35) were propagated on medium containing antioxidants, citric acid (100 mg/L), L-cysteine (100 mg/L), polyvynylpirrolidone (300 mg/L) and L-glutathione (50 mg/L) in two consecutive subculture cycles. Interaction between genotypes and antioxidants was significant in both cycles. All genotypes showed good shoot formation, shoot vigor and color, except in PI 88652 which had less shoot development in both the presence and absence of the antioxidants tested. PI 184794 displayed the highest shoot proliferation in the presence of citric acid, and UNK R65P35 produced more shoots per explant in the 2nd subculture. For S. sinense (PI 29109), in both subcultures, most shoots were observed in the presence of polyvynylpirrolidone. Medium discoloration due to phenolic secretion was reduced in the presence of citric acid and polyvynylpirrolidone. The type of secreted phenolic compounds differed with genotype as the Principal Component Analysis of cultivation media separated PI 88652 from PI 29109 and UKN R65P35. Phenolic compounds varied in composition and were secreted at various levels as a function of genotype and antioxidant type. Loadings plots indicated the genotype and antioxidant separations were broadly driven by flavonoid compounds. 650 $aCana de Açúcar 700 1 $aJENDEREK, M. M. 700 1 $aLEDO, C. A. da S. 700 1 $aAYALA-SILVA, T. 773 $tJournal of Agricultural Science$gv. 10, n. 5, 2018.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
15/09/1999 |
Data da última atualização: |
15/09/1999 |
Autoria: |
ARAUJO, M. T.; BUSO, J. A.; REIS, N. V. |
Afiliação: |
EMBRAPA-CNPH, Brasilia, DF. |
Título: |
Efeito de epocas de plantio de bulbos vernalizados na producao de sementes de cebola. |
Ano de publicação: |
1983 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE OLERICULTURA, 23., 1983, Rio de Janeiro, RJ. Resumos... Rio de Janeiro: SOB, 1983. |
Páginas: |
p.109. |
Idioma: |
Português |
Palavras-Chave: |
Brasil; Brasilia; Distrito Federal; Onion; Production. |
Thesagro: |
Allium Cepa; Bulbo; Cebola; Cerrado; Época de Plantio; Produção; Semente; Vernalização. |
Thesaurus NAL: |
Brazil; bulbs; planting date; seeds; vernalization. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00951naa a2200361 a 4500 001 1765489 005 1999-09-15 008 1983 bl uuuu u00u1 u #d 100 1 $aARAUJO, M. T. 245 $aEfeito de epocas de plantio de bulbos vernalizados na producao de sementes de cebola. 260 $c1983 300 $ap.109. 650 $aBrazil 650 $abulbs 650 $aplanting date 650 $aseeds 650 $avernalization 650 $aAllium Cepa 650 $aBulbo 650 $aCebola 650 $aCerrado 650 $aÉpoca de Plantio 650 $aProdução 650 $aSemente 650 $aVernalização 653 $aBrasil 653 $aBrasilia 653 $aDistrito Federal 653 $aOnion 653 $aProduction 700 1 $aBUSO, J. A. 700 1 $aREIS, N. V. 773 $tIn: CONGRESSO BRASILEIRO DE OLERICULTURA, 23., 1983, Rio de Janeiro, RJ. Resumos... Rio de Janeiro: SOB, 1983.
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