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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
27/01/2021 |
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Data da última atualização: |
27/01/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUTO, B. de M.; ARAÚJO, A. C. B. de; HAMANN, P. R. V.; BASTOS, A. de R.; CUNHA, I. de S.; PEIXOTO, J.; KRUGER, R. H.; NORONHA, E. F.; QUIRINO, B. F. |
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Afiliação: |
BETULIA DE MORAIS SOUTO, CNPAE; Ana Carolina Bitencourt de Araújo; Pedro Ricardo Vieira Hamann, Universidade de Brasília; Andrêssa de Rezende Bastos; Isabel de Souza Cunha, Universidade Católica de Brasília; Julianna Peixoto, Universidade de Brasília; Ricardo Henrique Kruger, Universidade de Brasília; Eliane Ferreira Noronha, Universidade de Brasília; BETANIA FERRAZ QUIRINO, CNPAE. |
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Título: |
Functional screening of a Caatinga goat (Capra hircus) rumen metagenomic library reveals a novel GH3 beta-xylosidase. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
PLoS ONE, v. 16, n. 1, e024511, 2021. |
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DOI: |
https://doi.org/10.1371/journal.pone.0245118 |
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Idioma: |
Inglês |
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Conteúdo: |
Functional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-beta-D-glucopyranoside (pNPG), 4-nitrophenyl- beta -D-xylopyranoside (pNPX) and 4-nitrophenyl- beta -D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with beta-glucosidase, beta-xylosidase, and cellobiohydrolase activities. However, further testing with five natural substrates revealed that, although BGL11 has multiple substrate specificity, it is most active towards xylobiose. Thus, in its native goat rumen environment, BGL11 most likely functions as an extracellular beta-xylosidase acting on hemicellulose. Biochemical characterization of BGL11 showed an optimal pH of 5.6, and an optimal temperature of 50°C. Enzyme stability, an important parameter for industrial application, was also investigated. At 40°C purified BGL11 remained active for more than 15 hours without reduction in activity, and at 50°C, after 7 hours of incubation, BGL11 remained 60% active. In contrast to BLG11, most beta-xylosidases kinetically studied belong to the GH43 family and have been characterized only using synthetic substrates. In industry, beta-xylosidases can be used for plant biomass deconstruction, and the released sugars can be fermented into valuable bio-products, ranging from the biofuel ethanol to the sugar substitute xylitol. MenosFunctional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-beta-D-glucopyranoside (pNPG), 4-nitrophenyl- beta -D-xylopyranoside (pNPX) and 4-nitrophenyl- beta -D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with beta-glucosidase, beta-xylosidase, and cellobiohydrolase activities. However, further testing with five natural substrates revealed that, although BGL11 has multiple substrate specificity, it is most active towards xylobiose. Thus, in its native goat rumen environment, BGL11 most likely functions as an extracellular beta-xylosidase acting on hemicellulose. Biochemical characterization of BGL11 showed an optimal pH of 5.6, and an optimal temperature of 50°C. Enzyme stability, an important parameter for industrial application, was also investigated. At 40°C purified BGL11 remained active for more than 15 hours without reduction in activity, and at 50°C, after 7 hours of incubation, BGL11 remained 60% active. In contrast to BLG11, most beta-xylosidases kinetical... Mostrar Tudo |
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Thesagro: |
Clonagem; Enzima; Peptídeo; Programa de Computador. |
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Thesaurus Nal: |
Cloning (animals); Computer software; Enzymes; Metagenomics; Prevotella; Screening; Signal peptide. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/220708/1/Functional-screening-of-a-Caatinga-goat-2021.pdf
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Marc: |
LEADER 02852naa a2200361 a 4500
001 2129624
005 2021-01-27
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1371/journal.pone.0245118$2DOI
100 1 $aSOUTO, B. de M.
245 $aFunctional screening of a Caatinga goat (Capra hircus) rumen metagenomic library reveals a novel GH3 beta-xylosidase.$h[electronic resource]
260 $c2021
520 $aFunctional screening of metagenomic libraries is an effective approach for identification of novel enzymes. A Caatinga biome goat rumen metagenomic library was screened using esculin as a substrate, and a gene from an unknown bacterium encoding a novel GH3 enzyme, BGL11, was identified. None of the BGL11 closely related genes have been previously characterized. Recombinant BGL11 was obtained and kinetically characterized. Substrate specificity of the purified protein was assessed using seven synthetic aryl substrates. Activity towards nitrophenyl-beta-D-glucopyranoside (pNPG), 4-nitrophenyl- beta -D-xylopyranoside (pNPX) and 4-nitrophenyl- beta -D-cellobioside (pNPC) suggested that BGL11 is a multifunctional enzyme with beta-glucosidase, beta-xylosidase, and cellobiohydrolase activities. However, further testing with five natural substrates revealed that, although BGL11 has multiple substrate specificity, it is most active towards xylobiose. Thus, in its native goat rumen environment, BGL11 most likely functions as an extracellular beta-xylosidase acting on hemicellulose. Biochemical characterization of BGL11 showed an optimal pH of 5.6, and an optimal temperature of 50°C. Enzyme stability, an important parameter for industrial application, was also investigated. At 40°C purified BGL11 remained active for more than 15 hours without reduction in activity, and at 50°C, after 7 hours of incubation, BGL11 remained 60% active. In contrast to BLG11, most beta-xylosidases kinetically studied belong to the GH43 family and have been characterized only using synthetic substrates. In industry, beta-xylosidases can be used for plant biomass deconstruction, and the released sugars can be fermented into valuable bio-products, ranging from the biofuel ethanol to the sugar substitute xylitol.
650 $aCloning (animals)
650 $aComputer software
650 $aEnzymes
650 $aMetagenomics
650 $aPrevotella
650 $aScreening
650 $aSignal peptide
650 $aClonagem
650 $aEnzima
650 $aPeptídeo
650 $aPrograma de Computador
700 1 $aARAÚJO, A. C. B. de
700 1 $aHAMANN, P. R. V.
700 1 $aBASTOS, A. de R.
700 1 $aCUNHA, I. de S.
700 1 $aPEIXOTO, J.
700 1 $aKRUGER, R. H.
700 1 $aNORONHA, E. F.
700 1 $aQUIRINO, B. F.
773 $tPLoS ONE$gv. 16, n. 1, e024511, 2021.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Biblioteca |
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| Registros recuperados : 64 | |
| 42. |  | QUIRINO, B. F.; BARRETO, C.; COSTA, O. Y.; SOUTO, B. de M.; TUPINAMBA, D.; BERGMANN, J. C.; KRUGER, R. H.; KYAW, C. Microbial diversity in different stages of the ethanol production process using culture-independent techniques. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 37., 2015, San Diego, California. [Resumos ...] Fairfax: Society for Industrial Microbiology and Biotechnology, 2015. Resumo T45| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 43. | | SILVA, M. R. S. S. da; BRESOLIN, J. D.; KRUGER, R. H.; BUSTAMANTE, M. M. da C.; REIS JÚNIOR, F. B. dos. Efeito do fogo sobre a comunidade bacteriana de solos de Cerrado. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 23., 2005, Santos, SP. Resumos. Santos: Sociedade Brasileira de Microbiologia, 2005. 1 CD-ROM.| Biblioteca(s): Embrapa Cerrados. |
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| 44. | | SILVA, M. R. S. S. da; BRESOLIN, J. D.; KRUGER, R. H.; BUSTAMANTE, M. M. C.; REIS JUNIOR, F. B. dos. Effects of land use changes on soil microbial communities in the Cerrado region. In: CONFERÊNCIA CIENTÍFICA DO LBA, 3., 2004, Brasília, DF. Anais de trabalhos completos. Brasília, DF: LBA, 2004. 1 p.| Biblioteca(s): Embrapa Cerrados. |
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| 45. | | ARAUJO, A. C. B.; CARVALHO, L. S.; TUPINAMBA, D. D.; ALMEIDA, J. M.; FAVARO, L. C. de L.; KRUGER, R. H.; QUIRINO, B. F. Isolamento e Caracterização de Microrganismos Isolados a Partir de Solo de Cerrado para a Produção de Etanol de Segunda Geração. In: CONGRESSO LATINO AMERICANO DE MICROBIOLOGIA, 21., 2012, Santos, SP. [Anais...]. São Paulo: Sociedade Brasileira de Microbiologia: Asociación Latinoamericana de Microbiologia, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 46. | | SOUTO, B. de M.; CARVALHO, L. S.; LOPIES, N. S. P. R.; CUNHA, I. S.; KRUGER, R. H.; QUIRINO, B. F. Identification of clones with Beta-Glucosidase and cellobiohydrolase activities from a goat rumen metagenomic library. In: BRAZILIAN BIOENERGY SCIENCE AND TECHNOLOGY CONFERENCE - BBEST, 1., 2011, Campos do Jordão, SP. [Anais...]. São Paulo: FAPESP, 2011. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 47. | | QUIRINO, B. F.; BERGMAN, J. C.; COSTA, O. Y. A.; PALUAN, S. G.; RAMOS, N. S. P. L.; SOUTO, B. de M.; BARRETO, C. C.; KRUGER, R. H. Identification of glycosyl hydrolases in the microbial biodiversity of Brazil using a metagenomic approach. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 34., 2012, New Orleans. [Anais...]. Fairfax, VA: Society for Industrial Microbiology & Biotechnology, 2012. Não paginado. Poster Session 1: 7-44.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 48. | | GUIMARÃES, H. I. P.; SANTANA, R. H.; SILVEIRA, R.; PINTO, O. H. B.; QUIRINO, B. F.; BARRETO, C. C.; BUSTAMANTE, M. M. da C.; KRÜGER, R. H. Seasonal Variations in Soil Microbiota Profile of Termite (Syntermes wheeleri) Mounds in the Brazilian Tropical Savanna. Microorganisms, v. 8, 1482, 2020. 8 Special Issue: Soil Microbiome: Biotic and Abiotic Interactions.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 49. | | CATÃO, E.; LOPES, F. A. C.; ARAÚJO, J. F.; CASTRO, A. C. de; BARRETO, C. C.; BUSTAMANTE, M.; QUIRINO, B. F.; KRUGER, R. H. Soil Acidobacterial 16S rRNA Gene Sequences Reveal Subgroup Level Differences between Savanna-Like Cerrado and Atlantic Forest Brazilian Biomes. International Journal of Microbiology, article 156341, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 50. | | BERGMANN, J. C.; COSTA, M. M. do C.; JUNIOR, P. G. J.; BARRETO, C. C.; KRUGER, R. H.; QUIRINO, B. F. Soil bacterial community structure associated with oil palm (Elaeis guineensis) fatal yellowing disease. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 36., 2014, Flórida. Abstracts... Fairfax: Society for Industrial Microbiology and Biotechnology, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 51. | | BERGMANN, J. C.; BOARI, A. de J.; KRUGER, R. H.; TAVARES P.; TOGAWA, R. C.; SILVA JUNIOR, O. B. da; PAPPAS JUNIOR, G. J.; QUIRINO, B. F. New strategies to look at old problems: fatal yellowing in palm oil tree. In: BRAZILIAN BIOENERGY SCIENCE AND TECHNOLOGY CONFERENCE - BBEST, 1., 2011, Campos do Jordão, SP. [Anais...]. São Paulo: FAPESP, 2011. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 52. | | BERGMANN, J. C.; BOARI, A. de J.; KRUGER, R. H.; TAVARES, P.; TOGAWA, R. C.; SILVA JUNIOR, O. B. da; PAPPAS JUNIOR, G. J.; QUIRINO, B. F. New strategies to look at old problems: fatal yellowing in palm oil tree. In: BRAZILIAN BIOENERGY SCIENCE AND TECHNOLOGY CONFERENCE - BBEST, 1., 2011, Campos do Jordão, SP. [Anais...]. São Paulo: FAPESP, 2011. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia; Embrapa Amazônia Oriental. |
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| 53. | | CUNHA, I. S.; FREIRE, M. S.; LEMOS, T. O.; BARRETO, C. C.; QUIRINO, B. F.; FRANCO, O. L.; KRUGER, R. H. Prokaryotic diversity present in the semi-arid goat rumen and its biotechnological potential. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR – SBBq, 39.; LATIN AMERICAN SYMPOSIUM ON THE MOLECULAR MECHANISMS OF SKELETAL MINERALIZATION, 1., 2010, Foz do Iguaçu, PR. Resumos... São Paulo: SBBq, 2010. Não paginado. Disponível em: < http://www.sbbq.org.br/2010/cdrom/busca.htm?query=quirino%2C+b.+f. >. Acesso em: 16 ago. 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 54. | | GOMES, P. H. M.; CÂNDIDO, E. S.; AQUINO, E. N.; NORONHA, E. F.; FRANCO, O. L.; FONTES, W.; KRÜGER, R. H.; QUIRINO, B. F. Xanthomonas campestris pv. campestris secretome toward susceptible and resistant Arabidopsis thaliana ecotypes. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 28., 2008; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 12., 2008; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 10., 2008; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 7., 2008, Londrina. FertBio 2008: desafios para o uso do solo com eficiência e qualidade ambiental: anais. Londrina: Embrapa Soja: SBCS: IAPAR: UEL, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 55. | | ARAÚJO, J. F.; CASTRO, A. R. de; COSTA, M. M. do C.; TOGAWA, R. C.; PAPPAS JUNIOR, G. J.; QUIRINO, B. F.; BUSTAMANTE, M. M. C.; WILLIAMSON, L.; HANDELSMAN, J.; KRUGER, R. H. Characterization of soil bacterial assemblies in Brazilian Savanna-Like vegetation reveals acidobacteria dominance. Microbial Ecology, v. 64, p. 760-770, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia. |
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| 56. | | CASTRO, A. P. de; ARAÚJO JÚNIOR, S. D.; REIS, A. M. M.; MOURA, R. L.; FRANCINI FILHO, R. B.; PAPPAS JUNIOR, G. J.; RODRIGUES, T. B.; THOMPSON, F. L.; KRÜGER, R. H. Bacterial community associated with healthy and diseased reef coral Mussismilia hispida from Eastern Brazil. Microbial Ecology, v. 59, p. 658-667, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 57. | | MENDES, I. V.; GARCIA, M. B.; BITENCOURT, A. C. A.; SANTANA, R. H.; LINS, P. de C.; SILVEIRA, R.; SIMMONS, B. A.; GLADDEN, J. M.; KRUGER, R. H.; QUIRINO, B. F. Bacterial diversity dynamics in microbial consortia selected for lignin utilization. PLoS ONE, v. 16, n. 9, e0255083, 2021. PDF: il. color.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 58. | | PALUAN, S. de F.; BITENCOURT, A. C. A.; PORTO, W. F.; SANTOS, D. F. K. dos; SOUTO, B. de M.; KRUGER, R. H.; FRANCO, O. L.; QUIRINO, B. F. Two glycosyl hydrolase clones isolated from a small-insert metagenomic library from Amazon soil environmental DNA. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 26., 2011, Foz do Iguaçu, PR. Anais... São Paulo: Sociedade Brasileira de Microbiologia, 2011. Não paginado.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 59. | | PALUAN, S. P.; SILVA, R. B.; CARVALHO, L. S.; SANTOS, D. F. K.; RAMOS, N. S. P. L.; BERGMAN, J. C.; NORONHA, E. F.; KRUGER, R. H.; QUIRINO, B. F. Enzymes of biotechnological interest prospected from a small insert metagenomic library constructed with Amazon soil microbial DNA. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR – SBBq, 39.; LATIN AMERICAN SYMPOSIUM ON THE MOLECULAR MECHANISMS OF SKELETAL MINERALIZATION, 1., 2010, Foz do Iguaçu, PR. Resumos... São Paulo: SBBq, 2010. Não paginado. Disponível em: < http://www.sbbq.org.br/2010/cdrom/busca.htm?query=quirino%2C+b.+f. >. Acesso em: 16 ago. 2010.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 60. | | QUIRINO, B. F.; BERGMANN, J. C.; SOUTO, B. de M.; TUPINAMBA, D.; RAMOS, T. G.; SCHROEDER, L. F.; PERIM, L.; NORONHA, E. F.; BARRETO, C. C.; KRUGER, R. H. Metagenomic glycosyl hydrolases from the Amazon soil and goat rumen. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 36., 2014, Flórida. Abstracts... Fairfax: Society for Industrial Microbiology and Biotechnology, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 64 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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