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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
19/12/2019 |
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Data da última atualização: |
28/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
JOSHI, L. R.; MOHR, K. A.; GAVA, D.; KUTISH, G.; BUYSSE, A. S.; VANNUCCI, F. A.; PIÑEYRO, P. E.; CROSSLEY, B. M.; SCHILTZ, J. J.; JENKINS-MOORE, M.; KOSTER, L.; TELL, R.; SCHAEFER, R.; MARTHALER, D.; DIEL, D. G. |
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Afiliação: |
LOK R. JOSHI, South Dakota State University; KRISTIN A. MOHR, South Dakota State University; DANIELLE GAVA, CNPSA; GERALD KUTISH, University of Connecticut; ALAIRE S. BUYSSE, South Dakota State University; FABIO A. VANNUCCI, University of Minnesota; PABLO E. PIÑEYRO, Iowa State University; BEATE M. CROSSLEY, University of California; JOHN J. SCHILTZ, USDA; MELINDA JENKINS-MOORE, USDA; LEO KOSTER, USDA; RACHEL TELL, USDA; REJANE SCHAEFER, CNPSA; DOUGLAS MARTHALER, Kansas State University; DIEGO G. DIEL, South Dakota State University. |
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Título: |
Genetic diversity and evolution of the emerging picornavirus Senecavirus A. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of General Virology, 20 dec. 2019. |
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DOI: |
10.1099/jgv.0.001360 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Senecavirus A (SVA) is an emerging picornavirus that causes vesicular disease (VD) in swine. The virus has been circulating in swine in the United Stated (USA) since at least 1988, however, since 2014 a marked increase in the number of SVA outbreaks has been observed in swine worldwide. The factors that led to the emergence of SVA remain unknown. Evolutionary changes that accumulated in the SVA genome over the years may have contributed to the recent increase in disease incidence. Here we compared full-genome sequences of historical SVA strains (identified before 2010) from the USA and global contemporary SVA strains (identified after 2011). The results from the genetic analysis revealed 6.32 % genetic divergence between historical and contemporary SVA isolates. Selection pressure analysis revealed that the SVA polyprotein is undergoing selection, with four amino acid (aa) residues located in the VP1 (aa 735), 2A (aa 941), 3C (aa 1547) and 3D (aa 1850) coding regions being under positive/diversifying selection. Several aa substitutions were observed in the structural proteins (VP1, VP2 and VP3) of contemporary SVA isolates when compared to historical SVA strains. Some of these aa substitutions led to changes in the surface electrostatic potential of the structural proteins. This work provides important insights into the molecular evolution and epidemiology of SVA. |
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Palavras-Chave: |
Genetic diversity. |
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Thesagro: |
Genética; Virologia; Vírus. |
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Thesaurus Nal: |
Seneca Valley virus; Senecavirus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02384naa a2200373 a 4500
001 2117248
005 2020-01-28
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1099/jgv.0.001360$2DOI
100 1 $aJOSHI, L. R.
245 $aGenetic diversity and evolution of the emerging picornavirus Senecavirus A.$h[electronic resource]
260 $c2019
520 $aAbstract: Senecavirus A (SVA) is an emerging picornavirus that causes vesicular disease (VD) in swine. The virus has been circulating in swine in the United Stated (USA) since at least 1988, however, since 2014 a marked increase in the number of SVA outbreaks has been observed in swine worldwide. The factors that led to the emergence of SVA remain unknown. Evolutionary changes that accumulated in the SVA genome over the years may have contributed to the recent increase in disease incidence. Here we compared full-genome sequences of historical SVA strains (identified before 2010) from the USA and global contemporary SVA strains (identified after 2011). The results from the genetic analysis revealed 6.32 % genetic divergence between historical and contemporary SVA isolates. Selection pressure analysis revealed that the SVA polyprotein is undergoing selection, with four amino acid (aa) residues located in the VP1 (aa 735), 2A (aa 941), 3C (aa 1547) and 3D (aa 1850) coding regions being under positive/diversifying selection. Several aa substitutions were observed in the structural proteins (VP1, VP2 and VP3) of contemporary SVA isolates when compared to historical SVA strains. Some of these aa substitutions led to changes in the surface electrostatic potential of the structural proteins. This work provides important insights into the molecular evolution and epidemiology of SVA.
650 $aSeneca Valley virus
650 $aSenecavirus
650 $aGenética
650 $aVirologia
650 $aVírus
653 $aGenetic diversity
700 1 $aMOHR, K. A.
700 1 $aGAVA, D.
700 1 $aKUTISH, G.
700 1 $aBUYSSE, A. S.
700 1 $aVANNUCCI, F. A.
700 1 $aPIÑEYRO, P. E.
700 1 $aCROSSLEY, B. M.
700 1 $aSCHILTZ, J. J.
700 1 $aJENKINS-MOORE, M.
700 1 $aKOSTER, L.
700 1 $aTELL, R.
700 1 $aSCHAEFER, R.
700 1 $aMARTHALER, D.
700 1 $aDIEL, D. G.
773 $tJournal of General Virology, 20 dec. 2019.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
20/04/2010 |
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Data da última atualização: |
01/11/2021 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
NOVAES, H. B.; VAITSMAN, D. S.; DUTRA, P. B.; PEREZ, D. V. |
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Afiliação: |
HUMBERTO BREVILATO NOVAES, UFRJ; DELMO SANTIAGO VAITSMAN, UFRJ; PAULO BECHARA DUTRA, UFRJ; DANIEL VIDAL PEREZ, CNPS. |
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Título: |
Determination of nitrate in lettuce by ion chromatography after microwave water extraction. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Química Nova, São Paulo, v. 32, n. 6, p. 1647-1650, 2009. |
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DOI: |
https://doi.org/10.1590/S0100-40422009000600049 |
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Idioma: |
Inglês |
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Conteúdo: |
Lettuce is worldwide known as the most important vegetable. In this context, most farmers are searching new techniques for best quality products including hydropony. However, nitrate is of great concern, since it has a negative impact on human metabolism. The main objective of the present work was to evaluate the nitrate content of lettuce produced by conventional and hydroponic systems. The determination was conducted by ion chromatography and a new method of extraction was tested using microwave oven digestion. The results indicated that nitrate level produced in the conventional system was lower than in the hydroponic system. |
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Palavras-Chave: |
Chromatrography. |
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Thesagro: |
Cromatografia. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/184915/1/Daniel-Revista-Quimica-Nova.pdf
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Marc: |
LEADER 01254naa a2200193 a 4500
001 1697117
005 2021-11-01
008 2009 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S0100-40422009000600049$2DOI
100 1 $aNOVAES, H. B.
245 $aDetermination of nitrate in lettuce by ion chromatography after microwave water extraction.$h[electronic resource]
260 $c2009
520 $aLettuce is worldwide known as the most important vegetable. In this context, most farmers are searching new techniques for best quality products including hydropony. However, nitrate is of great concern, since it has a negative impact on human metabolism. The main objective of the present work was to evaluate the nitrate content of lettuce produced by conventional and hydroponic systems. The determination was conducted by ion chromatography and a new method of extraction was tested using microwave oven digestion. The results indicated that nitrate level produced in the conventional system was lower than in the hydroponic system.
650 $aCromatografia
653 $aChromatrography
700 1 $aVAITSMAN, D. S.
700 1 $aDUTRA, P. B.
700 1 $aPEREZ, D. V.
773 $tQuímica Nova, São Paulo$gv. 32, n. 6, p. 1647-1650, 2009.
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