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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
18/08/1993 |
Data da última atualização: |
18/08/1993 |
Autoria: |
ABRAO, J. R.; KORNDORFER, G. H. |
Título: |
Efeito residual e acumulativo da adubacao potasica, na sucessao trigo-soja, em oxissolo do planalto riograndense. |
Ano de publicação: |
1980 |
Fonte/Imprenta: |
In: REUNIAO NACIONAL DE PESQUISA DE TRIGO, 11., 1980, Porto Alegre. Contribuicao de Centro de Experimentacao e Pesquisa a XI Reuniao Nacional de Pesquisa de Trigo. Cruz Alta: FECOTRIGO, 1980. |
Páginas: |
p.158-172. |
Idioma: |
Português |
Palavras-Chave: |
Brasil; Double-cropping; Fertilization; Residual effect; Rio Grande do Sul; Soybean; Sucessao. |
Thesagro: |
Adubação; Efeito Residual; Potássio; Soja; Trigo. |
Thesaurus Nal: |
Brazil; potassium; wheat. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00970naa a2200313 a 4500 001 1451150 005 1993-08-18 008 1980 bl uuuu u00u1 u #d 100 1 $aABRAO, J. R. 245 $aEfeito residual e acumulativo da adubacao potasica, na sucessao trigo-soja, em oxissolo do planalto riograndense. 260 $c1980 300 $ap.158-172. 650 $aBrazil 650 $apotassium 650 $awheat 650 $aAdubação 650 $aEfeito Residual 650 $aPotássio 650 $aSoja 650 $aTrigo 653 $aBrasil 653 $aDouble-cropping 653 $aFertilization 653 $aResidual effect 653 $aRio Grande do Sul 653 $aSoybean 653 $aSucessao 700 1 $aKORNDORFER, G. H. 773 $tIn: REUNIAO NACIONAL DE PESQUISA DE TRIGO, 11., 1980, Porto Alegre. Contribuicao de Centro de Experimentacao e Pesquisa a XI Reuniao Nacional de Pesquisa de Trigo. Cruz Alta: FECOTRIGO, 1980.
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Embrapa Soja (CNPSO) |
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Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
14/05/2004 |
Data da última atualização: |
14/05/2004 |
Autoria: |
DE SOUZA, F. A.; KOWALCHUCK, G. A.; LEEFLANG, P.; VAN VEEN, J. A.; SMIT, E. |
Título: |
PCR-denaturing gradient gel electrophoresis profiling of inter- and intraspecies 18S rRNA gene sequence heterogeneity is an accurate and sensitive method to assess species diversity of arbuscular mycorrhizal fungi of the genus Gigaspora. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
Applied and Environmental microbiology, Washington, v. 70, n. 3, p. 1413-1424, mar. 2004 |
Idioma: |
Inglês |
Conteúdo: |
Despite the importance of arbuscular mycorrhizal fungi in the majority of terrestrial ecosystems, their ecology, genetics, and evolution are poorly understood, partly due to difficulties associated with detecting and identifying species. We explored the inter and intraspecies variations of the variations of the 18S r DNA genes of the genus Gigaspora to assess the use of this marker for the discrimination of Gigaspora isolates and of Gigasporaceae populations from environmental samples. Screening of 48 Gigaspora isolates by PCR-denaturing gradient gel electrophoresis (DGGE) revealed that the V3-V4 region of the 18S rDNA gene contained insufficient variation to discriminate between different Gigaspora species. In contrast, the patterns of 18S ribosomal DNA (rDNA) heterogeneity within the V9 region of this marker could be used for reliable identification of all recognized species within the genus. PCR-DGGE patterns provided insight into some putative misidentifications and could be used to differentiate geographic isolates of G. albida, G. gigantea, and G margarita but not G. rosea. Two major clusters were apparent based upon PCR-DGGE ribotype patterns, one containing G. albida, G. candida, G. ramisporophora, and G. rosea and the other containing G. decipiens and G. margarita. Dissection of the DGGE patterns by cloning. DGGE screening, and sequencing confirmed these groupings and revealed that some ribotypes were shared across species boundaries. Of the 48 isolates examined, only two displayed any spore-to-spore variation, and these exceptions may be indicative of coisolation of more than one species or subspecies within these cultures. Two Brazilian agricultural soils were also analyzed with a Gigasporaceae-specific nested PCR approach, revealing a dominance of G. margarita within this family. MenosDespite the importance of arbuscular mycorrhizal fungi in the majority of terrestrial ecosystems, their ecology, genetics, and evolution are poorly understood, partly due to difficulties associated with detecting and identifying species. We explored the inter and intraspecies variations of the variations of the 18S r DNA genes of the genus Gigaspora to assess the use of this marker for the discrimination of Gigaspora isolates and of Gigasporaceae populations from environmental samples. Screening of 48 Gigaspora isolates by PCR-denaturing gradient gel electrophoresis (DGGE) revealed that the V3-V4 region of the 18S rDNA gene contained insufficient variation to discriminate between different Gigaspora species. In contrast, the patterns of 18S ribosomal DNA (rDNA) heterogeneity within the V9 region of this marker could be used for reliable identification of all recognized species within the genus. PCR-DGGE patterns provided insight into some putative misidentifications and could be used to differentiate geographic isolates of G. albida, G. gigantea, and G margarita but not G. rosea. Two major clusters were apparent based upon PCR-DGGE ribotype patterns, one containing G. albida, G. candida, G. ramisporophora, and G. rosea and the other containing G. decipiens and G. margarita. Dissection of the DGGE patterns by cloning. DGGE screening, and sequencing confirmed these groupings and revealed that some ribotypes were shared across species boundaries. Of the 48 isolates examined, on... Mostrar Tudo |
Palavras-Chave: |
Vesicular arbuscular mycorrhizal. |
Thesagro: |
Micorriza Vesicular Arbuscular. |
Thesaurus NAL: |
Gigaspora. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02615naa a2200205 a 4500 001 1625782 005 2004-05-14 008 2004 bl --- 0-- u #d 100 1 $aDE SOUZA, F. A. 245 $aPCR-denaturing gradient gel electrophoresis profiling of inter- and intraspecies 18S rRNA gene sequence heterogeneity is an accurate and sensitive method to assess species diversity of arbuscular mycorrhizal fungi of the genus Gigaspora. 260 $c2004 520 $aDespite the importance of arbuscular mycorrhizal fungi in the majority of terrestrial ecosystems, their ecology, genetics, and evolution are poorly understood, partly due to difficulties associated with detecting and identifying species. We explored the inter and intraspecies variations of the variations of the 18S r DNA genes of the genus Gigaspora to assess the use of this marker for the discrimination of Gigaspora isolates and of Gigasporaceae populations from environmental samples. Screening of 48 Gigaspora isolates by PCR-denaturing gradient gel electrophoresis (DGGE) revealed that the V3-V4 region of the 18S rDNA gene contained insufficient variation to discriminate between different Gigaspora species. In contrast, the patterns of 18S ribosomal DNA (rDNA) heterogeneity within the V9 region of this marker could be used for reliable identification of all recognized species within the genus. PCR-DGGE patterns provided insight into some putative misidentifications and could be used to differentiate geographic isolates of G. albida, G. gigantea, and G margarita but not G. rosea. Two major clusters were apparent based upon PCR-DGGE ribotype patterns, one containing G. albida, G. candida, G. ramisporophora, and G. rosea and the other containing G. decipiens and G. margarita. Dissection of the DGGE patterns by cloning. DGGE screening, and sequencing confirmed these groupings and revealed that some ribotypes were shared across species boundaries. Of the 48 isolates examined, only two displayed any spore-to-spore variation, and these exceptions may be indicative of coisolation of more than one species or subspecies within these cultures. Two Brazilian agricultural soils were also analyzed with a Gigasporaceae-specific nested PCR approach, revealing a dominance of G. margarita within this family. 650 $aGigaspora 650 $aMicorriza Vesicular Arbuscular 653 $aVesicular arbuscular mycorrhizal 700 1 $aKOWALCHUCK, G. A. 700 1 $aLEEFLANG, P. 700 1 $aVAN VEEN, J. A. 700 1 $aSMIT, E. 773 $tApplied and Environmental microbiology, Washington$gv. 70, n. 3, p. 1413-1424, mar. 2004
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