| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
15/03/2017 |
|
Data da última atualização: |
15/03/2017 |
|
Autoria: |
KAWANO, A.; TATEYAMA, S.; YAMAGUCHI, R.; NOSAKA, D.; KONDO, F. |
|
Título: |
Morphology of goat milk-derived mammary epithelial cells cultured in collagen gel. |
|
Ano de publicação: |
1988 |
|
Fonte/Imprenta: |
Japanese Journal of Veterinary Science v. 50, n. 6, p. 1252-1258, Dec. 1988. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: Mammary epithelial cells derived from goat milk were cultured without contamination of fibroblasts using the collagen gel. Cells grown on the fixed collagen gel showed similar morphology to those grown in plate cultures, with elongation and pleomorphism and producing a monolayer. After detaching the gels from the dishes cells became rounded. No cellular growth was seen when embedded in the collagen gel. Cells grown on both fixed and floating gel cultures were positive for antikeratin and antiactin immune serum. By electron microscopy bells grown on the fixed gel culture had poor organelles, whereas those on floating gel cultures had moderately developed Golgi apparatuses and laminar structures. No secretory granules were seen in any type of cultures with collagen gel. The floating collagen gel culture might be useful for in vitro studies on differentiation of mammary epithelial cells. |
|
Palavras-Chave: |
Collagen gel; Mammary epithelium. |
|
Thesagro: |
Caprino; Colágeno; Glândula mamária; Leite de cabra. |
|
Thesaurus Nal: |
Gels; Goat milk; Mammary glands. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 01657naa a2200277 a 4500
001 2067042
005 2017-03-15
008 1988 bl uuuu u00u1 u #d
100 1 $aKAWANO, A.
245 $aMorphology of goat milk-derived mammary epithelial cells cultured in collagen gel.
260 $c1988
520 $aAbstract: Mammary epithelial cells derived from goat milk were cultured without contamination of fibroblasts using the collagen gel. Cells grown on the fixed collagen gel showed similar morphology to those grown in plate cultures, with elongation and pleomorphism and producing a monolayer. After detaching the gels from the dishes cells became rounded. No cellular growth was seen when embedded in the collagen gel. Cells grown on both fixed and floating gel cultures were positive for antikeratin and antiactin immune serum. By electron microscopy bells grown on the fixed gel culture had poor organelles, whereas those on floating gel cultures had moderately developed Golgi apparatuses and laminar structures. No secretory granules were seen in any type of cultures with collagen gel. The floating collagen gel culture might be useful for in vitro studies on differentiation of mammary epithelial cells.
650 $aGels
650 $aGoat milk
650 $aMammary glands
650 $aCaprino
650 $aColágeno
650 $aGlândula mamária
650 $aLeite de cabra
653 $aCollagen gel
653 $aMammary epithelium
700 1 $aTATEYAMA, S.
700 1 $aYAMAGUCHI, R.
700 1 $aNOSAKA, D.
700 1 $aKONDO, F.
773 $tJapanese Journal of Veterinary Science$gv. 50, n. 6, p. 1252-1258, Dec. 1988.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 1 | |
| 1. |  | KOMIYA, S.; ARAUJO, A. C. de; LAVRIC, J. V.; NELSON, B.; SÖRGEL, M.; WEBER, B.; BOTIA, S.; GOMES-ALVES, E.; WALTER, D.; SÁ, M. de O.; WOLFF, S.; PINHO, D. M.; KONDO, F.; TRUMBORE, S. Seasonal and interannual variations of carbon fluxes at the Amazon Tall Tower Observatory site in 2014-2019. In: EGU GENERAL ASSEMBLY, 2022, Viena. Programme. [S.l.]: EGU, 2022.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
|    |
| Registros recuperados : 1 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
