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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/11/2012 |
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Data da última atualização: |
18/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LIU, J.; LUO, X.; SHAFF, J.; LIANG, C.; JIA, X.; LI, Z.; MAGALHAES, J.; KOCHIAN, L. V. |
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Afiliação: |
Jiping Liu, Cornell University, Ithaca, NY.; Xiaoying Luo, Southwest University, CHINA.; Jon Shaff, Cornell University, USA; Cuiyue Liang, South China Agriculture University, China.; Xiaomin Jia, Cornell University, USA.; Ziyan Li, Northwest Sci-Tech University of Agriculture and Forestry, China; JURANDIR VIEIRA DE MAGALHAES, CNPMS; Leon V. Kochian, Cornell University, USA. |
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Título: |
A promoter-swap strategy between the AtALMT and AtMATE genes increased Arabidopsis aluminum resistance and improved carbon-use efficiency for aluminum resistance. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
The Plant Journal, Oxford, v. 71, p. 327-337, 2012. |
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DOI: |
10.1111/j.1365-313X.2012.04994.x |
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Idioma: |
Inglês |
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Conteúdo: |
The primary mechanism of Arabidopsis aluminum (Al) resistance is based on root Al exclusion, resulting from Al-activated root exudation of the Al3+-chelating organic acids, malate and citrate. Root malate exudation is the major contributor to Arabidopsis Al resistance, and is conferred by expression of AtALMT1, which encodes the root malate transporter. Root citrate exudation plays a smaller but still significant role in Arabidopsis Al resistance, and is conferred by expression of AtMATE, which encodes the root citrate transporter. In this study, we demonstrate that levels of Al-activated root organic acid exudation are closely correlated with expression of the organic acid transporter genes AtALMT1 and AtMATE. We also found that the AtALMT1 promoter confers a significantly higher level of gene expression than the AtMATE promoter. Analysis of AtALMT1 and AtMATE tissue- and cell-specific expression based on stable expression of promoter–reporter gene constructs showed that the two genes are expressed incomplementary root regions: AtALMT1 is expressed in the root apices, while AtMATE is expressed in the mature portions of the roots. As citrate is a much more effective chelator of Al3+ than malate, we used a promoter-swap strategy to test whether root tip-localized expression of the AtMATE coding region driven by the stronger AtALMT1 promoter (AtALMT1P::AtMATE) resulted in increased Arabidopsis Al resistance. Our results indicate that expression of AtALMT1P::AtMATE not only significantly increased Al resistance of the transgenic plants, but also enhanced carbon-use efficiency for Al resistance. MenosThe primary mechanism of Arabidopsis aluminum (Al) resistance is based on root Al exclusion, resulting from Al-activated root exudation of the Al3+-chelating organic acids, malate and citrate. Root malate exudation is the major contributor to Arabidopsis Al resistance, and is conferred by expression of AtALMT1, which encodes the root malate transporter. Root citrate exudation plays a smaller but still significant role in Arabidopsis Al resistance, and is conferred by expression of AtMATE, which encodes the root citrate transporter. In this study, we demonstrate that levels of Al-activated root organic acid exudation are closely correlated with expression of the organic acid transporter genes AtALMT1 and AtMATE. We also found that the AtALMT1 promoter confers a significantly higher level of gene expression than the AtMATE promoter. Analysis of AtALMT1 and AtMATE tissue- and cell-specific expression based on stable expression of promoter–reporter gene constructs showed that the two genes are expressed incomplementary root regions: AtALMT1 is expressed in the root apices, while AtMATE is expressed in the mature portions of the roots. As citrate is a much more effective chelator of Al3+ than malate, we used a promoter-swap strategy to test whether root tip-localized expression of the AtMATE coding region driven by the stronger AtALMT1 promoter (AtALMT1P::AtMATE) resulted in increased Arabidopsis Al resistance. Our results indicate that expression of AtALMT1P::AtMATE not only sig... Mostrar Tudo |
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Palavras-Chave: |
Aluminum resistance; Carbon-use efficiency; Organic acid exudation; Promoter swap; Transporter protein. |
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Thesaurus Nal: |
Arabidopsis thaliana. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02528naa a2200289 a 4500
001 1940816
005 2017-05-18
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1111/j.1365-313X.2012.04994.x$2DOI
100 1 $aLIU, J.
245 $aA promoter-swap strategy between the AtALMT and AtMATE genes increased Arabidopsis aluminum resistance and improved carbon-use efficiency for aluminum resistance.$h[electronic resource]
260 $c2012
520 $aThe primary mechanism of Arabidopsis aluminum (Al) resistance is based on root Al exclusion, resulting from Al-activated root exudation of the Al3+-chelating organic acids, malate and citrate. Root malate exudation is the major contributor to Arabidopsis Al resistance, and is conferred by expression of AtALMT1, which encodes the root malate transporter. Root citrate exudation plays a smaller but still significant role in Arabidopsis Al resistance, and is conferred by expression of AtMATE, which encodes the root citrate transporter. In this study, we demonstrate that levels of Al-activated root organic acid exudation are closely correlated with expression of the organic acid transporter genes AtALMT1 and AtMATE. We also found that the AtALMT1 promoter confers a significantly higher level of gene expression than the AtMATE promoter. Analysis of AtALMT1 and AtMATE tissue- and cell-specific expression based on stable expression of promoter–reporter gene constructs showed that the two genes are expressed incomplementary root regions: AtALMT1 is expressed in the root apices, while AtMATE is expressed in the mature portions of the roots. As citrate is a much more effective chelator of Al3+ than malate, we used a promoter-swap strategy to test whether root tip-localized expression of the AtMATE coding region driven by the stronger AtALMT1 promoter (AtALMT1P::AtMATE) resulted in increased Arabidopsis Al resistance. Our results indicate that expression of AtALMT1P::AtMATE not only significantly increased Al resistance of the transgenic plants, but also enhanced carbon-use efficiency for Al resistance.
650 $aArabidopsis thaliana
653 $aAluminum resistance
653 $aCarbon-use efficiency
653 $aOrganic acid exudation
653 $aPromoter swap
653 $aTransporter protein
700 1 $aLUO, X.
700 1 $aSHAFF, J.
700 1 $aLIANG, C.
700 1 $aJIA, X.
700 1 $aLI, Z.
700 1 $aMAGALHAES, J.
700 1 $aKOCHIAN, L. V.
773 $tThe Plant Journal, Oxford$gv. 71, p. 327-337, 2012.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 53 | |
| 1. |  | CANIATO, F. F.; GUIMARAES, C. T.; SCHAFFERT, R. E.; KOCHIAN, L. V.; MAGALHAES, J. V. D. Association mapping for aluminum tolerance in candidate regions of the Altsb locus in sorghum. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 150.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 3. | | PINEROS, M. A.; SHAFF, J. E.; MANSLANK, H. S.; ALVES, V. M. C.; KOCHIAN, L. V. Aluminum resistance in maize cannot be solely explained by root organic acid exudation. A comparative physiological study Plant Physiology, Bethesda, v. 137, n. 1, p. 231-241, 2005.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
|  |
| 6. | | MAGALHAES, J. V.; SOUSA, S. M. de; GUIMARAES, C. T.; KOCHIAN, L. V. The role of root morphology and architecture in phosphorus acquisiton: physiological, genetic, and molecular basis. In: HOSSAIN, M. A.; KAMIYA, T.; BURRITT, D. J.; PHAN TRAN, L.-S.; FUJIWARA, T. (Ed.). Plant macronutrient use efficiency: molecular and genomic perspectives in crop plants. London: Academic Press, 2017. cap. 7, p. 123-147.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 10. | | CANIATO, F. F.; HAMBLIN, M. T.; GUIMARAES, C. T.; ZHANG, Z.; SCHAFFERT, R. E.; KOCHIAN, L. V.; MAGALHAES, J. V. Association mapping provides insights into the origin and the fine structure of the sorghum aluminum tolerance locus, AltSB. Plos One, San Francisco, v. 9, n. 1, p. 1-12, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 11. | | ALVES, V. M. C.; MAGALHAES, J. V. de; KOCHIAN, L. V.; PARENTONI, S. N.; PAIVA, E.; GARVIN, D. F. Acumulação de alumínio em ápices de raízes de milho. In: REUNIAO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRICAO DE PLANTAS, 24.; REUNIAO BRASILEIRA SOBRE MICORRIZAS, 8.; SIMPOSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 6.; REUNIAO BRASILEIRA DE BIOLOGIA DO SOLO, 3., 2000, Santa Maria, RS. Biodinâmica do solo: guia do congressista. [Viçosa, MG]: Sociedade Brasileira de Ciência do Solo; [São Paulo]: Sociedade Brasileira de Microbiologia; Santa Maria, RS: Universidade Federal de Santa Maria, Departamento de Solos, 2000. Fertbio 2000. p. 151.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 12. | | HUFNAGEL, B.; GUIMARÃES, C. T.; CRAFT, E. J.; SHAFF, J. E.; SCHAFFERT, R. E.; KOCHIAN, L. V.; MAGALHAES, J. V. Exploiting sorghum genetic diversity for enhanced aluminum tolerance: allele mining based on the AltSB locus. Scientific Reports, v. 8, p. 1-13, July 2018. Article number: 10094.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 13. | | ALVES, V. M. C.; MAGALHAES, J. V. de; SHAFF, J.; SCHAFFERT, R. E.; GUIMARAES, C. T.; KOCHIAN, L. V. Exsudação de citrato e tolerânica ao alumínio em sorgo. In: CONGRESSO NACIONAL DE MILHO E SORGO, 26.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 2.; SIMPÓSIO SOBRE COLLETOTRICHUM GRAMINICOLA, 1., 2006, Belo Horizonte. Inovação para sistemas integrados de produção: trabalhos apresentados. [Sete Lagoas]: ABMS, 2006. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 14. | | PAPERNIK, L. A.; BETHEA, A. S.; SINGLETON, T. E.; MAGALHAES, J. V.; GARVIN, D. F.; KOCHIAN, L. V. Physiological basis of reduced Al tolerance in ditelosomic lines of Chinese spring wheat. Planta, New York, v. 212, n. 5/6, p. 829-834, 2001.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 15. | | LIU, J.; LUO, X.; SHAFF, J.; LIANG, C.; JIA, X.; LI, Z.; MAGALHAES, J.; KOCHIAN, L. V. A promoter-swap strategy between the AtALMT and AtMATE genes increased Arabidopsis aluminum resistance and improved carbon-use efficiency for aluminum resistance. The Plant Journal, Oxford, v. 71, p. 327-337, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 16. | | MAGALHAES, J. V.; GARVIN, D. F.; WANG, Y. H.; SORRELLS, M. E.; KLEIN, P. E.; SCHAFFERT, R. E.; LI, L.; KOCHIAN, L. V. Comparative mapping of a major aluminum tolerance gene in sorghum and other species in the poaceae. Genetics, Maryland, v. 167, n. 4, p. 1905-1914, 2004.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 17. | | LIU, Z.; QIN, T.; ATIENZA, M.; ZHAO, Y.; NGUYEN, H.; SHENG, H.; OLUKAYODE, O.; SONG, H.; PANJVANI, K.; MAGALHAES, J. V. de; LUCAS, W. J.; KOCHIAN, L. V. Constitutive basis of root system architecture: uncovering a promising trait for breeding nutrient-and drought-resilient crops. aBIOTECH, v. 4, p. 315–331, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 3 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 18. | | CANÇADO, G. M. A.; PINEROS, M. A.; MARON, L. G.; SHAFF, J.; CAMARGO, S. R.; MENOSSI, M.; ALVES, V. M. C.; KOCHIAN, L. V. Cloning and characterization of an ALMT1 homologue gene in Maize. In: INTERNATIONAL PLANT & ANIMAL GENOMES CONFERENCE, 15., 2007, San Diego, CA. [Proceedings...]. [S. l.: s.n.], 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 19. | | LANA, U. G. de P.; GUIMARAES, C. T.; ALVES, V. M. C.; SCHAFFERT, R. E.; KOCHIAN, L. V.; MAGALHAES, J. V. de. Caracterização molecular do gene SbMATE, principal responsável pela tolerância ao alumínio em sorgo. In: CONGRESSO NACIONAL DE MILHO E SORGO, 27.; SIMPOSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 3.; WORKSHOP SOBRE MANEJO E ETIOLOGIA DA MANCHA BRANCA DO MILHO, 2008, Londrina. Agroenergia, produção de alimentos e mudanças climáticas: desafios para milho e sorgo: trabalhos e palestras. [Londrina]: IAPAR; [Sete Lagoas]: Embrapa Milho e Sorgo, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 20. | | LANA, U. G. D. P.; GUIMARAES, C. T.; ALVES, V. M. C.; SCHAFFERT, R. E.; KOCHIAN, L. V.; MAGALHAES, J. V. D. Caracterização molecular de um membro da família Multidrug and Toxic Compound Extrusion (MATE) que confere tolerância ao alumínio em sorgo. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 53.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 53 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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