Data/hora: 15/06/2024 04:39:23

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 22/11/1999
Data da última atualização: | 22/11/1999
Autoria: | KOBAYASHI, S.; UCHIMIYA, H.
Afiliação: | Akitsu Branch, Fruit Tree Research Station, Akitsu, Hiroshima 729-24; Institute of Biological Sciences, University of Tsukuba, Tsukuba City, Ibaraki 305.
Título: | Expression and integration of a foreign gene in orange (Citrus sinensis Osb.) protoplasts by direct DNA transfer
Ano de publicação: | 1989
Fonte/Imprenta: | Jpn. J. Genet, v.64, p.91-97, 1989
Idioma: | Inglês
Conteúdo: | Protoplasts isolated from suspension cultured cells of orange (Citrus sinensis Osb.) were treated with a bacterial plasmid DNA carrying a chimeric gene consisting of the nopaline synthase promoter, the aminoglycoside phosphotrans-ferase II [APH(3) II] structural gene from the bacterial transposon Tn 5 and a terminator region from cauliflower mosaic virus DNA. Colonies capable of proliferating in a medium containing kanamycin (25 ug/ml) possessed APH (3) II activity and an intact foreign gene. The transformation frequency was recorded to be in the order of 10-6.
Palavras-Chave: | Citrus sinensis Osb; Cultur de tecidos; Hibridos somaticos; Orange; Protoplastos; Somatic hydrid; Tissus culture.
Thesagro: | Laranja.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (17428-1) - ADD - SP - -- - S11630 -  - S11630 - DIS