Data/hora: 15/06/2024 04:24:32

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 04/09/1995
Data da última atualização: | 04/09/1995
Autoria: | KOBAYASHI, S.; UCHIMIYA, H.; IKEDA, I.
Afiliação: | Institute of Biological Sciences, University of Tsukuba, Sakura-mura, Ibaraki 305, Japan.
Título: | Plant regeneration from `Trovita' orange protoplasts.
Ano de publicação: | 1983
Fonte/Imprenta: | Japan. J. Breed., v.33, n.2, p.119-122, 1983.
Idioma: | Inglês
Conteúdo: | Protoplasts isolated from nucellar callus of `Trovita' orange (Citrus sinensis Osbeck) were cultured in a Murashige and Tucker's medium containing 0.15 M sucrose, 0.45 M glucose, and 0.6% agar, but leaking phytohormones. Colonies with 26.3% plating efficiency were observed after 6 weeks culture. When the protoplasts were cultured in the presence of exogenous phytohormones, reduction in colony formation was observed. Some colonies developed to green embryoids. which eventually formed plants.
Palavras-Chave: | Citrus sinensis Osbeck; Nucellar callus; Plant regeneration; Preservation; Protoplast.
Thesaurus Nal: | embryogenesis.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (15988-1) - ADC - SP - -- - S10310 -  - S10310 - DIS