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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
27/05/2016 |
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Data da última atualização: |
29/01/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
TULLIO, L. D.; PAULITSCH, F.; REICHERT, P. R. S.; KLEPA, M. S.; KLEPA, M. S.; PILEGGI, M.; GOMES, D. F.; HUNGRIA, M.; BATISTA, J. S. S. |
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Afiliação: |
UEPG; UEPG; UEPG; UEPG; UEPG; MARIANGELA HUNGRIA DA CUNHA, CNPSO; UEPG. |
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Título: |
Proteomic analysis of Rhizobium freirei PRF 81 responses to low pH. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28.; SIMPÓSIO DE FERMENTAÇÃO ALCOÓLICA, 3.; SIMPÓSIO DE MICRORGANISMOS FOTOSSINTETIZANTES, 3.; SIMPÓSIO DE ESCHERICHIA COLI LUIZ RACHID TRABULSI, 4., 2015, Florianópolis. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia, 2015. |
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Idioma: |
Português |
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Notas: |
Res. 1407-2. |
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Conteúdo: |
Rhizobium freirei PRF 81 is employed in common bean commercial inoculants in Brazil, due to its outstanding efficiency in fixing nitrogen, competitiveness and tolerance to abiotic stresses. Among the environmental conditions faced by rhizobia in soils, acidity is perhaps the encountered most, especially in Brazil. So, we used proteomics based approaches to study the responses of PRF 81 to a low pH condition. R. freirei PRF 81 was grown in TY medium until exponential phase in two treatments: pH 6,8 and pH 4,8. Whole-cell proteins were extracted and separated by two-dimensional gel electrophoresis, using IPG-strips with pH range 4-7 and 12% polyacrilamide gels. The experiment was performed in triplicate. Protein spots were detected in the high-resolution digitized gel images and analyzed by Image Master 2D Platinum v 5.0 software. Relative volumes (%vol) of compared between the two conditions tested and were statistically evaluated (p ≤ 0.05). Even knowing that R. freirei PRF 81 can still grow in more acid conditions, pH 4.8 was chosen because didn´t affect significantly the bacterial growth kinetics, a factor that could compromise the analysis. Using a narrow pH range, the gel profiles displayed a better resolution and reprodutibility than using broader pH range. Spots were mostly concentrated between pH 5-7 and molecular masses between 17-95 kDa. From the six hundred well-defined spots analyzed, one hundred and sixty-three spots presented a significant change in % vol, indicating that the pH led to expressive changes in the proteome of R. freirei PRF 81. Of these, sixty-one were up-regulated and one hundred two was downregulated in pH 4.8 condition. Also, fourteen spots were only identified in the acid condition, while seven spots was exclusively detected in pH 6.8. Ninety-five differentially expressed spots and two exclusively detected in pH 4,8 were selected for Maldi-Tof identification. Together with the genome sequencing and the proteome analysis of heat stress, we will search for molecular determinants of PRF 81 related to capacity to adapt to stressful tropical conditions. MenosRhizobium freirei PRF 81 is employed in common bean commercial inoculants in Brazil, due to its outstanding efficiency in fixing nitrogen, competitiveness and tolerance to abiotic stresses. Among the environmental conditions faced by rhizobia in soils, acidity is perhaps the encountered most, especially in Brazil. So, we used proteomics based approaches to study the responses of PRF 81 to a low pH condition. R. freirei PRF 81 was grown in TY medium until exponential phase in two treatments: pH 6,8 and pH 4,8. Whole-cell proteins were extracted and separated by two-dimensional gel electrophoresis, using IPG-strips with pH range 4-7 and 12% polyacrilamide gels. The experiment was performed in triplicate. Protein spots were detected in the high-resolution digitized gel images and analyzed by Image Master 2D Platinum v 5.0 software. Relative volumes (%vol) of compared between the two conditions tested and were statistically evaluated (p ≤ 0.05). Even knowing that R. freirei PRF 81 can still grow in more acid conditions, pH 4.8 was chosen because didn´t affect significantly the bacterial growth kinetics, a factor that could compromise the analysis. Using a narrow pH range, the gel profiles displayed a better resolution and reprodutibility than using broader pH range. Spots were mostly concentrated between pH 5-7 and molecular masses between 17-95 kDa. From the six hundred well-defined spots analyzed, one hundred and sixty-three spots presented a significant chan... Mostrar Tudo |
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Thesagro: |
Fixação de nitrogênio. |
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Thesaurus Nal: |
Nitrogen fixation. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/143444/1/R1407-2.PDF
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Marc: |
LEADER 03060nam a2200241 a 4500
001 2045758
005 2018-01-29
008 2015 bl uuuu u00u1 u #d
100 1 $aTULLIO, L. D.
245 $aProteomic analysis of Rhizobium freirei PRF 81 responses to low pH.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28.; SIMPÓSIO DE FERMENTAÇÃO ALCOÓLICA, 3.; SIMPÓSIO DE MICRORGANISMOS FOTOSSINTETIZANTES, 3.; SIMPÓSIO DE ESCHERICHIA COLI LUIZ RACHID TRABULSI, 4., 2015, Florianópolis. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia$c2015
500 $aRes. 1407-2.
520 $aRhizobium freirei PRF 81 is employed in common bean commercial inoculants in Brazil, due to its outstanding efficiency in fixing nitrogen, competitiveness and tolerance to abiotic stresses. Among the environmental conditions faced by rhizobia in soils, acidity is perhaps the encountered most, especially in Brazil. So, we used proteomics based approaches to study the responses of PRF 81 to a low pH condition. R. freirei PRF 81 was grown in TY medium until exponential phase in two treatments: pH 6,8 and pH 4,8. Whole-cell proteins were extracted and separated by two-dimensional gel electrophoresis, using IPG-strips with pH range 4-7 and 12% polyacrilamide gels. The experiment was performed in triplicate. Protein spots were detected in the high-resolution digitized gel images and analyzed by Image Master 2D Platinum v 5.0 software. Relative volumes (%vol) of compared between the two conditions tested and were statistically evaluated (p ≤ 0.05). Even knowing that R. freirei PRF 81 can still grow in more acid conditions, pH 4.8 was chosen because didn´t affect significantly the bacterial growth kinetics, a factor that could compromise the analysis. Using a narrow pH range, the gel profiles displayed a better resolution and reprodutibility than using broader pH range. Spots were mostly concentrated between pH 5-7 and molecular masses between 17-95 kDa. From the six hundred well-defined spots analyzed, one hundred and sixty-three spots presented a significant change in % vol, indicating that the pH led to expressive changes in the proteome of R. freirei PRF 81. Of these, sixty-one were up-regulated and one hundred two was downregulated in pH 4.8 condition. Also, fourteen spots were only identified in the acid condition, while seven spots was exclusively detected in pH 6.8. Ninety-five differentially expressed spots and two exclusively detected in pH 4,8 were selected for Maldi-Tof identification. Together with the genome sequencing and the proteome analysis of heat stress, we will search for molecular determinants of PRF 81 related to capacity to adapt to stressful tropical conditions.
650 $aNitrogen fixation
650 $aFixação de nitrogênio
700 1 $aPAULITSCH, F.
700 1 $aREICHERT, P. R. S.
700 1 $aKLEPA, M. S.
700 1 $aKLEPA, M. S.
700 1 $aPILEGGI, M.
700 1 $aGOMES, D. F.
700 1 $aHUNGRIA, M.
700 1 $aBATISTA, J. S. S.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 14 | |
| 4. |  | TULLIO, L. D.; PAULITSCH, F.; REICHERT, P. R. S.; KLEPA, M. S.; KLEPA, M. S.; PILEGGI, M.; GOMES, D. F.; HUNGRIA, M.; BATISTA, J. S. S. Proteomic analysis of Rhizobium freirei PRF 81 responses to low pH. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28.; SIMPÓSIO DE FERMENTAÇÃO ALCOÓLICA, 3.; SIMPÓSIO DE MICRORGANISMOS FOTOSSINTETIZANTES, 3.; SIMPÓSIO DE ESCHERICHIA COLI LUIZ RACHID TRABULSI, 4., 2015, Florianópolis. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia, 2015. Res. 1407-2.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 5. | | KLEPA, M. S.; HELENE, L. C. F.; O´HARA, G.; HUNGRIA, M. Bradyrhizobium agreste sp. nov., Bradyrhizobium glycinis sp. nov. and Bradyrhizobium diversitatis sp. nov., isolated from a biodiversity hotspot of the genus Glycine in Western Australia. International Journal of Systematic and Evolutionary Microbiology, v. 71, n. 3, 004742, 2021. 13 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
|  |
| 6. | | HELENE, L. C. F.; KLEPA, M. S.; O'HARA, G.; HUNGRIA, M. Bradyrhizobium archetypum sp. nov., Bradyrhizobium australiense sp. nov. and Bradyrhizobium murdochi sp. nov., isolated from nodules of legumes indigenous to Western Australia International journal of systematic and evolutionary microbiology, v. 70, n. 8, p. 4623-4636, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 7. | | KLEPA, M. S.; HELENE, L. C. F.; O´HARA, G.; HUNGRIA, M. Bradyrhizobium cenepequi sp. nov., Bradyrhizobium semiaridum sp. nov., Bradyrhizobium hereditatis sp. nov. and Bradyrhizobium australafricanum sp. nov., symbionts of different leguminous plants of Western Australia and South Africa and definition of three novel symbiovars. International Journal of Systematic and Evolutionary Microbiology, v. 72, n. 7, 005446, 2022. 18 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
|  |
| 8. | | URQUIAGA, M. C. O.; KLEPA, M. S.; SOMASEGARAN, P.; RIBEIRO, R. A.; DELAMUTA, J. R. M.; HUNGRIA, M. Bradyrhizobium frederickii sp. nov., a nitrogen-fixing lineage isolated from nodules of the caesalpinioid species Chamaecrista fasciculata and characterized by tolerance to high temperature in vitro. International Journal of Systematic and Evolutionary Microbiology, v. 69, n. 12, p. 3863-3877, 2019. 15 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 9. | | KLEPA, M. S.; URQUIAGA, M. C. O.; SOMASEGARAN, P.; DELAMUTA, J. R. M.; RIBEIRO, R. A.; HUNGRIA, M. Bradyrhizobium niftali sp. nov., an effective nitrogen-fixing symbiont of partridge pea [Chamaecrista fasciculata (Michx.) Greene], a native caesalpinioid legume broadly distributed in USA. International Journal of Systematic and Evolutionary Microbiology, v. 69, p. 3448-3459, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 11. | | MOURA, F. T.; HELENE, L. C. F.; KLEPA, M. S.; RIBEIRO, R. A.; NOGUEIRA, M. A.; HUNGRIA, M. Genomes of two type strains of the Rhizobium tropici group: R. calliandrae CCGE524T and R. mayense CCGE526T. Microbiology Resource Announcements, v. 12, n. 9, e00472-23, 2023. 4 p. Os Ts dos códigos são sobrescrito.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Soja. |
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| 12. | | PROTACHEVICZ, A. P.; PAULITSCH, F.; KLEPA, M. S.; HAINOSZ, J.; OLCHANHESKI, L. R.; HUNGRIA, M.; BATISTA, J. S. da S. Pioneering Desmodium spp. are nodulated by natural populations of stress‑tolerant alpha‑ and beta‑rhizobia. Brazilian Journal of Microbiology, v. 54, p. 3127–3135, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 13. | | KLEPA, M. S.; JANONI, V.; PAULITSCH, F.; SILVA, A. R. da; CARMO, M. R. B. do; DELAMUTA, J. R. M.; HUNGRIA, M.; BATISTA, J. S. da S. Molecular diversity of rhizobia-nodulating native Mimosa of Brazilian protected areas. Archives of Microbiology, v. 203, p. 5533-5545, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 14. | | PAULITSCH, F.; KLEPA, M. S.; SILVA, A. R. da; CARMO, M. R. B. do; DALL'AGNOL, R. F.; DELAMUTA, J. R. M.; HUNGRIA, M.; BATISTA, J. S. da S. Phylogenetic diversity of rhizobia nodulating native Mimosa gymnas grown in a South Brazilian ecotone. Molecular Biology Reports, v. 46, p. 529-540, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 14 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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