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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
04/06/1997 |
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Data da última atualização: |
04/06/1997 |
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Autoria: |
DEVAUX, P. KILIAN, A.; KLEINHOFS, A. |
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Afiliação: |
Washigton State University, Pullman-USA. |
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Título: |
Anther culture and Hordeum bulbosum-derived barleydoubled haploids: mutations and methylation. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Mol Gen Genet, v. 241, p. 674-679, 1993. |
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Idioma: |
Inglês |
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Conteúdo: |
Anther culture and Hordeum bulbosum-derived doubled haploid (DH) lines of barley (Hordeum vulgare L.) were analyzed for RFLP and RAPD polymorphisms. Polymorphisms were not detectd in the anther cultureor H. bulbosum-derived DH lines among 273 RFLP and 89 polymerase chain reaction(PCR)-aplified DNA fragments assayed. It was calculated that base substitution or small deletion/insertion mutations had not been induced among 40l 640 bp screened. Large deletion/insertion mutations were not observed among 33 Mb screened. Polymorphisms were observed when DNA wasdigested with the methylation-sensitive restriction enzymes Hpall and MspI: these RFLPs originated primarily from the culture-derived doubled haploids. The data indicate that heritable DNA methylation changeshad occured during DH production, particularly with the anther culture method. |
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Palavras-Chave: |
Doubled haploid; Horedeum bulbosum method; Mutation frequency. |
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Thesaurus Nal: |
anther culture; DNA methylation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01413naa a2200193 a 4500
001 1647895
005 1997-06-04
008 1993 bl --- 0-- u #d
100 1 $aDEVAUX, P. KILIAN, A.
245 $aAnther culture and Hordeum bulbosum-derived barleydoubled haploids$bmutations and methylation.
260 $c1993
520 $aAnther culture and Hordeum bulbosum-derived doubled haploid (DH) lines of barley (Hordeum vulgare L.) were analyzed for RFLP and RAPD polymorphisms. Polymorphisms were not detectd in the anther cultureor H. bulbosum-derived DH lines among 273 RFLP and 89 polymerase chain reaction(PCR)-aplified DNA fragments assayed. It was calculated that base substitution or small deletion/insertion mutations had not been induced among 40l 640 bp screened. Large deletion/insertion mutations were not observed among 33 Mb screened. Polymorphisms were observed when DNA wasdigested with the methylation-sensitive restriction enzymes Hpall and MspI: these RFLPs originated primarily from the culture-derived doubled haploids. The data indicate that heritable DNA methylation changeshad occured during DH production, particularly with the anther culture method.
650 $aanther culture
650 $aDNA methylation
653 $aDoubled haploid
653 $aHoredeum bulbosum method
653 $aMutation frequency
700 1 $aKLEINHOFS, A.
773 $tMol Gen Genet$gv. 241, p. 674-679, 1993.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
