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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
24/09/2018 |
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Data da última atualização: |
24/09/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA FILHO, J. X. de; MORES, M. A. Z.; REBELATTO, R.; KICH, J. D.; CANTAO, M. E.; KLEIN, C. S.; GUEDES, R. M. C.; COLDEBELLA, A.; BARCELLOS, D. E. S. N. de; MORES, N. |
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Afiliação: |
JOÃO XAVIER DE OLIVEIRA FILHO; MARCOS ANTONIO ZANELLA MORES, CNPSA; RAQUEL REBELATTO, CNPSA; JALUSA DEON KICH, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; CATIA SILENE KLEIN, CNPSA; ROBERTO MAURÍCIO CARVALHO GUEDES; ARLEI COLDEBELLA, CNPSA; DAVID EMÍLIO SANTOS NEVES de BARCELLOS; NELSON MORES, CNPSA. |
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Título: |
Pathogenic variability among Pasteurella multocida type A isolates from Brazilian pig farms. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
BMC Veterinary Research, v. 14, n. 244, 2018. |
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DOI: |
10.1186/s12917-018-1565-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Background: Pasteurella multocida type A (PmA) is considered a secondary agent of pneumonia in pigs. The role of PmA as a primary pathogen was investigated by challenging pigs with eight field strains isolated from pneumonia and serositis in six Brazilian states. Eight groups of eight pigs each were intranasally inoculated with different strains of PmA (1.5 mL/nostril of 10e7 CFU/mL). The control group (n = 12) received sterile PBS. The pigs were euthanized by electrocution and necropsied by 5 dpi. Macroscopic lesions were recorded, and swabs and fragments of thoracic and abdominal organs were analyzed by bacteriological and pathological assays. The PmA strains were analyzed for four virulence genes (toxA: toxin; pfhA: adhesion; tbpA and hgbB: iron acquisition) by PCR and sequencing and submitted to multilocus sequence typing (MLST). Results: The eight PmA strains were classified as follows: five as highly pathogenic (HP) for causing necrotic bronchopneumonia and diffuse fibrinous pleuritis and pericarditis; one as low pathogenic for causing only focal bronchopneumonia; and two as nonpathogenic because they did not cause injury to any pig. PCR for the gene pfhA was positive for all five HP isolates. Sequencing demonstrated that the pfhA region of the HP strains comprised four genes: tpsB1, pfhA1, tpsB2 and pfhA2. The low and nonpathogenic strains did not contain the genes tpsB2 and pfhA2. A deletion of four bases was observed in the pfhA gene in the low pathogenic strain, and an insertion of 37 kb of phage DNA was observed in the nonpathogenic strains. MLST clustered the HP isolates in one group and the low and nonpathogenic isolates in another. Only the nonpathogenic isolates matched sequence type 10; the other isolates did not match any type available in the MLST database. Conclusions: The hypothesis that some PmA strains are primary pathogens and cause disease in pigs without any co-factor was confirmed. The pfhA region, comprising the genes tpsB1, tpsB2, pfhA1 and pfhA2, is related to the pathogenicity of PmA. The HP strains can cause necrotic bronchopneumonia, fibrinous pleuritis and pericarditis in pigs and can be identified by PCR amplification of the gene pfhA2. MenosAbstract: Background: Pasteurella multocida type A (PmA) is considered a secondary agent of pneumonia in pigs. The role of PmA as a primary pathogen was investigated by challenging pigs with eight field strains isolated from pneumonia and serositis in six Brazilian states. Eight groups of eight pigs each were intranasally inoculated with different strains of PmA (1.5 mL/nostril of 10e7 CFU/mL). The control group (n = 12) received sterile PBS. The pigs were euthanized by electrocution and necropsied by 5 dpi. Macroscopic lesions were recorded, and swabs and fragments of thoracic and abdominal organs were analyzed by bacteriological and pathological assays. The PmA strains were analyzed for four virulence genes (toxA: toxin; pfhA: adhesion; tbpA and hgbB: iron acquisition) by PCR and sequencing and submitted to multilocus sequence typing (MLST). Results: The eight PmA strains were classified as follows: five as highly pathogenic (HP) for causing necrotic bronchopneumonia and diffuse fibrinous pleuritis and pericarditis; one as low pathogenic for causing only focal bronchopneumonia; and two as nonpathogenic because they did not cause injury to any pig. PCR for the gene pfhA was positive for all five HP isolates. Sequencing demonstrated that the pfhA region of the HP strains comprised four genes: tpsB1, pfhA1, tpsB2 and pfhA2. The low and nonpathogenic strains did not contain the genes tpsB2 and pfhA2. A deletion of four bases was observed in the pfhA gene in the low pathogenic ... Mostrar Tudo |
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Palavras-Chave: |
Broncopneumonia; PCR; PfhA; Poliserositis; Polyserositis. |
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Thesagro: |
Doença Respiratória; Suíno. |
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Thesaurus Nal: |
Bronchopneumonia; Multilocus sequence typing; Pathogenicity; Polymerase chain reaction; Respiratory tract diseases; Swine; Virulent strains. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03381naa a2200409 a 4500
001 2096196
005 2018-09-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1186/s12917-018-1565-2$2DOI
100 1 $aOLIVEIRA FILHO, J. X. de
245 $aPathogenic variability among Pasteurella multocida type A isolates from Brazilian pig farms.$h[electronic resource]
260 $c2018
520 $aAbstract: Background: Pasteurella multocida type A (PmA) is considered a secondary agent of pneumonia in pigs. The role of PmA as a primary pathogen was investigated by challenging pigs with eight field strains isolated from pneumonia and serositis in six Brazilian states. Eight groups of eight pigs each were intranasally inoculated with different strains of PmA (1.5 mL/nostril of 10e7 CFU/mL). The control group (n = 12) received sterile PBS. The pigs were euthanized by electrocution and necropsied by 5 dpi. Macroscopic lesions were recorded, and swabs and fragments of thoracic and abdominal organs were analyzed by bacteriological and pathological assays. The PmA strains were analyzed for four virulence genes (toxA: toxin; pfhA: adhesion; tbpA and hgbB: iron acquisition) by PCR and sequencing and submitted to multilocus sequence typing (MLST). Results: The eight PmA strains were classified as follows: five as highly pathogenic (HP) for causing necrotic bronchopneumonia and diffuse fibrinous pleuritis and pericarditis; one as low pathogenic for causing only focal bronchopneumonia; and two as nonpathogenic because they did not cause injury to any pig. PCR for the gene pfhA was positive for all five HP isolates. Sequencing demonstrated that the pfhA region of the HP strains comprised four genes: tpsB1, pfhA1, tpsB2 and pfhA2. The low and nonpathogenic strains did not contain the genes tpsB2 and pfhA2. A deletion of four bases was observed in the pfhA gene in the low pathogenic strain, and an insertion of 37 kb of phage DNA was observed in the nonpathogenic strains. MLST clustered the HP isolates in one group and the low and nonpathogenic isolates in another. Only the nonpathogenic isolates matched sequence type 10; the other isolates did not match any type available in the MLST database. Conclusions: The hypothesis that some PmA strains are primary pathogens and cause disease in pigs without any co-factor was confirmed. The pfhA region, comprising the genes tpsB1, tpsB2, pfhA1 and pfhA2, is related to the pathogenicity of PmA. The HP strains can cause necrotic bronchopneumonia, fibrinous pleuritis and pericarditis in pigs and can be identified by PCR amplification of the gene pfhA2.
650 $aBronchopneumonia
650 $aMultilocus sequence typing
650 $aPathogenicity
650 $aPolymerase chain reaction
650 $aRespiratory tract diseases
650 $aSwine
650 $aVirulent strains
650 $aDoença Respiratória
650 $aSuíno
653 $aBroncopneumonia
653 $aPCR
653 $aPfhA
653 $aPoliserositis
653 $aPolyserositis
700 1 $aMORES, M. A. Z.
700 1 $aREBELATTO, R.
700 1 $aKICH, J. D.
700 1 $aCANTAO, M. E.
700 1 $aKLEIN, C. S.
700 1 $aGUEDES, R. M. C.
700 1 $aCOLDEBELLA, A.
700 1 $aBARCELLOS, D. E. S. N. de
700 1 $aMORES, N.
773 $tBMC Veterinary Research$gv. 14, n. 244, 2018.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
05/09/2018 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
TREVIZAN, J. T.; NOCITI, R. P.; PEDROSA, A. C.; BRAIR, V. L.; OLIVEIRA, I. C.; BALARO, M. F. A.; MAIA, A. L. R. S.; PRASTES, J. F.; BRANDÃO, F. Z.; FONSECA, J. F. da; OLIVEIRA, M. E. |
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Afiliação: |
Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias (Unesp/FCAV) - Jaboticabal, SP, Brazil; Centro Universitário de Rio Preto (UNIRP) - São José do Rio Preto, SP, Brazil; Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; Jeferson Ferreira da Fonseca, CNPC. |
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Título: |
Use of hCG, eCG or p-FSH on estrus induction of goats and their effects on luteal dynamics and conception rate. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Animal Reprodroduction, v. 15, n. 3, p. 324, Jul./Sept. 2018. Abstract A009. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
The present study tested the hypothesis that hCG and pFSH stimulate the follicular development and consequently corpora lutea, in a manner equivalent to those induced by eCG in goat. Eighty-five Toggenburg goats, during the seasonal transition period (December, 21ºS), underwent a short-duration estrus induction/synchronization protocol with a sponge impregnated with medroxyprogesterone acetate (60 mg MAP, 6 days). In conclusion, the use of hCG and pFSH in protocol of estrus induction/synchronization in goats induces equivalent luteal dynamics and conception rate, being substitutes for eCG. |
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Palavras-Chave: |
Corpora lutea; ECG; HCG; Induction. |
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Thesagro: |
Caprino; Ciclo Estral; Corpo Lúteo; Prenhez; Reprodução Animal; Sincronização do Cio; Transferência de Embrião. |
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Thesaurus NAL: |
Animal reproduction; Artificial insemination; Embryo transfer; Estrus synchronization; Follicular development; Goats; Medroxyprogesterone; Pregnancy. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182497/1/CNPC-2018-Use-of-hCG.pdf
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Marc: |
LEADER 02077nam a2200457 a 4500
001 2095126
005 2019-09-23
008 2018 bl uuuu u00u1 u #d
100 1 $aTREVIZAN, J. T.
245 $aUse of hCG, eCG or p-FSH on estrus induction of goats and their effects on luteal dynamics and conception rate.$h[electronic resource]
260 $aAnimal Reprodroduction, v. 15, n. 3, p. 324, Jul./Sept. 2018. Abstract A009. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th$c2018
520 $aThe present study tested the hypothesis that hCG and pFSH stimulate the follicular development and consequently corpora lutea, in a manner equivalent to those induced by eCG in goat. Eighty-five Toggenburg goats, during the seasonal transition period (December, 21ºS), underwent a short-duration estrus induction/synchronization protocol with a sponge impregnated with medroxyprogesterone acetate (60 mg MAP, 6 days). In conclusion, the use of hCG and pFSH in protocol of estrus induction/synchronization in goats induces equivalent luteal dynamics and conception rate, being substitutes for eCG.
650 $aAnimal reproduction
650 $aArtificial insemination
650 $aEmbryo transfer
650 $aEstrus synchronization
650 $aFollicular development
650 $aGoats
650 $aMedroxyprogesterone
650 $aPregnancy
650 $aCaprino
650 $aCiclo Estral
650 $aCorpo Lúteo
650 $aPrenhez
650 $aReprodução Animal
650 $aSincronização do Cio
650 $aTransferência de Embrião
653 $aCorpora lutea
653 $aECG
653 $aHCG
653 $aInduction
700 1 $aNOCITI, R. P.
700 1 $aPEDROSA, A. C.
700 1 $aBRAIR, V. L.
700 1 $aOLIVEIRA, I. C.
700 1 $aBALARO, M. F. A.
700 1 $aMAIA, A. L. R. S.
700 1 $aPRASTES, J. F.
700 1 $aBRANDÃO, F. Z.
700 1 $aFONSECA, J. F. da
700 1 $aOLIVEIRA, M. E.
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