Data/hora: 13/06/2024 08:57:00

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 04/06/1997
Data da última atualização: | 04/06/1997
Autoria: | DEVAUX, P. KILIAN, A.; KLEINHOFS, A.
Afiliação: | Washigton State University, Pullman-USA.
Título: | Anther culture and Hordeum bulbosum-derived barleydoubled haploids: mutations and methylation.
Ano de publicação: | 1993
Fonte/Imprenta: | Mol Gen Genet, v. 241, p. 674-679, 1993.
Idioma: | Inglês
Conteúdo: | Anther culture and Hordeum bulbosum-derived doubled haploid (DH) lines of barley (Hordeum vulgare L.) were analyzed for RFLP and RAPD polymorphisms. Polymorphisms were not detectd in the anther cultureor H. bulbosum-derived DH lines among 273 RFLP and 89 polymerase chain reaction(PCR)-aplified DNA fragments assayed. It was calculated that base substitution or small deletion/insertion mutations had not been induced among 40l 640 bp screened. Large deletion/insertion mutations were not observed among 33 Mb screened. Polymorphisms were observed when DNA wasdigested with the methylation-sensitive restriction enzymes Hpall and MspI: these RFLPs originated primarily from the culture-derived doubled haploids. The data indicate that heritable DNA methylation changeshad occured during DH production, particularly with the anther culture method.
Palavras-Chave: | Doubled haploid; Horedeum bulbosum method; Mutation frequency.
Thesaurus Nal: | anther culture; DNA methylation.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (16926-1) - ADC - SP - -- - S11042 -  - S11042 - DIS