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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
06/07/1998 |
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Data da última atualização: |
28/03/2017 |
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Autoria: |
RICE; C. D.; KERGOSIEN, D. H.; ADAMS, S. M. |
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Título: |
Innate immune function as a bioindicator of pollution stress in fish. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Ecotoxicology and Environmental Safety, v.33, n.2, p.186-192, 1996. |
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Idioma: |
Inglês |
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Conteúdo: |
Immunotoxicological studies, bnased on processing of samples in the field and laboratory, were conducted on fish collected from a stream receiving point-source contaminants near its headwaters. Previous studies in this stream have revealed that cytochrome P4501A activity, liver somatic indices, macrophage aggregates, and parasitic liver lesion are significantly elevated in sunfish with the degree of impact decreasing with distance from the contaminant source. Fish collected from each sampling site were equally divided, One group was sacrificed in the field and the spleen and anterior kidney tissues were removed and placed in buffer on ice. The other group was kept in MS-222 fopr 2 hr and transported to the laboratory for processing. The spleen and anterior kidney from each fish were then prepared as a single cell suspension and shipped overnight to Mississippi State University. Cells were then evaluated for PMA-stimulated phagocyte oxidative burst and non-specific cytotoxic cell (NCC) activity against K562 tumor targets. Oxidative burst responses were drmatically suppressed in both groups at sampling sites near the headwaters but returned to reference further downstream. There were no differences between processing strategies at each station. NCC activities did not follow gradient-response patterns observed with phagocyte oxidative burst data and there were inconsistent differences between processing strategies at each site. These data indicate that simple immune function assays, such as phagocyte oxidative burst responses, can be used as a ancillary bioindicator in fish health monitoring and that immune function in these fish can be reliably assessed even if samples are not immediately processed. MenosImmunotoxicological studies, bnased on processing of samples in the field and laboratory, were conducted on fish collected from a stream receiving point-source contaminants near its headwaters. Previous studies in this stream have revealed that cytochrome P4501A activity, liver somatic indices, macrophage aggregates, and parasitic liver lesion are significantly elevated in sunfish with the degree of impact decreasing with distance from the contaminant source. Fish collected from each sampling site were equally divided, One group was sacrificed in the field and the spleen and anterior kidney tissues were removed and placed in buffer on ice. The other group was kept in MS-222 fopr 2 hr and transported to the laboratory for processing. The spleen and anterior kidney from each fish were then prepared as a single cell suspension and shipped overnight to Mississippi State University. Cells were then evaluated for PMA-stimulated phagocyte oxidative burst and non-specific cytotoxic cell (NCC) activity against K562 tumor targets. Oxidative burst responses were drmatically suppressed in both groups at sampling sites near the headwaters but returned to reference further downstream. There were no differences between processing strategies at each station. NCC activities did not follow gradient-response patterns observed with phagocyte oxidative burst data and there were inconsistent differences between processing strategies at each site. These data indicate that simple immune function a... Mostrar Tudo |
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Palavras-Chave: |
Bioindicador; Bioindicator; Funcao imunologica; Immune function. |
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Thesagro: |
Peixe; Poluição. |
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Thesaurus Nal: |
fish; pollution. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02383naa a2200253 a 4500
001 1792737
005 2017-03-28
008 1996 bl --- 0-- u #d
100 1 $aRICE
245 $aInnate immune function as a bioindicator of pollution stress in fish.
260 $c1996
520 $aImmunotoxicological studies, bnased on processing of samples in the field and laboratory, were conducted on fish collected from a stream receiving point-source contaminants near its headwaters. Previous studies in this stream have revealed that cytochrome P4501A activity, liver somatic indices, macrophage aggregates, and parasitic liver lesion are significantly elevated in sunfish with the degree of impact decreasing with distance from the contaminant source. Fish collected from each sampling site were equally divided, One group was sacrificed in the field and the spleen and anterior kidney tissues were removed and placed in buffer on ice. The other group was kept in MS-222 fopr 2 hr and transported to the laboratory for processing. The spleen and anterior kidney from each fish were then prepared as a single cell suspension and shipped overnight to Mississippi State University. Cells were then evaluated for PMA-stimulated phagocyte oxidative burst and non-specific cytotoxic cell (NCC) activity against K562 tumor targets. Oxidative burst responses were drmatically suppressed in both groups at sampling sites near the headwaters but returned to reference further downstream. There were no differences between processing strategies at each station. NCC activities did not follow gradient-response patterns observed with phagocyte oxidative burst data and there were inconsistent differences between processing strategies at each site. These data indicate that simple immune function assays, such as phagocyte oxidative burst responses, can be used as a ancillary bioindicator in fish health monitoring and that immune function in these fish can be reliably assessed even if samples are not immediately processed.
650 $afish
650 $apollution
650 $aPeixe
650 $aPoluição
653 $aBioindicador
653 $aBioindicator
653 $aFuncao imunologica
653 $aImmune function
700 1 $aC. D.
700 1 $aKERGOSIEN, D. H.
700 1 $aADAMS, S. M.
773 $tEcotoxicology and Environmental Safety$gv.33, n.2, p.186-192, 1996.
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Registro original: |
Embrapa Pantanal (CPAP) |
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