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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Ocidental. Para informações adicionais entre em contato com cpaa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
23/01/2013 |
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Data da última atualização: |
25/03/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MUNIZ, C. R.; FREIRE, F. das C. O.; VIANA, F. M. P.; CARDOSO, J. E.; CORREIA, D.; JALINK, H.; KEMA, G. H. J.; SILVA, G. F. da; GUEDES, M. I. F. |
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Afiliação: |
CELLI RODRIGUES MUNIZ, CNPAT; FRANCISCO DAS CHAGAS O FREIRE, CNPAT; FRANCISCO MARTO PINTO VIANA, CNPAT; JOSE EMILSON CARDOSO, CNPAT; DIVA CORREIA, CNPAT; WAGENINGEN UNIVERSITY GREENHOUSE HORTICULTURE; PLANT RESEARCH INTERNATIONAL; GILVAN FERREIRA DA SILVA, CPAA; UNIVERSIDADE ESTADUAL DO CEARÁ. |
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Título: |
Polyclonal antibody-based ELISA in combination with specific PCR amplification of internal transcribed spacer regions for the detection and quantitation of Lasiodiplodia theobromae, causal agent of gummosis in cashew nut plants. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Annals of Applied Biology, v. 160, n. 3, p. 217-224, 2012. |
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ISSN: |
0003-4746 |
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DOI: |
10.1111/j.1744-7348.2012.00534.x |
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Idioma: |
Português |
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Conteúdo: |
Members of Botryosphaeriaceae family are associated with serious diseases in different plants across the world. In cashew nut plants (Anacardium occidentale), the fungus Lasiodiplodia theobromae causes a severe group of symptoms related to gummosis that results in decreased nut production. The aim of this work was to develop an indirect enzyme-linked immunosorbent assay (ELISA) with sufficient sensitivity and specificity to detect the fungus both in vitro and in planta (artificially and naturally infected) and to increase the detection specificity within the fungi group using primers specific for the internal transcribed spacer (ITS) sequences. A collection of L. theobromae isolates was obtained, and antisera against the fungus were raised in rabbits. Cross-reactivity against Neofusicoccum sp., Colletotrichum gloeosporioides, Phomopsis anacardii and Pestalotiopsis guepinii was examined. Naturally and artificially infected vegetal material were employed in the ELISAs. The fungi ITS sequences were determined, and single nucleotide polymorphisms were identified and used for primer design. For the naturally infected plants, there was an approximately fourfold variation in the absorbance values. Some positive readings for asymptomatic samples were detected. For the artificially infected samples, an ELISA-based weekly timecourse analysis was conducted, and the values for samples from 0 and 7 days were lower than the threshold value. Beginning on day 14, the infection could be detected, with rates varying from 40% on day 14 to 80% on day 21 and 100% by the end of the experiment. The ITS sequencing revealed few polymorphisms among the L. theobromae isolates, but for C. gloeosporioides, P. anacardii, P. guepinii and Neofusicoccum sp., the sequences were sufficient to permit reliable discrimination. The feasibility of ELISA as an early detection technique to assist in gummosis management was demonstrated. PCR amplification based on ITS regions increases and complements serological specificity. MenosMembers of Botryosphaeriaceae family are associated with serious diseases in different plants across the world. In cashew nut plants (Anacardium occidentale), the fungus Lasiodiplodia theobromae causes a severe group of symptoms related to gummosis that results in decreased nut production. The aim of this work was to develop an indirect enzyme-linked immunosorbent assay (ELISA) with sufficient sensitivity and specificity to detect the fungus both in vitro and in planta (artificially and naturally infected) and to increase the detection specificity within the fungi group using primers specific for the internal transcribed spacer (ITS) sequences. A collection of L. theobromae isolates was obtained, and antisera against the fungus were raised in rabbits. Cross-reactivity against Neofusicoccum sp., Colletotrichum gloeosporioides, Phomopsis anacardii and Pestalotiopsis guepinii was examined. Naturally and artificially infected vegetal material were employed in the ELISAs. The fungi ITS sequences were determined, and single nucleotide polymorphisms were identified and used for primer design. For the naturally infected plants, there was an approximately fourfold variation in the absorbance values. Some positive readings for asymptomatic samples were detected. For the artificially infected samples, an ELISA-based weekly timecourse analysis was conducted, and the values for samples from 0 and 7 days were lower than the threshold value. Beginning on day 14, the infection could be dete... Mostrar Tudo |
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Palavras-Chave: |
Anacardium occidentale L; Cashew agri-industry; Endophytic fungi; Immunodetection. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03001naa a2200289 a 4500
001 1946100
005 2015-03-25
008 2012 bl uuuu u00u1 u #d
022 $a0003-4746
024 7 $a10.1111/j.1744-7348.2012.00534.x$2DOI
100 1 $aMUNIZ, C. R.
245 $aPolyclonal antibody-based ELISA in combination with specific PCR amplification of internal transcribed spacer regions for the detection and quantitation of Lasiodiplodia theobromae, causal agent of gummosis in cashew nut plants.
260 $c2012
520 $aMembers of Botryosphaeriaceae family are associated with serious diseases in different plants across the world. In cashew nut plants (Anacardium occidentale), the fungus Lasiodiplodia theobromae causes a severe group of symptoms related to gummosis that results in decreased nut production. The aim of this work was to develop an indirect enzyme-linked immunosorbent assay (ELISA) with sufficient sensitivity and specificity to detect the fungus both in vitro and in planta (artificially and naturally infected) and to increase the detection specificity within the fungi group using primers specific for the internal transcribed spacer (ITS) sequences. A collection of L. theobromae isolates was obtained, and antisera against the fungus were raised in rabbits. Cross-reactivity against Neofusicoccum sp., Colletotrichum gloeosporioides, Phomopsis anacardii and Pestalotiopsis guepinii was examined. Naturally and artificially infected vegetal material were employed in the ELISAs. The fungi ITS sequences were determined, and single nucleotide polymorphisms were identified and used for primer design. For the naturally infected plants, there was an approximately fourfold variation in the absorbance values. Some positive readings for asymptomatic samples were detected. For the artificially infected samples, an ELISA-based weekly timecourse analysis was conducted, and the values for samples from 0 and 7 days were lower than the threshold value. Beginning on day 14, the infection could be detected, with rates varying from 40% on day 14 to 80% on day 21 and 100% by the end of the experiment. The ITS sequencing revealed few polymorphisms among the L. theobromae isolates, but for C. gloeosporioides, P. anacardii, P. guepinii and Neofusicoccum sp., the sequences were sufficient to permit reliable discrimination. The feasibility of ELISA as an early detection technique to assist in gummosis management was demonstrated. PCR amplification based on ITS regions increases and complements serological specificity.
653 $aAnacardium occidentale L
653 $aCashew agri-industry
653 $aEndophytic fungi
653 $aImmunodetection
700 1 $aFREIRE, F. das C. O.
700 1 $aVIANA, F. M. P.
700 1 $aCARDOSO, J. E.
700 1 $aCORREIA, D.
700 1 $aJALINK, H.
700 1 $aKEMA, G. H. J.
700 1 $aSILVA, G. F. da
700 1 $aGUEDES, M. I. F.
773 $tAnnals of Applied Biology$gv. 160, n. 3, p. 217-224, 2012.
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Embrapa Amazônia Ocidental (CPAA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
30/12/2016 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
SEPÚLVEDA, R V.; VALENTE, F. L.; REIS, E. C. C.; ARAÚJO, F. R.; ELEOTÉRIO R. B.; QUEIROZ, P. V. S.; BORGES, A. P. B. |
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Afiliação: |
RODRIGO V. SEPÚLVEDA, UFV; FABRÍCIO L. VALENTE, UFV; EMILY C. C. REIS, UFV; FABIANA R ARAÚJO, UFV; RENATO B. ELEOTÉRIO, UFV; PAULO V. S. QUEIROZ, UFV; ANDRÉA P. B. BORGES, UFV. |
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Título: |
Bacterial cellulose and bacterial cellulose/polycaprolactone composites as tissue substitutes in rabbits' cornea. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 10, p. 986-992, out. 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
In order to test the performance of bacterial cellulose/polycaprolactone composite (BC/PCL) and pure bacterial cellulose (BC) as tissue substitutes in rabbits? cornea, a superficial ulcer containing 5mm in diameter and 0.2mm deep was made in the right cornea of 36 rabbits, then a interlayer pocket was created from the basis of this ulcer. Twelve rabbits received BC/PCL membrane and 12 were treated with BC membranes, both membranes with 8mm in diameter. The remaining rabbits received no membrane constituting the control group. The animals were clinically followed up for 45 days. Three animals of each group were euthanized at three, seven, 21, and 45 days after implantation for histological examination of the cornea along with the implant. Clinical observation revealed signs of moderate inflammatory process, decreasing from day 20th in the implanted groups. Histology showed absence of epithelium on the membranes, fibroplasia close to the implants, lymph inflammatory infiltrate with giant cells, collagen disorganization, with a predominance of immature collagen fibers in both groups with implants. Although inflammatory response is acceptable, the membranes used does not satisfactorily played the role of tissue substitute for the cornea during the study period. |
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Palavras-Chave: |
Bacterial cellulose; Bacterial cellulose/polycaprolactone composite; Biomaterial; Celulose bacteriana; Corneal ulcer; Engenharia de tecido; Modelo animal; Tissue substitutes; Úlcera de córnea. |
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Thesagro: |
Coelho. |
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Thesaurus NAL: |
Animal models; Biocompatible materials; Cornea; Gluconacetobacter xylinus; Rabbits; Tissue engineering. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/152581/1/Bacterial-cellulose-and-bacterial.pdf
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Marc: |
LEADER 02466naa a2200385 a 4500
001 2059677
005 2017-12-29
008 2016 bl uuuu u00u1 u #d
100 1 $aSEPÚLVEDA, R V.
245 $aBacterial cellulose and bacterial cellulose/polycaprolactone composites as tissue substitutes in rabbits' cornea.$h[electronic resource]
260 $c2016
520 $aIn order to test the performance of bacterial cellulose/polycaprolactone composite (BC/PCL) and pure bacterial cellulose (BC) as tissue substitutes in rabbits? cornea, a superficial ulcer containing 5mm in diameter and 0.2mm deep was made in the right cornea of 36 rabbits, then a interlayer pocket was created from the basis of this ulcer. Twelve rabbits received BC/PCL membrane and 12 were treated with BC membranes, both membranes with 8mm in diameter. The remaining rabbits received no membrane constituting the control group. The animals were clinically followed up for 45 days. Three animals of each group were euthanized at three, seven, 21, and 45 days after implantation for histological examination of the cornea along with the implant. Clinical observation revealed signs of moderate inflammatory process, decreasing from day 20th in the implanted groups. Histology showed absence of epithelium on the membranes, fibroplasia close to the implants, lymph inflammatory infiltrate with giant cells, collagen disorganization, with a predominance of immature collagen fibers in both groups with implants. Although inflammatory response is acceptable, the membranes used does not satisfactorily played the role of tissue substitute for the cornea during the study period.
650 $aAnimal models
650 $aBiocompatible materials
650 $aCornea
650 $aGluconacetobacter xylinus
650 $aRabbits
650 $aTissue engineering
650 $aCoelho
653 $aBacterial cellulose
653 $aBacterial cellulose/polycaprolactone composite
653 $aBiomaterial
653 $aCelulose bacteriana
653 $aCorneal ulcer
653 $aEngenharia de tecido
653 $aModelo animal
653 $aTissue substitutes
653 $aÚlcera de córnea
700 1 $aVALENTE, F. L.
700 1 $aREIS, E. C. C.
700 1 $aARAÚJO, F. R.
700 1 $aELEOTÉRIO R. B.
700 1 $aQUEIROZ, P. V. S.
700 1 $aBORGES, A. P. B.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 10, p. 986-992, out. 2016.
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