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Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Ocidental. |
Data corrente: |
10/01/2012 |
Data da última atualização: |
22/04/2021 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
LOBO, I. K. C.; HANADA, R. E.; SOUSA, N. R.; GASPAROTTO, L.; SOUZA Jr. M. T.; KEMA, G. H. J.; SILVA, G. F. da. |
Afiliação: |
I. K. C. Lobo; R. E. Hanada, INPA; NELCIMAR REIS SOUSA, CPAA; LUADIR GASPAROTTO, CPAA; EMBRAPA AGROENERGIA; GILVAN FERREIRA DA SILVA, CPAA. |
Título: |
Construction of gateway binary vector for selection with bialaphos or carboxin and GFP expression in fungi. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE GENÉTICA, 57., 2011, Águas de Lindóia. Resumos... s.l.: SBG, 2011. p. 25. |
Idioma: |
Português |
Conteúdo: |
Genomic data has created a growing demand for tools and methodologies for studying the genes function, which can be realized through loss of function experiments (gene knockout) or by RNA silencing (knockdown). The develop-ment of binary vectors for Agrobacterium tumefaciens mediated transformation (ATMT) has the advantage of being independent of protoplast formation and can be used directly on a wide variety of fungal species and tissue types. The selection of transformants using bialaphos and carboxin has the advantages of low cost in the transformation and availability of different selectable markers, also allowing the analysis of several genes and combination of study by knockout or knockdown, using selectable markers in the same transformant. Thus, this study aimed to build two binary vectors containing reporter gene and selectable markers that confer resistance to carboxin and bialaphos. The cassettes were constructed using the Gateway system to two fragments. The gene encoding the GFP protein and PtoxA and PtrpCpromoters were cloned into pDONR P1-P5R plasmid. Genes that confer bialaphos and carboxin resistance, bar and cbxr respectively, were cloned into pDONR P5-P2 plasmid. The pPGW plasmid was used as des-tination vector. The gfp gene transcription is controlled by PtoxA promoter and the bar and cbxr genes transcriptions are controlled by PtrpC promoter. These binary vectors were named pGWGFP-BAR and pGWGFP-CBXR. The assembly of cassettes was confi rmed by sequencing, and the validation of vectors is being accomplished through transformation (ATMT) with the plant pathogens Mycosphaerella fi jiensis and Fusarium oxysporum f. sp. cubense. MenosGenomic data has created a growing demand for tools and methodologies for studying the genes function, which can be realized through loss of function experiments (gene knockout) or by RNA silencing (knockdown). The develop-ment of binary vectors for Agrobacterium tumefaciens mediated transformation (ATMT) has the advantage of being independent of protoplast formation and can be used directly on a wide variety of fungal species and tissue types. The selection of transformants using bialaphos and carboxin has the advantages of low cost in the transformation and availability of different selectable markers, also allowing the analysis of several genes and combination of study by knockout or knockdown, using selectable markers in the same transformant. Thus, this study aimed to build two binary vectors containing reporter gene and selectable markers that confer resistance to carboxin and bialaphos. The cassettes were constructed using the Gateway system to two fragments. The gene encoding the GFP protein and PtoxA and PtrpCpromoters were cloned into pDONR P1-P5R plasmid. Genes that confer bialaphos and carboxin resistance, bar and cbxr respectively, were cloned into pDONR P5-P2 plasmid. The pPGW plasmid was used as des-tination vector. The gfp gene transcription is controlled by PtoxA promoter and the bar and cbxr genes transcriptions are controlled by PtrpC promoter. These binary vectors were named pGWGFP-BAR and pGWGFP-CBXR. The assembly of cassettes was confi rmed by sequencin... Mostrar Tudo |
Thesagro: |
Genética. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/51989/1/Resumo-Igor-co-autor.pdf
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Marc: |
LEADER 02305nam a2200193 a 4500 001 1912252 005 2021-04-22 008 2011 bl uuuu u00u1 u #d 100 1 $aLOBO, I. K. C. 245 $aConstruction of gateway binary vector for selection with bialaphos or carboxin and GFP expression in fungi. 260 $aIn: CONGRESSO BRASILEIRO DE GENÉTICA, 57., 2011, Águas de Lindóia. Resumos... s.l.: SBG, 2011. p. 25.$c2011 520 $aGenomic data has created a growing demand for tools and methodologies for studying the genes function, which can be realized through loss of function experiments (gene knockout) or by RNA silencing (knockdown). The develop-ment of binary vectors for Agrobacterium tumefaciens mediated transformation (ATMT) has the advantage of being independent of protoplast formation and can be used directly on a wide variety of fungal species and tissue types. The selection of transformants using bialaphos and carboxin has the advantages of low cost in the transformation and availability of different selectable markers, also allowing the analysis of several genes and combination of study by knockout or knockdown, using selectable markers in the same transformant. Thus, this study aimed to build two binary vectors containing reporter gene and selectable markers that confer resistance to carboxin and bialaphos. The cassettes were constructed using the Gateway system to two fragments. The gene encoding the GFP protein and PtoxA and PtrpCpromoters were cloned into pDONR P1-P5R plasmid. Genes that confer bialaphos and carboxin resistance, bar and cbxr respectively, were cloned into pDONR P5-P2 plasmid. The pPGW plasmid was used as des-tination vector. The gfp gene transcription is controlled by PtoxA promoter and the bar and cbxr genes transcriptions are controlled by PtrpC promoter. These binary vectors were named pGWGFP-BAR and pGWGFP-CBXR. The assembly of cassettes was confi rmed by sequencing, and the validation of vectors is being accomplished through transformation (ATMT) with the plant pathogens Mycosphaerella fi jiensis and Fusarium oxysporum f. sp. cubense. 650 $aGenética 700 1 $aHANADA, R. E. 700 1 $aSOUSA, N. R. 700 1 $aGASPAROTTO, L. 700 1 $aSOUZA Jr. M. T. 700 1 $aKEMA, G. H. J. 700 1 $aSILVA, G. F. da
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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Registros recuperados : 42 | |
1. | | DITA, M. A.; KOBAYASHI, A. K.; WAALWIJK, C.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Genetic transformation of Fusarium oxysporum f. sp. cubense. Tropical Plant Pathology, v. 34, p. S177, ago. 2009. Suplemento. Ref. 606. Edição dos Resumos do 42° Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio-Norte. |
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3. | | KOBAYASHI, A. K.; RODRIGUEZ, M. A. D.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Generation of Fusarium f. sp. passiflorae strains expressing reporter genes. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 607.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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7. | | KOBAYASHI, A. K.; DITA, M. A.; TROCOLI, R. O.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. GFP and RFP transformation of Fusarium guttiforme (sin. Fusarium subglutinans f. sp. annanas). Tropical Plant Pathology, v. 34, p. S156, ago. 2009. Suplemento. Ref. 545. Edição dos Resumos do 42° Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio-Norte. |
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8. | | RODRIGUEZ, M. A. D.; KOBAYASHI, A. K.; WAALWIJK, C.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Genetic transformation of Fusarium oxysporum f. sp. cubense. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 606.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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9. | | RODRIGUEZ, M. A. D.; WAALWIJK, C.; PAIVA, L. V.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. A greenhouse bioassay for the Fusarium oxysporum f. sp. cubense x ?Grand Naine? (Musa, AAA, Cavendish Subgroup) interaction. Acta Horticulturae, v. 897, p. 377-380, may 2011.Biblioteca(s): Embrapa Agroenergia. |
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10. | | DITA, M. A.; HERAI, R.; WAALWIJK, C.; YAMAGISHI, M.; GIACHETTO, P.; FERREIRA, G.; SOUZA, M.; KEMA, G. H. J. Comparative transcriptome analysis and genome assembly of Fusarium oxysporum f. sp. cubense. In: INTERNATIONAL PROMUSA SYMPOSIUM, 2011, Salvador. Bananas and plantains: toward sustainable global production and improved uses: abstracts. [S.l.]: ISHS, 2011. p. 58.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agricultura Digital. |
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13. | | DÍAZ-TRUJILLO, C.; KOBAYASHI, A. K.; ZWIERS, L. H; SOUZA JÚNIOR, M. T.; KEMA, G. H. J. Developing tools for Mycosphaerella fijiensis studies. Gewasbescherming, v. 40, n. 3, p. 129, mei 2009. Edição do Fast Firward 40 jaar Gewasbescherming, 2009, Wageningen.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio-Norte. |
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14. | | WAALWIJK, C.; DITA, M. A.; BUDDENHAGEN, I.; PAIVA, L. V. K.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Development of a detection method for tropical race 4 of Fusarium oxysporum f. sp. cubense. In: ISHS/PROMUSA BANANA SYMPOSIUM, 2009, Guangzhou, China. Global perspectives on asian challenges: programme and abstracts. Guangzhou: ISHS: Promusa, 2009. p.38.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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15. | | DÍAZ-TRUJILLO, C.; FORTES, C. F.; CAPDEVILLE, G. de; JALINK, H.; SOUZA, M.; KEMA, G. H. J. A reliable routine assay for black leaf streak disease of bananas caused by Mycosphaerella fijiensis. In: EUROPEAN CONFERENCE ON FUNGAL GENETICS, 9., 2008, Edinburgh. Meeting abstracts... Edinburgh: University of Edinburgh, 2008.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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16. | | MUNIZ, C. R.; SILVA, G. F. da; SOUZA JUNIOR, M. T.; FREIRE, F. das C. O.; KEMA, G. H. J.; GUEDES, M. I. F. Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants. Genetics and Molecular Research, v. 13, n. 2, p. 2906-2913, 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Agroindústria Tropical; Embrapa Amazônia Ocidental. |
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17. | | RODRIGUEZ, M. A. D.; WAALWIJK, C.; DIAZ, C.; D'HONT, A.; YAHIAOUE, N.; BAKRLY, F.; SOUZA JR. M.; KEMA, G. H. J. Characterization of Musa germplasm for resistance to tropical race 4 of fusarium oxysporum f. sp. cubense. In: PROMUSA SYMPOSIUM, 2011, Salvador. Bananas and plantains: toward sustainable global production and improved uses: abstracts. [Montepellier.]: [Bioversity International, 2011. p.37.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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18. | | RODRIGUEZ, M. A. D.; WAALWIJK, C.; DIAZ, C.; D´HON, A.; YAHIAOUI, N.; CARREEL, F.; BAKRY, F.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Characterization of Musa germplasm for resistance to tropical race 4 of Fusarium oxysporum f. sp. cubense. In: ISHS / PROMUSA SYMPOSIUM, 2011, Salvador, BH. Bananas and plantains: toward sustainable global production and improved uses: program and abstracts. Leuven: ISHS, 2011. p. 49Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agroenergia. |
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20. | | CAPDEVILLE, G.; SOUZA JÚNIOR, M. T.; SZINAY, D.; DINIZ, L.; WIJINKER, E.; DE JONG, J. H.; SWENNEN, R.; KEMA, G. H. J. High-resolution BAC-FISH in Musa ssp: Mapping, biotic stress related BAC clones. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 41., 2008, Belo Horizonte. [Resumos...]. Tropical Plant Pathology, v. 33, 2008. Suplemento. Resumo MEL-036. p. S208.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 42 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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