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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
19/01/2009 |
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Data da última atualização: |
24/07/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
KOBAYASHI, A. K.; DIAZ TRUJILLO, C.; LEE, T. van der; ZWIERS, L.-H.; PAIVA, L. V.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. |
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Afiliação: |
ADILSON KENJI KOBAYASHI, CPAMN; CAUCASELLA DIAZ TRUJILLO, PLANT RESEARCH INTERNATIONAL; THEO VAN DER LEE; LUTE-HARM ZWIERS; LUCIANO V. PAIVA, FEDERAL UNIVERSITY OF LAVRAS; MANOEL TEIXEIRA SOUZA JUNIOR, EMBRAPA LABEX EUROPE; GERT H. J. KEMA, PLANT RESEARCH INTERNATIONAL. |
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Título: |
Generation of transgenic Mycosphaerella fijiensis expressing reporter genes: tools for pathogenicity and mating studies. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: INTERNATIONAL MYCOSPHAERELLA AND STAGONOSPORA SYMPOSIUM, 7., 2008. Ascona, Switzerland. Program, Presentations, Abstracts. Ascona, Switzerland, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
Mycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducted in order to characterize the different stages in the pathogenesis on banana. MenosMycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducte... Mostrar Tudo |
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Palavras-Chave: |
CIRAD139A; CIRAD86; DsRed; GFP. |
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Thesagro: |
Doença; Fungo. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/69938/1/GenerationTransgenic-22676.pdf
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Marc: |
LEADER 02408nam a2200253 a 4500
001 1069938
005 2023-07-24
008 2008 bl uuuu u00u1 u #d
100 1 $aKOBAYASHI, A. K.
245 $aGeneration of transgenic Mycosphaerella fijiensis expressing reporter genes$btools for pathogenicity and mating studies.$h[electronic resource]
260 $aIn: INTERNATIONAL MYCOSPHAERELLA AND STAGONOSPORA SYMPOSIUM, 7., 2008. Ascona, Switzerland. Program, Presentations, Abstracts. Ascona, Switzerland$c2008
520 $aMycosphaerella fijiensis, the causal agent of black leaf streak disease is the most devastating pathogen of banana and plantains. Detailed studies on the plant pathogen interaction as well as mating behavior could be improved by the use of non-destructive reporter gene assays. M. fijiensis isolates from different global populations and opposite mating types, CIRAD86 (Mat1-1) from Cameroon and CIRAD139A (Mat1-2) from Colombia, were selected for our studies. In order to generate transgenic M. fijiensis strains, two gene constructs carrying either reporter genes gfp (green fluorescent protein) or dsred (Discosoma sp. red fluorescent protein) driven by the constitutive promoter PtoxA were used. Both constructs also carried the hph gene for resistance to the antibiotic hygromycin as selective marker. Transformation procedures mediated by Agrobacterium tumefaciens strain LBA1100 were carried out using macerated mycelium from 3-week-old cultures of CIRAD86 and CIRAD139A. After the cocultivation period, the cultures were transferred to selective medium containing 30 mg/l hygromycin. Transgenic events were identified by fluorescent microscopy observations after 2-3 weeks. In this part of the work, we were able to generate transgenic strains from both isolates expressing either reporter genes, namely CIRAD86::GFP, CIRAD86::DsRed, CIRAD139A::GFP and CIRAD139A::DsRed. Simultaneously, pathogenicity validation screens and mating assays using in vitro leaf fragments are also being conducted in order to characterize the different stages in the pathogenesis on banana.
650 $aDoença
650 $aFungo
653 $aCIRAD139A
653 $aCIRAD86
653 $aDsRed
653 $aGFP
700 1 $aDIAZ TRUJILLO, C.
700 1 $aLEE, T. van der
700 1 $aZWIERS, L.-H.
700 1 $aPAIVA, L. V.
700 1 $aSOUZA JUNIOR, M. T.
700 1 $aKEMA, G. H. J.
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Esconder MarcMostrar Marc Completo |
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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Biblioteca |
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Tipo/Formato |
Classificação |
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Registro |
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| Registros recuperados : 42 | |
| 22. |  | GARCIA, S. A. L.; TALEBI, R.; FERREIRA, C. F.; VROH, I.; PAIVA, L. V.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Identification and validation of EST-derived Molecular markers, TRAP and VNTR, for banana research. In: ISHS/PROMUSA BANANA SYMPOSIUM, 2009, Guangzhou, China. Global perspectives on asian challenges: programme and abstracts. Guangzhou: ISHS: Promusa, 2009. p. 10.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 23. | | GARCIA, S. A. L.; TALEBI, R.; FERREIRA, C. F.; VROH, B. I.; PAIVA, L. V.; KEMA, G. H. J.; SOUZA JUNIOR, M. T. Identification and validation of EST-Derived Molecular Markers, TRAP and VNTRs, for banana research Acta Horticulturae, v. 897, p. 69-80, may 2011.| Biblioteca(s): Embrapa Agroenergia. |
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| 24. | | KOBAYASHI, A. K.; DIAZ TRUJILLO, C.; LEE, T. van der; ZWIERS, L.-H.; PAIVA, L. V.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Generation of transgenic Mycosphaerella fijiensis expressing reporter genes: tools for pathogenicity and mating studies. In: INTERNATIONAL MYCOSPHAERELLA AND STAGONOSPORA SYMPOSIUM, 7., 2008. Ascona, Switzerland. Program, Presentations, Abstracts. Ascona, Switzerland, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
|  |
| 25. | | CAPDEVILLE, G.; SOUZA JÚNIOR, M. T.; SZINAY, D.; DINIZ, L.; WIJINKER, E.; DE JONG, J. H.; SWENNEN, R.; KEMA, G. H. J. High-resolution BAC-FISH in Musa ssp: Mapping, biotic stress related BAC clones. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 41., 2008, Belo Horizonte. [Resumos...]. Tropical Plant Pathology, v. 33, 2008. Suplemento. Resumo MEL-036. p. S208.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 27. | | RODRIGUEZ, M. A. D.; WAALWIJK, C.; MUTUA, P.; DALY, A.; CHANG, P. F. L.; CORCOLON, B. M.; KEMA, G. H. J. Detecting fusarium oxysporum f. Sp. cubense tropical race 4 in soil and symptomless banana tissues. In:PROMUSA SYMPOSIUM, 2011, Salvador. Bananas and plantains: toward sustainable global production and improved uses: abstracts. [Montepellier.]: [Bioversity International, 2011. p. 36.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 28. | | RODRIGUEZ, M. A. D.; WAALWIJK, C.; MUTUA, P.; DALY, A.; CHANG, P. F. L.; CORCOLON, B. M.; PAIVA, L.; SOUZA, M.; KEMA, G. H. J. Detecting Fusarium oxysporum f. sp. cubense tropical race 4 in soil and symptomless banana tissues. Acta Horticulturae, n. 986, p. 127-130, April, 2013. Trabalho apresentado no ISHS - ProMusa Symposium on Bananas and Plantains: Towards Sustainable Global Production and Improved Uses. Eds.: I. Van den Bergh et al.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 29. | | LOBO, I. K. C.; HANADA, R. E.; SOUSA, N. R.; GASPAROTTO, L.; SOUZA Jr. M. T.; KEMA, G. H. J.; SILVA, G. F. da. Construction of gateway binary vector for selection with bialaphos or carboxin and GFP expression in fungi. In: CONGRESSO BRASILEIRO DE GENÉTICA, 57., 2011, Águas de Lindóia. Resumos... s.l.: SBG, 2011. p. 25.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 30. | | CAPDEVILLE, G. D.; SOUZA JUNIOR, M. T.; SZINAY, D.; DINIZ, L. E. C.; WIJNKER, E.; SWENNEN, R.; KEMA, G. H. J.; DE JONG, H. The potential of high-resolution BAC-FISH in banana breeding. Euphytica, v.166, p. 431-443, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 31. | | CAPDEVILLE, G. de; SOUZA JUNIOR, M. T.; SZINAY, D.; DINIZ, L. E. C.; WIJNKER, E.; SWENNEN, R.; KEMA, G. H. J.; JONG, H. de. The potential of righ-resolution BAC-FISH in banana breeding. Euphytica, Wageningen, n. 166, p. 431-443, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 32. | | DÍAZ-TRUJILLO, C.; KOBAYASHI, A. K.; SOUZA JUNIOR, M. T.; CHONG, P.; MEIJER, H. J. G.; ISAZA, R. E. A.; KEMA, G. H. J. Targeted and random genetic modification of the black Sigatoka pathogen Pseudocercospora fijiensis by Agrobacterium tumefaciens-mediated transformation. Journal of Microbiological Methods, v. 148, p. 127-137, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 33. | | BERG, A. S.; DITA, M.; NAN, T.; SHEA, T.; ZHOU, S.; JONKERS, W.; ZHENG, Q.; YOUNG, S.; YAMAGISHI, M. E. B.; GIACHETTO, P. F.; HERAI, R.; SOUZA, M.; WAALWIJK, C.; KEMA, G. H. J.; KLISTLER, H.; MA, L. Genome sequencing of Fusarium oxysporum f. sp. cubense tropical race 4 strain II5. Phytopathology, v. 102, n. 7, 2012. Supplement 4. Abstracts of presentations of the APS Annual meeting, Providence, RI, 2012.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 34. | | FERREIRA, C. F.; Van der LEE, T. A. J.; QUIRÓS, O.; TE LINTEL HEKKERT, B.; ZAPATER, M. F.; GOODWIN, S. B.; GUZMÁN, M.; SOUZA JÚNIOR, M. T.; KEMA, G. H. J. Desenvolvimento de VNTRs para estudos de diversidade genética em Mycosphaerella fijiensis, causado por Sigatoka-negra em banananeiras (Musa spp.). In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 20.; ANNUAL MEETING OF THE INTERAMERICAN SOCIETY FOR TROPICAL HORTICULTURE, 54., 2008, Vitória. Frutas para todos: estratégias, tecnologias e visão sustentável: anais. Vitória: INCAPER: Sociedade Brasileira de Fruticultura, 2008.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 35. | | FERREIRA, C. F.; VAN DER LEE, T. A. J.; QUIROS, O.; TE LINTEL, H.; ZAPATER, M.; CARLIER, J.; GOODWIN, S.; GUZMAN, M.; SOUZA JÚNIOR, M.; KEMA, G. H. J. Development of VNTR markers to assess genetic diversity of Mycosphaerella fijiensis, the causal agent of black leaf streak disease in bananas (Musa spp.). UNITED STATES DEPARTMENT OF AGRICULTURAL. Agricultural Research Service. Crop production and pest control research. Disponível em: . Acesso em: 25 fev. 2010| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 36. | | FERREIRA, C.; Van der LEE, T. A. J.; QUIROS, O.; HERRERT, B. L.; CARLIER, J.; TRUJILLO, C. D.; GOODWIN, S. B.; GUZMAN, M.; SOUZA, M.; KEMA, G. H. J. Development of cost effective tools for assessing genetic diversity of Mycosphaerella fijiensis, the causal agent of black-Sigatoka disease in bananas (Musa spp.). In: EUROPEAN CONFERENCE ON FUNGAL GENETICS, 9., 2008, Edinburgh. Meeting abstracts... Edinburgh: University of Edinburgh, 2008. PR9.29| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 37. | | MUNIZ, C. R.; FREIRE, F. das C. O.; VIANA, F. M. P.; CARDOSO, J. E.; CORREIA, D.; JALINK, H.; KEMA, G. H. J.; SILVA, G. F. da; GUEDES, M. I. F. Polyclonal antibody-based ELISA in combination with specific PCR amplification of internal transcribed spacer regions for the detection and quantitation of Lasiodiplodia theobromae, causal agent of gummosis in cashew nut plants. Annals of Applied Biology, v. 160, n. 3, p. 217-224, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 38. | | MUNIZ, C. R.; FREIRE, F. das C. O.; VIANA, F. M. P.; CARDOSO, J. E.; CORREIA, D.; JALINK, H.; KEMA, G. H. J.; SILVA, G. F. da; GUEDES, M. I. F. Polyclonal antibody-based ELISA in combination with specific PCR amplification of internal transcribed spacer regions for the detection and quantitation of Lasioplodia theobromae, causal agent of gummosis in cashew nut plants. Annals of Applied Biology, v.160, p. 217-224, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 39. | | GARCIA, S. A. I.; VAN DER LEE, T. A. J.; FERREIRA, C. F.; HEKKERT, B. T. L.; ZAPATER, M.-F.; GOODWIN, S. B.; GUZMÁN, M.; KEMA, G. H. J.; SOUZA JUNIOR, M. T. Variable number of tandem repeat markers in the genome sequence of Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana (Musa spp). Genetics and Molecular Research, v. 9, n. 4, p. 2207-2212, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 40. | | ISAZA, R. E. A.; DIAZ-TRUJILLO, C.; DHILLON, B.; AERTS, A.; CARLIER, J.; CRANE, C. F.; JONG, T. V.; VRIES, I.; DIETRICH, R.; FARMER, A. D.; FEREIRA, C. F.; GARCIA, S.; GUZMAN, M.; HAMELIN, R. C.; LINDQUIST, E. A.; MEHRABI, R.; QUIROS, O.; SCHMUTZ, J.; SHAPIRO, H.; REYNOLDS, E.; SCALLIET, G.; SOUZA JUNIOR, M. T.; STERGIOPOULOS, I.; VAN DER LEE, T. A. J.; WIT, P. J. G. M. DE; ZAPATER, M.-F.; ZWIERS, L.-H.; GRIGORIEV, I. V.; GOODWIN, S. B.; KEMA, G. H. J. Combating a global threat to a clonal crop: banana black sigatoka pathogen Pseudocercospora fijiensis (Synonym Mycosphaerella fijiensis) genomes reveal clues for disease control. PLOS Genetics, Aug. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 42 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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