Data/hora: 10/06/2024 03:32:08

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	25/02/2010	
Data da última atualização: 	29/05/2023	
Autoria: 	FERREIRA, C. F.; VAN DER LEE, T. A. J.; QUIROS, O.; TE LINTEL, H.; ZAPATER, M.; CARLIER, J.; GOODWIN, S.; GUZMAN, M.; SOUZA JÚNIOR, M.; KEMA, G. H. J.	
Afiliação: 	CLAUDIA FORTES FERREIRA, CNPMF; Theodoor Angelo Jozef Van Der Lee, PRIN; Olman Quirós, CORBANA; Bas Te Lintel Hekkert, PRIN; Marie Francoise Zapater, CIRAD; Jean Carlier, CIRAD; Stephen B. Goodwin; Mauricio Guzman, CORBANA; Manoel Teixeira Souza Júnior, PRIN; Gert H. J. Kema, PRIN.	
Título: 	Development of VNTR markers to assess genetic diversity of Mycosphaerella fijiensis, the causal agent of black leaf streak disease in bananas (Musa spp.).	
Ano de publicação: 	2008	
Fonte/Imprenta: 	UNITED STATES DEPARTMENT OF AGRICULTURAL. Agricultural Research Service. Crop production and pest control research.	
Idioma: 	Inglês	
Notas: 	Disponível em: <http://www.ars.usda.gov/research/publications/publications.htm?seq_no_115=231337 >. Acesso em: 25 fev. 2010	
Conteúdo: 	Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana, commonly known as Black Sigatoka, is one of the most devastating plant pathogens. Black leaf streak was first discovered in Fiji during the 1960s and the pathogen still actively colonizes new areas where bananas are grown. Using the genome sequence of M. fijiensis isolate CIRAD86 (http://genome.jgi-psf.org/Mycfi1/Mycfi1.home.html), we identified Variable Number of Tandem Repeat (VNTR) markers using a bioinformatics pipeline. The VNTR screening resulted in 1528 candidate VNTR loci of which many were imperfect mono-, di-, or tri-nucleotide repeats. Sixteen VNTR markers were selected based on repeat type, repeat length and the number of units of the repeat. Primers for these 16 VNTR markers were tested on a set of M. fijiensis isolates and five VNTR markers that showed multiple alleles were tested on M. fijiensis field isolates and on progeny of the cross between isolates CIRAD86 and CIRAD 139a. Numbers of alleles identified varied from 2 to 5 per locus. The markers allowed high throughput and robust scoring on agarose gels and proved useful for genetic diversity and population genetic studies using basic molecular instrumentation. We envisage that this set will enable more detailed analyses of the large-scale migration pattern of this important pathogen as well as small-scale effects of fungicide treatments on the population diversity in banana plantations.	
Palavras-Chave: 	Melhoramento genético.	
Thesagro: 	Doença de Planta; Fungo.	
Categoria do assunto: 	G Melhoramento Genético	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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