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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
01/10/2009 |
Data da última atualização: |
04/02/2010 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
DITA, M. A.; WAALWIJK, C.; PAIVA, L. V.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. |
Afiliação: |
Miguel Angel Dita Rodríguez, CNPMF; C. Waalwijk, PRI-Plant Research International; Luciano Vilela Paiva, UFLA; Manoel Teixeira Souza Junior, Embrapa LABEX Europe; Gert H. J. Kema, PRI-Plant Research International. |
Título: |
A greenhouse bio-assay for the Fusarium oxysporum f. sp. cubense (Tropical Race 4) X banana (Cavendish subgroup) interaction. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
In: ISHS/PROMUSA BANANA SYMPOSIUM, 2009, Guangzhou, China. Global perspectives on asian challenges: programme and abstracts. Guangzhou: ISHS: Promusa, 2009. p. 45. |
Idioma: |
Inglês |
Conteúdo: |
Several disease resistance screening protocols for fusarium wilt of banana (causal agent Fusarium oxysporum f. sp. cubense-foc) under greenhouse conditions have been reported. However, a standard and worldwide accepted methodology is still undefined. A standardised greenhouse bioassay for this pathosystem is necessary for the identification of resistant banana genotypes and for detailed plant-pathogen interaction studies. This became especially important with the advent of tropical race 4 ( TR4), which is a significant threat for the global Cavendish-based banana export industry. Thiswork aimed to establish a reliable and rapid greenhouse biossayfor foc with a focus on the TR4- cavendish interaction. Using a doublepot system, hardened 3-months-old tissue-culture plants of 'Grand Naine' (AAA, Cavendish subgroup) were, separately, inoculated with three isolates, belonging to the Vegetative Compatiblility Group (VCG) 01213 and considered as TR4, and one isolate, ranked as race 1 with known pathogenicity on 'Silk' (AAB). Plants were inoculated by root dipping (30 minutes, 105 conidia/mL) and then transferred to pots partially filled with sand supplemented with 20 maize kernels, previously colonised with foc for 10 days. In TR4-inoculated plants, first symptoms appeared at 7 days after inoculation (dai) showing the typical external (yellowing) and internal (rhizome discoloration) symptoms at 14 dai. At 40 dai, TR4-inoculated plants showed severe wilting and internal necrosis even in the pseudostem. No symptoms were observed in plants inoculate with the race 1 isolate. All the TR4 isolates caused similar symptoms, and no differences regarding incubation period or severity were observed. In addition, all TR4 isolates were successfully recovered from the symptomatic rhizomes on Komada's medium. These results suggest that the bio-assay here described is suitable to screen banana germplasm for TR4 resistance, to discriminate Foc races and to help studies to elucidate the defence mechanism underlyng the Foc-banana interaction. MenosSeveral disease resistance screening protocols for fusarium wilt of banana (causal agent Fusarium oxysporum f. sp. cubense-foc) under greenhouse conditions have been reported. However, a standard and worldwide accepted methodology is still undefined. A standardised greenhouse bioassay for this pathosystem is necessary for the identification of resistant banana genotypes and for detailed plant-pathogen interaction studies. This became especially important with the advent of tropical race 4 ( TR4), which is a significant threat for the global Cavendish-based banana export industry. Thiswork aimed to establish a reliable and rapid greenhouse biossayfor foc with a focus on the TR4- cavendish interaction. Using a doublepot system, hardened 3-months-old tissue-culture plants of 'Grand Naine' (AAA, Cavendish subgroup) were, separately, inoculated with three isolates, belonging to the Vegetative Compatiblility Group (VCG) 01213 and considered as TR4, and one isolate, ranked as race 1 with known pathogenicity on 'Silk' (AAB). Plants were inoculated by root dipping (30 minutes, 105 conidia/mL) and then transferred to pots partially filled with sand supplemented with 20 maize kernels, previously colonised with foc for 10 days. In TR4-inoculated plants, first symptoms appeared at 7 days after inoculation (dai) showing the typical external (yellowing) and internal (rhizome discoloration) symptoms at 14 dai. At 40 dai, TR4-inoculated plants showed severe wilting and internal necrosis eve... Mostrar Tudo |
Thesagro: |
Banana; Doença de Planta; Fusariose. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02770naa a2200205 a 4500 001 1655762 005 2010-02-04 008 2009 bl uuuu u00u1 u #d 100 1 $aDITA, M. A. 245 $aA greenhouse bio-assay for the Fusarium oxysporum f. sp. cubense (Tropical Race 4) X banana (Cavendish subgroup) interaction. 260 $c2009 520 $aSeveral disease resistance screening protocols for fusarium wilt of banana (causal agent Fusarium oxysporum f. sp. cubense-foc) under greenhouse conditions have been reported. However, a standard and worldwide accepted methodology is still undefined. A standardised greenhouse bioassay for this pathosystem is necessary for the identification of resistant banana genotypes and for detailed plant-pathogen interaction studies. This became especially important with the advent of tropical race 4 ( TR4), which is a significant threat for the global Cavendish-based banana export industry. Thiswork aimed to establish a reliable and rapid greenhouse biossayfor foc with a focus on the TR4- cavendish interaction. Using a doublepot system, hardened 3-months-old tissue-culture plants of 'Grand Naine' (AAA, Cavendish subgroup) were, separately, inoculated with three isolates, belonging to the Vegetative Compatiblility Group (VCG) 01213 and considered as TR4, and one isolate, ranked as race 1 with known pathogenicity on 'Silk' (AAB). Plants were inoculated by root dipping (30 minutes, 105 conidia/mL) and then transferred to pots partially filled with sand supplemented with 20 maize kernels, previously colonised with foc for 10 days. In TR4-inoculated plants, first symptoms appeared at 7 days after inoculation (dai) showing the typical external (yellowing) and internal (rhizome discoloration) symptoms at 14 dai. At 40 dai, TR4-inoculated plants showed severe wilting and internal necrosis even in the pseudostem. No symptoms were observed in plants inoculate with the race 1 isolate. All the TR4 isolates caused similar symptoms, and no differences regarding incubation period or severity were observed. In addition, all TR4 isolates were successfully recovered from the symptomatic rhizomes on Komada's medium. These results suggest that the bio-assay here described is suitable to screen banana germplasm for TR4 resistance, to discriminate Foc races and to help studies to elucidate the defence mechanism underlyng the Foc-banana interaction. 650 $aBanana 650 $aDoença de Planta 650 $aFusariose 700 1 $aWAALWIJK, C. 700 1 $aPAIVA, L. V. 700 1 $aSOUZA JUNIOR, M. T. 700 1 $aKEMA, G. H. J. 773 $tIn: ISHS/PROMUSA BANANA SYMPOSIUM, 2009, Guangzhou, China. Global perspectives on asian challenges: programme and abstracts. Guangzhou: ISHS: Promusa, 2009. p. 45.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registros recuperados : 52 | |
2. | | DITA, M.; HERAI, R.; YAMAGISHI, M.; FERREIRA, G.; SOUZA, M.; GIACHETTO, P.; WAALWIJK, C.; KEMA, G. Comparative transcriptome analyses of Fusarium oysporum f. sp. cubense. Phytopathology, v. 101, n. 6, Supplement, p. S43, 2011. Trabalho apresentado no: APS-IPPC 2011 Joint Meeting in Honolulu, Hawaii, August 6-10, 2011.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agricultura Digital. |
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3. | | DITA, M. A.; WAALWIJK, C.; PAIVA, L.; KEMA, G.; SOUZA JUNIOR, M. Kit diagnóstico para a maior ameaça bananicultura mundial. In: SIMPÓSIO SOBRE INOVAÇÃO E CRIATIVIDADE CIENTÍFICA NA EMBRAPA, 2., 2010, Brasília, DF. Anais... Brasília, DF: Embrapa, 2010. Disponível em: Acesso em: 21 fev. 2011.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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4. | | DITA, M. A.; WAALWIJK, C.; PAIVA, L.; KEMA, G.; SOUZA JUNIOR, M. T. Kit diagnóstico para a raça 4 tropical de Fusarium oxysporum f. sp. cubense, agente causal do mal-do-Panamá da bananeira. In: SIMPÓSIO SOBRE INOVAÇÃO E CRIATIVIDADE CIENTÍFICA NA EMBRAPA, 2., 2010, Brasília, DF. Anais... Brasília, DF: Embrapa, 2010. Disponível em: Acesso em: 21 fev. 2011.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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8. | | DITA, M. A.; KOBAYASHI, A. K.; WAALWIJK, C.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Genetic transformation of Fusarium oxysporum f. sp. cubense. Tropical Plant Pathology, v. 34, p. S177, ago. 2009. Suplemento. Ref. 606. Edição dos Resumos do 42° Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio-Norte. |
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9. | | KOBAYASHI, A. K.; RODRIGUEZ, M. A. D.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Generation of Fusarium f. sp. passiflorae strains expressing reporter genes. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 607.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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11. | | HERAI, R.; DITA, M.; WAALWIJK, C.; FERREIRA, G.; SOUZA, M.; KEMA, G.; FALCAO, P.; GIACHETTO, P.; YAMAGISHI, M. Building a transcriptome molecular marker platform for diagnosis of fungal plant pathogens. In: INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY, 8., 2012, Campinas. Abstract book... Ribeirão Preto: AB3C, 2012. Não paginado. X-MEETING 2012.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agricultura Digital. |
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14. | | DÍAZ-TRUJILLO, C.; KOBAYASHI, A. K.; ZWIERS, L. H; SOUZA JÚNIOR, M. T.; KEMA, G. H. J. Developing tools for Mycosphaerella fijiensis studies. Gewasbescherming, v. 40, n. 3, p. 129, mei 2009. Edição do Fast Firward 40 jaar Gewasbescherming, 2009, Wageningen.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio-Norte. |
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15. | | WAALWIJK, C.; DITA, M. A.; BUDDENHAGEN, I.; PAIVA, L. V. K.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Development of a detection method for tropical race 4 of Fusarium oxysporum f. sp. cubense. In: ISHS/PROMUSA BANANA SYMPOSIUM, 2009, Guangzhou, China. Global perspectives on asian challenges: programme and abstracts. Guangzhou: ISHS: Promusa, 2009. p.38.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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16. | | DITA, M. A.; HERAI, R.; WAALWIJK, C.; YAMAGISHI, M.; GIACHETTO, P.; FERREIRA, G.; SOUZA, M.; KEMA, G. H. J. Comparative transcriptome analysis and genome assembly of Fusarium oxysporum f. sp. cubense. In: INTERNATIONAL PROMUSA SYMPOSIUM, 2011, Salvador. Bananas and plantains: toward sustainable global production and improved uses: abstracts. [S.l.]: ISHS, 2011. p. 58.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agricultura Digital. |
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17. | | KOBAYASHI, A. K.; DITA, M. A.; TROCOLI, R. O.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. GFP and RFP transformation of Fusarium guttiforme (sin. Fusarium subglutinans f. sp. annanas). Tropical Plant Pathology, v. 34, p. S156, ago. 2009. Suplemento. Ref. 545. Edição dos Resumos do 42° Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Meio-Norte. |
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18. | | RODRIGUEZ, M. A. D.; WAALWIJK, C.; PAIVA, L. V.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. A greenhouse bioassay for the Fusarium oxysporum f. sp. cubense x ?Grand Naine? (Musa, AAA, Cavendish Subgroup) interaction. Acta Horticulturae, v. 897, p. 377-380, may 2011.Biblioteca(s): Embrapa Agroenergia. |
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19. | | RODRIGUEZ, M. A. D.; KOBAYASHI, A. K.; WAALWIJK, C.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Genetic transformation of Fusarium oxysporum f. sp. cubense. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 606.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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20. | | DÍAZ-TRUJILLO, C.; FORTES, C. F.; CAPDEVILLE, G. de; JALINK, H.; SOUZA, M.; KEMA, G. H. J. A reliable routine assay for black leaf streak disease of bananas caused by Mycosphaerella fijiensis. In: EUROPEAN CONFERENCE ON FUNGAL GENETICS, 9., 2008, Edinburgh. Meeting abstracts... Edinburgh: University of Edinburgh, 2008.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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Registros recuperados : 52 | |
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