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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
27/03/2013 |
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Data da última atualização: |
04/07/2016 |
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Autoria: |
ALVES, C. J.; FIGUEIREDO, S. M. de; AZEVEDO, S. S. de; CLEMENTINO, I. J.; KEID, I. B.; VASCONCELLOS, S. A.; BATISTA, C. de S. A.; ROCHA, V. C. M.; HIGINO, S. S. |
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Título: |
Detection of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 41, n. 2, p. 365-367, 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
To determine the presence of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil, 80 animals slaughtered in the public slaughterhouse of Patos city were used. Before slaughter, blood samples were collected by jugular venopuncture from each animal, and after slaughter, testicles, epidydimus and uterus were aseptically collected. For the serological diagnosis of B. ovis and B. abortus infections, the agar gel immunodiffusion (AGID) and Rose Bengal (RBT) tests were carried out, respectively. In addition, microbiological culture and polymerase chain reaction (PCR) were performed on testicle, epidydimus and uterus samples. Six animals (7.5%) tested positive for the presence of B. ovis antibodies and all animals tested negative for the presence of B. abortus antibodies. One AGID-positive animal tested positive at uterine swab culture. PCR was able to amplify DNA of Brucella spp. from the pool of testicle, epidydimus and uterus samples from AGID-positive animals. This is the first report of isolation and detection of B. ovis DNA by PCR in ovine from the Northeast region of Brazil. |
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Palavras-Chave: |
Brucella ovis; PCR. |
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Thesagro: |
Caprino; Doença animal; Isolamento. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01918naa a2200277 a 4500
001 1954316
005 2016-07-04
008 2010 bl uuuu u00u1 u #d
100 1 $aALVES, C. J.
245 $aDetection of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil.$h[electronic resource]
260 $c2010
520 $aTo determine the presence of Brucella ovis in ovine from Paraíba State, in the Northeast region of Brazil, 80 animals slaughtered in the public slaughterhouse of Patos city were used. Before slaughter, blood samples were collected by jugular venopuncture from each animal, and after slaughter, testicles, epidydimus and uterus were aseptically collected. For the serological diagnosis of B. ovis and B. abortus infections, the agar gel immunodiffusion (AGID) and Rose Bengal (RBT) tests were carried out, respectively. In addition, microbiological culture and polymerase chain reaction (PCR) were performed on testicle, epidydimus and uterus samples. Six animals (7.5%) tested positive for the presence of B. ovis antibodies and all animals tested negative for the presence of B. abortus antibodies. One AGID-positive animal tested positive at uterine swab culture. PCR was able to amplify DNA of Brucella spp. from the pool of testicle, epidydimus and uterus samples from AGID-positive animals. This is the first report of isolation and detection of B. ovis DNA by PCR in ovine from the Northeast region of Brazil.
650 $aCaprino
650 $aDoença animal
650 $aIsolamento
653 $aBrucella ovis
653 $aPCR
700 1 $aFIGUEIREDO, S. M. de
700 1 $aAZEVEDO, S. S. de
700 1 $aCLEMENTINO, I. J.
700 1 $aKEID, I. B.
700 1 $aVASCONCELLOS, S. A.
700 1 $aBATISTA, C. de S. A.
700 1 $aROCHA, V. C. M.
700 1 $aHIGINO, S. S.
773 $tBrazilian Journal of Microbiology$gv. 41, n. 2, p. 365-367, 2010.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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