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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
13/11/2015 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, F. V. D.; KAYA, E.; VIEIRA, L. de J.; SOUZA, E. H. de; AMORIM, V. B. de O.; SKOGERBOE, D.; MATSUMOTO, T.; ALVES, A. A. C.; LEDO, C. A. da S.; JENDEREK, M. M. |
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Afiliação: |
FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; ERGUN KAYA, National Center for Genetic Resources Preservation; LÍVIA DE JESUS VIEIRA; EVERTON HILO DE SOUZA; VANUSIA BATISTA DE OLIVEIRA AMORIM; DIANNE SKOGERBOE, National Center for Genetic Resources Preservation; TRACIE MATSUMOTO, Pacific Basin Agriculture Research Center; ALFREDO AUGUSTO CUNHA ALVES, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF; MARIA M. JENDEREK, National Center for Genetic Resources Preservation. |
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Título: |
Droplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v.123, n.2, nov. 2015. |
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ISSN: |
0167-6857 |
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DOI: |
10.1007/s11240-015-0899-8 |
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Idioma: |
Português |
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Conteúdo: |
Germplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus? genetic diversity, to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation, that is done by storing cells or tissues at ultra-low temperature in liquid nitrogen (-196 C). Droplet-vitrification, a combination of droplet freezing and solution-based vitrification, was used to establish a protocol for cryopreservation of pineapple genetic resources. This protocol was tested on cultivated and wild pineapple genotypes to establish a long-term germplasm security duplicate as well as to investigate cryo-injuries in the tissues by means of histological techniques. Excised shoot tips (0.5?1 mm with one primordial leaf) of different pineapple genotypes were precultured for 48 h on solid MS medium containing 0.3 M of sucrose. Three PVS2 exposure times (30, 45 and 60 min) were tested. The results showed high post cryopreservation survival for all genotypes evaluated. The best PVS2 exposure time varied according to genotype, although 45 min gave the best survival for the majority of genotypes. The technique was highly efficient in cryopreserving meristem shoot tips of different pineapple genotypes, and was also less laborious than techniques previously reported. This is a first report on application of the droplet-vitrification technique to diverse genotypes of cultivated and wild pineapples and the first report on histological changes occurring in cryopreserved Ananas tissue. MenosGermplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus? genetic diversity, to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation, that is done by storing cells or tissues at ultra-low temperature in liquid nitrogen (-196 C). Droplet-vitrification, a combination of droplet freezing and solution-based vitrification, was used to establish a protocol for cryopreservation of pineapple genetic resources. This protocol was tested on cultivated and wild pineapple genotypes to establish a long-term germplasm security duplicate as well as to investigate cryo-injuries in the tissues by means of histological techniques. Excised shoot tips (0.5?1 mm with one primordial leaf) of different pineapple genotypes were precultured for 48 h on solid MS medium containing 0.3 M of sucrose. Three PVS2 exposure times (30, 45 and 60 min) were tested. The results showed high post cryopreservation survival for all genotypes evaluated. The best PVS2 exposure time varied according to genotype, although 45 min gave the best survival for the majority of genotypes. The technique was highly efficient in cryopreserving meristem shoot tips of different pineapple genotypes, and was also less laborious than techniques previously reported. This is a first report on application of the droplet-vitrification technique to diverse genotypes of cultivated and wild pineapples and the ... Mostrar Tudo |
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Thesagro: |
Abacaxi; Ananás Comosus. |
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Thesaurus Nal: |
Histology; Pineapples. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02476naa a2200301 a 4500
001 2028518
005 2023-05-18
008 2015 bl uuuu u00u1 u #d
022 $a0167-6857
024 7 $a10.1007/s11240-015-0899-8$2DOI
100 1 $aSOUZA, F. V. D.
245 $aDroplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes.$h[electronic resource]
260 $c2015
520 $aGermplasm conservation of pineapple [Ananas comosus (L.) Mer.] is crucial to preserve the genus? genetic diversity, to secure material for genetic improvement and to support innovative and new research. Long-term conservation is accomplished through cryopreservation, that is done by storing cells or tissues at ultra-low temperature in liquid nitrogen (-196 C). Droplet-vitrification, a combination of droplet freezing and solution-based vitrification, was used to establish a protocol for cryopreservation of pineapple genetic resources. This protocol was tested on cultivated and wild pineapple genotypes to establish a long-term germplasm security duplicate as well as to investigate cryo-injuries in the tissues by means of histological techniques. Excised shoot tips (0.5?1 mm with one primordial leaf) of different pineapple genotypes were precultured for 48 h on solid MS medium containing 0.3 M of sucrose. Three PVS2 exposure times (30, 45 and 60 min) were tested. The results showed high post cryopreservation survival for all genotypes evaluated. The best PVS2 exposure time varied according to genotype, although 45 min gave the best survival for the majority of genotypes. The technique was highly efficient in cryopreserving meristem shoot tips of different pineapple genotypes, and was also less laborious than techniques previously reported. This is a first report on application of the droplet-vitrification technique to diverse genotypes of cultivated and wild pineapples and the first report on histological changes occurring in cryopreserved Ananas tissue.
650 $aHistology
650 $aPineapples
650 $aAbacaxi
650 $aAnanás Comosus
700 1 $aKAYA, E.
700 1 $aVIEIRA, L. de J.
700 1 $aSOUZA, E. H. de
700 1 $aAMORIM, V. B. de O.
700 1 $aSKOGERBOE, D.
700 1 $aMATSUMOTO, T.
700 1 $aALVES, A. A. C.
700 1 $aLEDO, C. A. da S.
700 1 $aJENDEREK, M. M.
773 $tPlant Cell, Tissue and Organ Culture$gv.123, n.2, nov. 2015.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 8 | |
| 4. |  | KAYA, E.; ALVES, A. A. C.; RODRIGUES, L.; JENDEREK, M.; HERNANDEZ-ELLIS, M.; OZUDOGRU, A.; ELLIS, D. Cryopreservation of eucalyptus genetic resources. Cryo-Letters, v. 34, n.6, p.608-618, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 5. | | SOUZA, F. V. D.; SOUZA, E. H. de; KAYA, E.; VIEIRA, L. de J.; SILVA, R. L. da. Cryopreservation of pineapple Shoot Tips by the droplet vitrification technique. In: LOYOLA-VARGAS, V.r M; Ochoa-Alejo, N. (ed.). Plant Cell Culture Protocols, Methods in Molecular Biology. New York, NY: Humana Press, 2018. 269-277 v.1815. Cap. 18.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 7. | | SOUZA, F. V. D.; KAYA, E.; VIEIRA, L. de J.; SOUZA, E. H. de; AMORIM, V. B. de O.; SKOGERBOE, D.; MATSUMOTO, T.; ALVES, A. A. C.; LEDO, C. A. da S.; JENDEREK, M. M. Droplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes. Plant Cell, Tissue and Organ Culture, v.123, n.2, nov. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 8. | | SOUZA, F. V. D.; KAYA, E.; VIEIRA, L. de J.; SOUZA, A. da S.; CARVALHO, M. de J. da S.; SANTOS, E. B.; ALVES, A. A. C.; ELLIS, D. Cryopreservation of Hamilin sweet orange [(Citrus sinensis (L.) Osbeck)] embryogenic calli using a modified aluminum cryo-plate technique. Scientia Horticulturae, v.224, p.302-305, October ,2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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