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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Rondônia. |
Data corrente: |
05/06/2020 |
Data da última atualização: |
15/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
RODRIGUES, S. A. D.; PONTELO, T. P.; KUSSANO, N. R.; KAWAMOTO, T. S.; LEME, L. O.; CAIXETA, F. M. C.; PFEIFER, L. F. M.; FRANCO, M. M.; DODE, M. A. N. |
Afiliação: |
SARAH A. D. RODRIGUES, Universidade de Brasília; THAIS P. PONTELO, Universidade Federal de Lavras; NAYARA R. KUSSANO, Universidade de Brasília; TAYNAN S. KAWAMOTO, Universidade Federal de Uberlândia; LIGIANE O. LEME, Universidade Federal do Espírito Santo; FELIPPE M. C. CAIXETA, Universidade de Brasília; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO; MAURICIO MACHAIM FRANCO, Cenargen; MARGOT ALVES NUNES DODE, Cenargen. |
Título: |
Effects of Prostaglandins E2 and F2alpha on the in vitro maturation of bovine oocytes. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Domestic Animal Endocrinology, v. 72, article 106447, 2020. |
ISSN: |
0739-7240 |
DOI: |
https://doi.org/10.1016/j.domaniend.2020.106447 |
Idioma: |
Inglês |
Conteúdo: |
We aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in vitro embryo production or embryo quality (P > 0.05). During maturation, the relative abundance of PTGS2 transcripts increased (P < 0.05) only in the less-competent group, whereas those of PTGES1 increased in the less-competent and in the more-competent groups. Conversely, AKR1B1 expression decreased only in the less-competent group (P < 0.05). Receptors for the PGE2 and PGF2a genes were very low or undetectable in oocytes. In conclusion, PGE2 and PGF2a are not recommended for media supplementation during maturation because they have no effect on embryo development. Although genes related to PGs activity are differentially expressed in cumulus cells of cumulus-oocyte complexes of different competence during maturation, the expression of PGE2 and PGF2a receptor genes was either not detectable or was detected at low levels in oocytes. MenosWe aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in vitro embryo production or embryo quality (P > 0.05). During maturation, the relative abundance of PTGS2 transcripts increased (P < 0.05) only in the less-competent group, whereas those of PTGES1 increased in the less-competent and in the more-competent groups. Conversely, AKR1B1 expression decreased only in the less-competent group (P < 0.05). Receptors for the PGE2 and PGF2a genes were very low or undetectable in oocytes. In conclusion, PGE2 and PGF2a are not recommended for media supplementation during maturation because they have no effect on embryo development. Although genes related to PGs activity are differentially expressed in cumulus cells of cumulus-oocyte complexes of different competence during maturation, the... Mostrar Tudo |
Palavras-Chave: |
E2; Embryonic development; F2a; Maturation; Oócito; Oocyte competence. |
Thesagro: |
Bovinocultura; Cultura In Vitro; Embriogénese; Maturação; Ovulo; Prostaglandina; Reprodução Animal. |
Thesaurus Nal: |
Animal reproduction; Cattle; Embryogenesis; Gene expression; In vitro studies; Oocytes; Prostaglandins. |
Categoria do assunto: |
-- K Ciência Florestal e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219006/1/cpafro-18481.pdf
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Marc: |
LEADER 02942naa a2200481 a 4500 001 2127907 005 2021-09-15 008 2020 bl uuuu u00u1 u #d 022 $a0739-7240 024 7 $ahttps://doi.org/10.1016/j.domaniend.2020.106447$2DOI 100 1 $aRODRIGUES, S. A. D. 245 $aEffects of Prostaglandins E2 and F2alpha on the in vitro maturation of bovine oocytes.$h[electronic resource] 260 $c2020 520 $aWe aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in vitro embryo production or embryo quality (P > 0.05). During maturation, the relative abundance of PTGS2 transcripts increased (P < 0.05) only in the less-competent group, whereas those of PTGES1 increased in the less-competent and in the more-competent groups. Conversely, AKR1B1 expression decreased only in the less-competent group (P < 0.05). Receptors for the PGE2 and PGF2a genes were very low or undetectable in oocytes. In conclusion, PGE2 and PGF2a are not recommended for media supplementation during maturation because they have no effect on embryo development. Although genes related to PGs activity are differentially expressed in cumulus cells of cumulus-oocyte complexes of different competence during maturation, the expression of PGE2 and PGF2a receptor genes was either not detectable or was detected at low levels in oocytes. 650 $aAnimal reproduction 650 $aCattle 650 $aEmbryogenesis 650 $aGene expression 650 $aIn vitro studies 650 $aOocytes 650 $aProstaglandins 650 $aBovinocultura 650 $aCultura In Vitro 650 $aEmbriogénese 650 $aMaturação 650 $aOvulo 650 $aProstaglandina 650 $aReprodução Animal 653 $aE2 653 $aEmbryonic development 653 $aF2a 653 $aMaturation 653 $aOócito 653 $aOocyte competence 700 1 $aPONTELO, T. P. 700 1 $aKUSSANO, N. R. 700 1 $aKAWAMOTO, T. S. 700 1 $aLEME, L. O. 700 1 $aCAIXETA, F. M. C. 700 1 $aPFEIFER, L. F. M. 700 1 $aFRANCO, M. M. 700 1 $aDODE, M. A. N. 773 $tDomestic Animal Endocrinology$gv. 72, article 106447, 2020.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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Registros recuperados : 20 | |
2. | | KAWAMOTO, T. S.; VIANA, J. H. M.; FARIA, O. A. C.; FIGUEIREDO, R. A. Ovarian characteristics and somatic development in 2 to 5 months old female Nelore (Bos taurus indicus) calves. Animal Reproduction, v. 15, Suppl.1, p.1086, 2018. Edição dos resumos do 10th International Ruminant Reproduction Symposium (IRRS 2018), 2018., Foz do Iguaçu-PR. CBRA.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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4. | | RODRIGUES, S. A. D.; PONTELO, T. P.; KAWAMOTO, T. S.; LEME, L. O.; FERNANDES, G. O.; DODE, M. A. N. Characterization of expression pattern of genes involved on the activity of PGE2 and PGF2alfa in bovine COCs with different levels of competence during in vitro maturation. Animal Reproduction, v. 15, n. 3, p. 429, Jul./Sept. 2018. Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018. Abstracts.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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5. | | FIDELIS, A. A. G.; MELO, F. R.; LEME, L. O.; KAWAMOTO, T. S.; DODE, M. A. N. Ethanolic extracts of cerrado plants on the oxidative stress of in vitro-produced bovine embryos. Animal Reproduction, v. 15, n. 3, p. 408, Jul./Sept. 2018. Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018. Abstracts.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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7. | | RODRIGUES, S. de A. D.; PONTELO, T. P.; KAWAMOTO, T. S.; CAIXETA, F. M. C.; DODE, M. A. N. Study on nuclear maturation kinetics of bovine ovocyts with different degrees of competence. Animal Reprodroduction, v. 14, n. 3, p. 798, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. AbstractsTipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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8. | | KAWAMOTO, T. S.; ZACARIAS, T. A.; GUIMARÃES, A. L. S.; SCALIANTE, J. R.; RODRIGUES, S. de A. D.; FIGUEIREDO, R. A. Serum FSH, AMH related to number and morphology of oocytes in superovulated 4 to 7 months old Nelore breed females (Bos Taurus Indicus). Animal Reproduction, v.14, n.3, p.757, jul./set. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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9. | | KAWAMOTO, T. S.; VIANA, J. H. M.; PONTELO, T. P.; FARIA, O. A. C.; FIDELIS, A. A. G.; DODE, M. A. N.; VARGAS, L. N.; FIGUEIREDO, R. A. Features and development potential of oocytes collected from Nelore (Bos tuarus indicus) calves and the early and late prepubertal phase. Reproduction, Fertility and Development, v. 34, n. 2, p. 302, 2022 Edição dos Proceedings of the 48th International Embryo Technology Society Annual Meeting, Savannah, 2022.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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11. | | DODE, M. A. N.; CAIXETA, F. M. C.; VARGAS, L. N.; LEME, L. O.; KAWAMOTO, T. S.; FIDELIS, A. A. G.; FRANCO, M. M. Genome transfer technique for bovine embryo production using the metaphase plate and polar body. Journal of Assisted Reproduction and Genetics, v. 40, p. 943-951, 2023.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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12. | | KAWAMOTO, T. S.; PONTELO, T. P.; FARIA, O. A. C. de; FIDELIS, A. A. G.; DODE, M. A. N.; VIANA, J. H. M.; FIGUEIREDO, R. A. OPU and IVEP from non-stimulated 2-4 and 8-10 months old Nelore (Bos taurus indicus) donors. Animal Reproduction v.16, n.3, p.597, Jul./Sept. 2019. Edição dos Proceedings of the 33nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Ilha da Comandatuba, BA, Brazil, August 15th to 19th, 2019.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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13. | | PONTELO, T. P.; RODRIGUES, S. A. D.; KAWAMOTO, T. S.; LEME, L. O.; GOMES, A. C. M. M.; ZANGERONIMO, M. G.; FRANCO, M. M.; DODE, M. A. N. Histone acetylation during the in vitro maturation of bovine oocytes with different levels of competence. Reproduction, Fertility and Development, v. 32, n. 7, p. 690-696, 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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14. | | PONTELO, T. P.; FRANCO, M. M.; KAWAMOTO, T. S.; CAIXETA, F. M. C.; LEME, L. de O.; KUSSANO, N. R.; ZANGERONIMO, M. G.; DODE, M. A. N. Histone deacetylase inhibitor during in vitro maturation decreases developmental capacity of bovine oocytes. PLoS One, v. 16, n. 3, e0247518, 2021.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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15. | | KAWAMOTO, T. S.; VIANA, J. H. M.; PONTELO, T. P.; FRANCO, M. M.; FARIA, O. A. C. de; FIDELIS, A. A. G.; VARGAS, L. N.; FIGUEIREDO, R. A. Dynamics of the reproductive changes and acquisition of Oocyte competence in Nelore (Bos taurus indicus) Calves during the early and intermediate prepubertal periods. Animals, v. 12, n. 15, 2022. 2137.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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16. | | PONTELO, T. P.; RODRIGUES, S. de A. D.; KAWAMOTO, T. S.; LEME, L. O.; ZANGERONIMO, M. G.; FRANCO, M. M.; DODE, M. A. N. Transcripts levels of enzymes involved in histone acetylation in bovine oocytes of different competences. Animal Reprodroduction, v. 14, n. 3, p. 892, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. AbstractsTipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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17. | | FERNANDES, G. de O.; MILAZZOTTO, M. P.; FIDELIS, A. A. G.; KAWAMOTO, T. S.; LEME, L. de O.; LIMA, C. B. de; FRANCO, M. M.; DODE, M. A. N. Biochemical markers for pregnancy in the spent culture medium of in vitro produced bovine embryos. Biology of Reproduction, v. 105, n. 2, 481-490, 2021.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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18. | | RODRIGUES, S. A. D.; PONTELO, T. P.; KUSSANO, N. R.; KAWAMOTO, T. S.; LEME, L. O.; CAIXETA, F. M. C.; PFEIFER, L. F. M.; FRANCO, M. M.; DODE, M. A. N. Effects of Prostaglandins E2 and F2alpha on the in vitro maturation of bovine oocytes. Domestic Animal Endocrinology, v. 72, article 106447, 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Rondônia. |
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19. | | FARIA, O. A. C.; KAWAMOTO, T. S.; DIAS, L. R. O.; FIDELIS, A. A. G.; LEME, L. O.; CAIXETA, F. M. C.; GOMES, A. C. M. M.; SPRÍCIGO, J. F. W.; DODE, M. A. N. Maturation system affects lipid accumulation in bovine oocytes. Reproduction, Fertility and Development, v. 33, n. 5, 372-380, 2021.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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20. | | AMORIM, L. S.; KAWAMOTO, T. S.; TORRES, C. A. A.; GUIMARÃES, J. D.; SILVA FILHO, J. M.; OLIVEIRA, M. M. N. F.; CARVALHO, G. R.; FONSECA, J. F. da. Influência do Hormônio do Crescimento na concentração de testosterona plasmática e nas características seminais de touros jovens e adultos da raça Nelore. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 67, n. 1, p. 7-14, Fev. 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registros recuperados : 20 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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