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Registro Completo |
Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
01/03/1999 |
Data da última atualização: |
01/03/1999 |
Autoria: |
TAKKAR, P. N.; KAUR, N. P. |
Afiliação: |
Department of Soils, Punjab Agricultural University, Ludhiana, India. |
Título: |
HC1 method for Fe2+ estimation to resolve iron chlorosis in plants. |
Ano de publicação: |
1984 |
Fonte/Imprenta: |
Journal of Plant Nutrition, v.7, n.1/5, p.81-90, 1984. |
Idioma: |
Inglês |
Conteúdo: |
The equation dictates that acid solutions should not cause reduction of Fe3+ during extraction of Fe2+ from plants and provide the possibility to preferentially extract Fe2+. Both in vitro and in vivo extraction of Fe2+ with 1N HCl did not cause reduction of Fe3+. Two g chopped fresh plant was reacted with 0.5 to 1.5N HCl and 1:5 to 1:10 tissue: extractant ratios for 24 h. The Fe2+ and Fe3+ in buffered filterates (pH 3.0) were determined with 1-10 o-phenanthroline (o-Ph). 1N HCl and 1:10 ratio resolved better the Fe2+ differences between green and iron-chlorotic plants. Fe2+ iron in chlorotic as compared to non-chlorotic leaves of different crops was 30 to 82%. This method resolved better or equally good, the visible iron chlorosis in plants, compared to o-Ph method and is much cheaper. The method also eliminates the inherent error arising from extraction of pigments. |
Palavras-Chave: |
Acid fraction; Active iron; Ferro ativo; Ferroin; Fracao acida; In vivo; Invitro; Iron chlorosis; Reduction. |
Thesagro: |
Redução. |
Thesaurus Nal: |
chlorophyll. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01551naa a2200265 a 4500 001 1793802 005 1999-03-01 008 1984 bl --- 0-- u #d 100 1 $aTAKKAR, P. N. 245 $aHC1 method for Fe2+ estimation to resolve iron chlorosis in plants. 260 $c1984 520 $aThe equation dictates that acid solutions should not cause reduction of Fe3+ during extraction of Fe2+ from plants and provide the possibility to preferentially extract Fe2+. Both in vitro and in vivo extraction of Fe2+ with 1N HCl did not cause reduction of Fe3+. Two g chopped fresh plant was reacted with 0.5 to 1.5N HCl and 1:5 to 1:10 tissue: extractant ratios for 24 h. The Fe2+ and Fe3+ in buffered filterates (pH 3.0) were determined with 1-10 o-phenanthroline (o-Ph). 1N HCl and 1:10 ratio resolved better the Fe2+ differences between green and iron-chlorotic plants. Fe2+ iron in chlorotic as compared to non-chlorotic leaves of different crops was 30 to 82%. This method resolved better or equally good, the visible iron chlorosis in plants, compared to o-Ph method and is much cheaper. The method also eliminates the inherent error arising from extraction of pigments. 650 $achlorophyll 650 $aRedução 653 $aAcid fraction 653 $aActive iron 653 $aFerro ativo 653 $aFerroin 653 $aFracao acida 653 $aIn vivo 653 $aInvitro 653 $aIron chlorosis 653 $aReduction 700 1 $aKAUR, N. P. 773 $tJournal of Plant Nutrition$gv.7, n.1/5, p.81-90, 1984.
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Embrapa Pantanal (CPAP) |
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1. | | CHEN, W.; HASEGAWA, D. K.; KAUR, N.; KLIOT, A.; PINHEIRO, P. V.; LUAN, J.; STENSMYR, M. C.; ZHENG, Y.; LIU, W.; SUN, H.; XU, Y.; LUO, Y.; KRUSE, A.; YANG, X.; KONTSEDALOV, S.; LEBEDEV, G.; FISHER, T. W.; NELSON, D. R.; HUNTER, W. B.; BROWN, J. K.; JANDER, G.; CILIA, M.; DOUGLAS, A. E.; GHANIM, M.; SIMMONS, A. M.; WINTERMANTEL, W. M.; LING, K.-S.; FEI, Z. The draft genome of whitefly Bemisia tabaci MEAM1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance. BMC Biology, v. 14, p. 1-15, 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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