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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
15/03/2021 |
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Data da última atualização: |
10/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BOARI, A. de J.; QUADROS, A. F. F.; KAUFFMANN, C. M.; PANTOJA, K. F. C.; GOMES JUNIOR, R. A.; FREIRE FILHO, F. R.; OLIVEIRA, R. P. de; CORDOVIL, G. A.; GAVINHO, B. E. S.; KITAJIMA, E. W.; BLAWID, R.; NAGATA, T. |
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Afiliação: |
ALESSANDRA DE JESUS BOARI, CPATU; AYANE F. F. QUADROS, UFRA; CATERYNNE M. KAUFFMANN, UFRA; KÉSSIA F. C. PANTOJA, Univesidade Estadual de São Paulo; RUI ALBERTO GOMES JUNIOR, CPATU; FRANCISCO RODRIGUES FREIRE FILHO, CPATU; RAIMUNDO PARENTE DE OLIVEIRA, CPATU; GABRIELE A. CORDOVIL, UFRA; BRENDA E. S. GAVINHO, UFRA; ELLIOT W. KITAJIMA, ESALQ/USP; ROSANA BLAWID, Universidade Federal Rural de Pernambuco; TATSUYA NAGATA, UNB. |
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Título: |
Near-complete genome sequence of cowpea severe mosaic virusin South America and reduced yardlong bean production dueto the viral infection. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 46, n. 4, p. 487-491, Aug. 2021. |
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DOI: |
https://doi.org/10.1007/s40858-021-00420-w |
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Idioma: |
Inglês |
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Conteúdo: |
The sequence of a near-complete genome (two RNA segments) of cowpea severe mosaic virus (CpSMV) in the Comovirus genus is revealed in South America. High-throughput sequencing (HTS) was performed using total RNA extracted from partially purified virus preparations obtained from infected yardlong bean leaves (Vigna unguiculata subsp. sesquipedalis), collected in Santa Izabel, state of Pará, Brazil. The YLB (yardlong bean) isolate has an RNA-1 with 5896 nucleotides (nt) and an RNA-2 with 3515 nt, showing 74.2% and 67.9% identity, respectively, with the CpSMV-DG isolate sequences from the USA, the only complete genome available so far. Despite the low percentage in nt identity, the amino acid (aa) sequence of the viral protease together with the RNA-dependent RNA polymerase (Pro/RdRp) and the large and small coat proteins (CP-L/CP-S), used as species demarcation criteria of the Secoviridae family, showed 92.2% (Pro-RdRp) and 85.6% (CP-L/CL-S) identities between DG and YLB isolates sequences. The nucleotide identity of partial RNA-1 sequences of 2199 nt (part of the helicase, VPg, Pro, and 2/3 of the RdRP) varied from 95.1 to 95.3% among YLB and eleven isolates from Brazil available on GenBank. Phylogenetic analysis based on this 2199 nt sequence revealed that the YLB isolate was positioned in a different clade compared to other CpSMV isolates. An estimate of damage caused by CpSMV under experimental conditions revealed a reduction of 39.4% in the total number of pods and 54.3% in the total number of grains, regardless of the yardlong bean cultivar evaluated. The reduction of total weight of the grains by CpSMV infection was dependent on the cultivar and was higher in cv. De metro (66.2%) than in cv. Slin (38.9%). MenosThe sequence of a near-complete genome (two RNA segments) of cowpea severe mosaic virus (CpSMV) in the Comovirus genus is revealed in South America. High-throughput sequencing (HTS) was performed using total RNA extracted from partially purified virus preparations obtained from infected yardlong bean leaves (Vigna unguiculata subsp. sesquipedalis), collected in Santa Izabel, state of Pará, Brazil. The YLB (yardlong bean) isolate has an RNA-1 with 5896 nucleotides (nt) and an RNA-2 with 3515 nt, showing 74.2% and 67.9% identity, respectively, with the CpSMV-DG isolate sequences from the USA, the only complete genome available so far. Despite the low percentage in nt identity, the amino acid (aa) sequence of the viral protease together with the RNA-dependent RNA polymerase (Pro/RdRp) and the large and small coat proteins (CP-L/CP-S), used as species demarcation criteria of the Secoviridae family, showed 92.2% (Pro-RdRp) and 85.6% (CP-L/CL-S) identities between DG and YLB isolates sequences. The nucleotide identity of partial RNA-1 sequences of 2199 nt (part of the helicase, VPg, Pro, and 2/3 of the RdRP) varied from 95.1 to 95.3% among YLB and eleven isolates from Brazil available on GenBank. Phylogenetic analysis based on this 2199 nt sequence revealed that the YLB isolate was positioned in a different clade compared to other CpSMV isolates. An estimate of damage caused by CpSMV under experimental conditions revealed a reduction of 39.4% in the total number of pods and 54.3% ... Mostrar Tudo |
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Thesagro: |
Feijão; Genoma; Vigna Unguiculata; Vírus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02717naa a2200313 a 4500
001 2130679
005 2021-08-10
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s40858-021-00420-w$2DOI
100 1 $aBOARI, A. de J.
245 $aNear-complete genome sequence of cowpea severe mosaic virusin South America and reduced yardlong bean production dueto the viral infection.$h[electronic resource]
260 $c2021
520 $aThe sequence of a near-complete genome (two RNA segments) of cowpea severe mosaic virus (CpSMV) in the Comovirus genus is revealed in South America. High-throughput sequencing (HTS) was performed using total RNA extracted from partially purified virus preparations obtained from infected yardlong bean leaves (Vigna unguiculata subsp. sesquipedalis), collected in Santa Izabel, state of Pará, Brazil. The YLB (yardlong bean) isolate has an RNA-1 with 5896 nucleotides (nt) and an RNA-2 with 3515 nt, showing 74.2% and 67.9% identity, respectively, with the CpSMV-DG isolate sequences from the USA, the only complete genome available so far. Despite the low percentage in nt identity, the amino acid (aa) sequence of the viral protease together with the RNA-dependent RNA polymerase (Pro/RdRp) and the large and small coat proteins (CP-L/CP-S), used as species demarcation criteria of the Secoviridae family, showed 92.2% (Pro-RdRp) and 85.6% (CP-L/CL-S) identities between DG and YLB isolates sequences. The nucleotide identity of partial RNA-1 sequences of 2199 nt (part of the helicase, VPg, Pro, and 2/3 of the RdRP) varied from 95.1 to 95.3% among YLB and eleven isolates from Brazil available on GenBank. Phylogenetic analysis based on this 2199 nt sequence revealed that the YLB isolate was positioned in a different clade compared to other CpSMV isolates. An estimate of damage caused by CpSMV under experimental conditions revealed a reduction of 39.4% in the total number of pods and 54.3% in the total number of grains, regardless of the yardlong bean cultivar evaluated. The reduction of total weight of the grains by CpSMV infection was dependent on the cultivar and was higher in cv. De metro (66.2%) than in cv. Slin (38.9%).
650 $aFeijão
650 $aGenoma
650 $aVigna Unguiculata
650 $aVírus
700 1 $aQUADROS, A. F. F.
700 1 $aKAUFFMANN, C. M.
700 1 $aPANTOJA, K. F. C.
700 1 $aGOMES JUNIOR, R. A.
700 1 $aFREIRE FILHO, F. R.
700 1 $aOLIVEIRA, R. P. de
700 1 $aCORDOVIL, G. A.
700 1 $aGAVINHO, B. E. S.
700 1 $aKITAJIMA, E. W.
700 1 $aBLAWID, R.
700 1 $aNAGATA, T.
773 $tTropical Plant Pathology$gv. 46, n. 4, p. 487-491, Aug. 2021.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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Biblioteca |
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Origem |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Pecuária Sul. |
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Data corrente: |
08/02/2011 |
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Data da última atualização: |
09/06/2011 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
CUNHA, R. P. da; PERES, É. R.; MONTARDO, D. P.; MITTELMANN, A. |
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Afiliação: |
RICARDO PEREIRA DA CUNHA, Urcamp; ÉDER RODRIGUES PERES, Urcamp; DANIEL PORTELLA MONTARDO, CPPSUL; ANDREA MITTELMANN, CNPGL. |
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Título: |
Produção de forragem de populações de trevos anuais na região da Campanha do Rio Grande do Sul. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: ENCONTRO DE INICIAÇÃO CIENTÍFICA E PÓS GRADUAÇÃO DA EMBRAPA CLIMA TEMPERADO, 3., 2010, Pelotas. Carreira, ética e inovação: o que você está fazendo? resumos e palestras. Pelotas: Embrapa Clima Temperado, 2010. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Notas: |
Editado por Ivan Rodrigues de Almeida, Leonardo Ferreira Dutra e Jamir Luis Silva da Silva. |
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Palavras-Chave: |
Forrageira; Genero. |
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Thesagro: |
Forragem; Produção; Trevo; Trifolium. |
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Categoria do assunto: |
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K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/26602/1/metas-2010-dpm-0001.pdf
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Marc: |
LEADER 00911nam a2200229 a 4500
001 1876257
005 2011-06-09
008 2010 bl uuuu u00u1 u #d
100 1 $aCUNHA, R. P. da
245 $aProdução de forragem de populações de trevos anuais na região da Campanha do Rio Grande do Sul.$h[electronic resource]
260 $aIn: ENCONTRO DE INICIAÇÃO CIENTÍFICA E PÓS GRADUAÇÃO DA EMBRAPA CLIMA TEMPERADO, 3., 2010, Pelotas. Carreira, ética e inovação: o que você está fazendo? resumos e palestras. Pelotas: Embrapa Clima Temperado$c2010
300 $c1 CD-ROM.
500 $aEditado por Ivan Rodrigues de Almeida, Leonardo Ferreira Dutra e Jamir Luis Silva da Silva.
650 $aForragem
650 $aProdução
650 $aTrevo
650 $aTrifolium
653 $aForrageira
653 $aGenero
700 1 $aPERES, É. R.
700 1 $aMONTARDO, D. P.
700 1 $aMITTELMANN, A.
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Embrapa Pecuária Sul (CPPSUL) |
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