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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
26/03/2012 |
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Data da última atualização: |
26/01/2017 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
FALEIRO, F. G.; KANNAN, B.; ALTPETER, F. |
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Afiliação: |
FABIO GELAPE FALEIRO, CPAC; BASKARAN KANNAN; FREDY ALTPETER. |
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Título: |
In vitro chromossome doubling of superior interspecific hybrids between elephantgrass and pearl millet using different antimitotic chemicals. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: ANNUAL FESC SUMMIT, 3., 2011, Florida. Oral and poster session: abstracts. [Gainesville: FESC, 2011]. |
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Páginas: |
p. 26. |
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Idioma: |
Inglês |
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Notas: |
Florida Energy Systems Consortium. |
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Conteúdo: |
Elephantgrass (Pennisetum purpureum 2n = 4x = 28) produces large amounts of biomass in tropical and subtropical regions and is considered a prime candidate for production of lignocellulosic biofuel. Interspecific hybridization between elephantgrass and pearl millet (Pennisetum glaucum 2n = 2x = 14) may allow to improve important traits (e.g. drought tolerance, biomass quality and production). In contrast to elephantgrass and pearl millet, which produce large amounts of seeds, interspecific hybrids are male and female sterile due to triploidy (2n = 3x = 21) and do not produce seeds. Chromosome doubling of the triploid hybrids may restore seed production and fertility, allowing backcrosses with elephantgrass to further enhance biomass production and persistence. In this study, chromosome doubling was performed in vitro from the most persistent and productive interspecific hybrids. Callus was induced in vitro from cross-sections of the immature leaf whorl of five superior interspecific hybrids (MN 12, MN 18, MN 51, MN 54 and MN 55). Embryogenic calli were incubated for 48h with two alternative concentrations of the antimitotic agents Oryzalin or Trifluralin. Stomata size measurements and flow cytometry were performed following plant regeneration from embryogenic callus and transfer to soil. A total of 328 regenerated plants were obtained and 74 presented altered ploidy according to flow cytometer analysis. Stomata longitudinal diameter was a good indicator of altered ploidy. The treatment with 5 µM Oryzalin produced the highest number (55) of plants with altered ploidy. Chromosome doubling was obtained for all five superior interspecific hybrids. The highest numbers of plants with altered ploidy was obtained from MN 18 and MN 51 genotypes with of 29 and 27 plants, respectively. The plants with altered ploidy have been transferred to the field to evaluate agronomic performance and fertility. The best performing plants with recovered fertility will be used in further elephant grass breeding cycles. MenosElephantgrass (Pennisetum purpureum 2n = 4x = 28) produces large amounts of biomass in tropical and subtropical regions and is considered a prime candidate for production of lignocellulosic biofuel. Interspecific hybridization between elephantgrass and pearl millet (Pennisetum glaucum 2n = 2x = 14) may allow to improve important traits (e.g. drought tolerance, biomass quality and production). In contrast to elephantgrass and pearl millet, which produce large amounts of seeds, interspecific hybrids are male and female sterile due to triploidy (2n = 3x = 21) and do not produce seeds. Chromosome doubling of the triploid hybrids may restore seed production and fertility, allowing backcrosses with elephantgrass to further enhance biomass production and persistence. In this study, chromosome doubling was performed in vitro from the most persistent and productive interspecific hybrids. Callus was induced in vitro from cross-sections of the immature leaf whorl of five superior interspecific hybrids (MN 12, MN 18, MN 51, MN 54 and MN 55). Embryogenic calli were incubated for 48h with two alternative concentrations of the antimitotic agents Oryzalin or Trifluralin. Stomata size measurements and flow cytometry were performed following plant regeneration from embryogenic callus and transfer to soil. A total of 328 regenerated plants were obtained and 74 presented altered ploidy according to flow cytometer analysis. Stomata longitudinal diameter was a good indicator of altered ploidy. Th... Mostrar Tudo |
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Thesagro: |
Pennisetum Purpureum. |
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Categoria do assunto: |
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Marc: |
LEADER 02667naa a2200181 a 4500
001 1920322
005 2017-01-26
008 2011 bl uuuu u00u1 u #d
100 1 $aFALEIRO, F. G.
245 $aIn vitro chromossome doubling of superior interspecific hybrids between elephantgrass and pearl millet using different antimitotic chemicals.
260 $c2011
300 $ap. 26.
500 $aFlorida Energy Systems Consortium.
520 $aElephantgrass (Pennisetum purpureum 2n = 4x = 28) produces large amounts of biomass in tropical and subtropical regions and is considered a prime candidate for production of lignocellulosic biofuel. Interspecific hybridization between elephantgrass and pearl millet (Pennisetum glaucum 2n = 2x = 14) may allow to improve important traits (e.g. drought tolerance, biomass quality and production). In contrast to elephantgrass and pearl millet, which produce large amounts of seeds, interspecific hybrids are male and female sterile due to triploidy (2n = 3x = 21) and do not produce seeds. Chromosome doubling of the triploid hybrids may restore seed production and fertility, allowing backcrosses with elephantgrass to further enhance biomass production and persistence. In this study, chromosome doubling was performed in vitro from the most persistent and productive interspecific hybrids. Callus was induced in vitro from cross-sections of the immature leaf whorl of five superior interspecific hybrids (MN 12, MN 18, MN 51, MN 54 and MN 55). Embryogenic calli were incubated for 48h with two alternative concentrations of the antimitotic agents Oryzalin or Trifluralin. Stomata size measurements and flow cytometry were performed following plant regeneration from embryogenic callus and transfer to soil. A total of 328 regenerated plants were obtained and 74 presented altered ploidy according to flow cytometer analysis. Stomata longitudinal diameter was a good indicator of altered ploidy. The treatment with 5 µM Oryzalin produced the highest number (55) of plants with altered ploidy. Chromosome doubling was obtained for all five superior interspecific hybrids. The highest numbers of plants with altered ploidy was obtained from MN 18 and MN 51 genotypes with of 29 and 27 plants, respectively. The plants with altered ploidy have been transferred to the field to evaluate agronomic performance and fertility. The best performing plants with recovered fertility will be used in further elephant grass breeding cycles.
650 $aPennisetum Purpureum
700 1 $aKANNAN, B.
700 1 $aALTPETER, F.
773 $tIn: ANNUAL FESC SUMMIT, 3., 2011, Florida. Oral and poster session: abstracts. [Gainesville: FESC, 2011].
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Embrapa Cerrados (CPAC) |
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| Registros recuperados : 4 | |
| 2. |  | FALEIRO, F. G.; KANNAN, B.; ALTPETER, F. Regeneration of fertile, hexaploid, interspecific hybrids of elephantgrass and pearl millet following treatment of embryogenic calli with antimitotic agents. Plant Cell, Tissue and Organ Culture, v. 124, n. 1, p. 57-67, January 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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| Registros recuperados : 4 | |
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