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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
15/04/1997 |
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Data da última atualização: |
15/04/1997 |
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Autoria: |
GONCALVES-VIDIGAL, M. C.; VILARINHOS, A. D.; BARROS. E. G. de; PAULA JUNIOR, T. T.; Cruz, C. D.; MOREIRA, M. A. |
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Afiliação: |
Universidade Estadual de Maringa - Departamento de Agronomia; UFV; EPAMIG. |
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Título: |
Variabilidade genetica entre variedades de feijoeiro (Phaseolus vulgaris L.) diferenciadoras de Colletotrichum lindemuthianum por meio de marcadores moleculares RAPD |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Goiania, GO: EMBRAPA-CNPAF-APA, 1996. |
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Páginas: |
p.230-232 |
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Série: |
EMBRAPA-CNPAF. Documentos, 69 |
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ISSN: |
0101-9716 |
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Idioma: |
Português |
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Notas: |
In: REUNIAO NACIONAL DE PESQUISA DE FEIJAO, 5., 1996, Goiania, GO. Anais... Goiania, GO: EMBRAPA-CNPAF, 1996. |
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Conteúdo: |
A antracnose do feijoeiro (Phaseolus vulgaris L.), causada pelo fungo Colletotrichum lindemuthianum Scrib., e uma das doencas de maior importancia da cultura, que causa serias perdas quando cultivares suscetiveis sao estabelecidos em ambientes frios e umidos. A classificacao de novos isolamentos de Colletotrichum lindemuthianum e usualmente realizada analisando a resposta dos cultivos diferenciadores ao isolamento. O uso de marcadores moleculares para a caracterizacao e para o estudo da divergencia genetica em plantas tem sido comum nos programas de melhoramento de diversas especies cultivadas. Neste trabalho foi estudada a relacao genetica entre os cultivares diferenciadores, Michelite, Dark Red Kidney, Perry Marrow, Cornell 49242, Widusa, Kaboon, Mexico 222, PI 207262, To, Tu, AB 136 e G 2333, utilizando a tecnica do DNA polimorfico amplificado ao acaso (RAPD). A extracao do DNA total seguiu a metodologia proposta por Saghhhai-Maroof et al. (Proc. Nat. Acad. Sci., USA, v.81, p.8014-8018, 1984). O DNA extraido dos diferentes genotipos foi usado como molde para reacoes de amplificacao. Cada reacao (25 uL) continha: 50ng de DNA; 100uM de cada um dos desoxirribonucleotideos (dATP, dCTP, dGTP e dTTP); 1,7mM de MgCl2; 10mM de Tris-HCl, pH 8,3; 50mM de KCl; 0,4uM do iniciador (Operon Technologies); 1 unidade de Taq polimerase (Perkin Elmer-Cetus Corp.). As reacoes de amplificacao foram efetuadas no termociclador Perkin Elmer modelo 9600. |
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Palavras-Chave: |
Bean; Funcao; Fungus; Genetic. |
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Thesagro: |
Antracnose; Colletotrichum Lindemuthianum; Feijão; Genética; Phaseolus Vulgaris. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02528naa a2200337 a 4500
001 1647824
005 1997-04-15
008 1996 bl --- 0-- u #d
022 $a0101-9716
100 1 $aGONCALVES-VIDIGAL, M. C.
245 $aVariabilidade genetica entre variedades de feijoeiro (Phaseolus vulgaris L.) diferenciadoras de Colletotrichum lindemuthianum por meio de marcadores moleculares RAPD
260 $c1996
300 $ap.230-232
490 $aEMBRAPA-CNPAF. Documentos, 69
500 $aIn: REUNIAO NACIONAL DE PESQUISA DE FEIJAO, 5., 1996, Goiania, GO. Anais... Goiania, GO: EMBRAPA-CNPAF, 1996.
520 $aA antracnose do feijoeiro (Phaseolus vulgaris L.), causada pelo fungo Colletotrichum lindemuthianum Scrib., e uma das doencas de maior importancia da cultura, que causa serias perdas quando cultivares suscetiveis sao estabelecidos em ambientes frios e umidos. A classificacao de novos isolamentos de Colletotrichum lindemuthianum e usualmente realizada analisando a resposta dos cultivos diferenciadores ao isolamento. O uso de marcadores moleculares para a caracterizacao e para o estudo da divergencia genetica em plantas tem sido comum nos programas de melhoramento de diversas especies cultivadas. Neste trabalho foi estudada a relacao genetica entre os cultivares diferenciadores, Michelite, Dark Red Kidney, Perry Marrow, Cornell 49242, Widusa, Kaboon, Mexico 222, PI 207262, To, Tu, AB 136 e G 2333, utilizando a tecnica do DNA polimorfico amplificado ao acaso (RAPD). A extracao do DNA total seguiu a metodologia proposta por Saghhhai-Maroof et al. (Proc. Nat. Acad. Sci., USA, v.81, p.8014-8018, 1984). O DNA extraido dos diferentes genotipos foi usado como molde para reacoes de amplificacao. Cada reacao (25 uL) continha: 50ng de DNA; 100uM de cada um dos desoxirribonucleotideos (dATP, dCTP, dGTP e dTTP); 1,7mM de MgCl2; 10mM de Tris-HCl, pH 8,3; 50mM de KCl; 0,4uM do iniciador (Operon Technologies); 1 unidade de Taq polimerase (Perkin Elmer-Cetus Corp.). As reacoes de amplificacao foram efetuadas no termociclador Perkin Elmer modelo 9600.
650 $aAntracnose
650 $aColletotrichum Lindemuthianum
650 $aFeijão
650 $aGenética
650 $aPhaseolus Vulgaris
653 $aBean
653 $aFuncao
653 $aFungus
653 $aGenetic
700 1 $aVILARINHOS, A. D.
700 1 $aBARROS. E. G. de
700 1 $aPAULA JUNIOR, T. T.
700 1 $aCruz, C. D.
700 1 $aMOREIRA, M. A.
773 $tGoiania, GO: EMBRAPA-CNPAF-APA, 1996.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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Origem |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
29/12/2015 |
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Data da última atualização: |
03/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SOUZA, K. C. de; ANDRIOLI, A.; SIDER, L. H.; PINHEIRO, R. R.; BEZERRA JUNIOR, R. Q.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S. |
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Afiliação: |
Kelma Costa de Souza, Pós-graduação - Universidade Estadual do Ceará (UECE) - Fortaleza, CE; ALICE ANDRIOLI, CNPC; LUCIA HELENA SIDER, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; Rosivaldo Quirino Bezerra Junior; Renato Mesquita Peixoto; Maria Fátima da Silva Teixeira. |
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Título: |
Detecção de sequências do DNA proviral do vírus da Artrite Encefalite Caprina em saliva. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 43, n. 1266, p. 1-6, 2015. |
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Idioma: |
Português |
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Conteúdo: |
[Detection of Proviral Sequences of the Caprine Arthritis Encephalitis Virus in Saliva]. Background: Goats can be infected by a lentivirus called the caprine arthritis encephalitis virus (CAEV) that causes an infectious disease characterized by a chronic. For replication, the CAEV, integrate as a provirus in the DNA of the host cell genome. By consequence, infection of cells persistent life-long infection of the animal. The CAEV can be found in most body fluids, It has been demonstrated its presence in blood, milk, semen. However, the search for the CAEV in other body fluids besides blood is important to assess possible viral transmission. The aim of this study was to determine whether the presence of proviral DNA sequences in saliva of animals infected. Materials, Methods & Results: The study was carried out on the farm belonging to the Embrapa Goat and Sheep Research Center, located in the municipality of Sobral, Ceará, Brazil. To assess the oral fluid for the presence of CAEV, samples of saliva from eight infected breeders were collected by suctioning saliva from the oral cavity on the side region of the breeders lower molar teeth using a probe coupled to a plastic 5 mL syringe. And pro-viral DNA was extracted from the samples using NaCl and proteinase K. Two rounds of polymerase chain reaction (nested PCR) were carried out to amplify the final 187 pb fragment of the pro-viral DNA. All the oligonucleotide primers were determined from the gag region of the structural gene of the CAEV-Cork. The fragments amplified by PCR were analyzed in electrophoresis in a horizontal cube. On 2% agarose gel and stained with ethidium bromide (10?g/?L). The products of the PCR ampli?cation were sequenced in platform Applied Biosystems, the sequences were compared to available GenBank strains, and the results of this comparison demonstrated that were more related to the caprine strains regional. The presence of pro-viral DNA was observed in saliva in of 100% animals and proved that saliva of infected goats contained the CAEV. However, when assessing the results of CAEV pro-viral DNA detection in the present study, it was observed variation in the detection frequency of lentivirus in samples collected at different times. Discussion: This finding indicated that the CAEV was present in saliva samples in its pro-viral form, a factor that would not limit its contagion capacity and that direct, continued exposure in a favorable environment presents perfect conditions for its transmission by this pathway. The results found in the present study corroborated studies on other lentivirus. Isolated the maedi-visna virus (MVV) in saliva glands of experimentally infected sheep. In cats, the feline immunodeficiency virus (FIV) was also detected in the saliva of infected animals and transmission was reported. In a recent study, was detected the presence of the lentivirus in small ruminants by PCR in water and air samples in different installations that housed herds with medium to high infection prevalence. Throughout in this study, an intermitency in proviral elimination was observed in saliva similar blood and semen, as reported by other authors for CAEV and other virus. Although the determining mechanism of horizontal transmission through the oral fluids was not definitively elucidated, these studies highlight the risks of transmission through direct contact with animals or indirect contamination by food and/or water in the drinker. This study proved that saliva from infected goats contains the CAEV, more studies should be carried out to analyze the degree of infectiousness of the virus in the saliva and the viral elimination characteristics in this medium. Menos[Detection of Proviral Sequences of the Caprine Arthritis Encephalitis Virus in Saliva]. Background: Goats can be infected by a lentivirus called the caprine arthritis encephalitis virus (CAEV) that causes an infectious disease characterized by a chronic. For replication, the CAEV, integrate as a provirus in the DNA of the host cell genome. By consequence, infection of cells persistent life-long infection of the animal. The CAEV can be found in most body fluids, It has been demonstrated its presence in blood, milk, semen. However, the search for the CAEV in other body fluids besides blood is important to assess possible viral transmission. The aim of this study was to determine whether the presence of proviral DNA sequences in saliva of animals infected. Materials, Methods & Results: The study was carried out on the farm belonging to the Embrapa Goat and Sheep Research Center, located in the municipality of Sobral, Ceará, Brazil. To assess the oral fluid for the presence of CAEV, samples of saliva from eight infected breeders were collected by suctioning saliva from the oral cavity on the side region of the breeders lower molar teeth using a probe coupled to a plastic 5 mL syringe. And pro-viral DNA was extracted from the samples using NaCl and proteinase K. Two rounds of polymerase chain reaction (nested PCR) were carried out to amplify the final 187 pb fragment of the pro-viral DNA. All the oligonucleotide primers were determined from the gag region of the structural gene ... Mostrar Tudo |
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Palavras-Chave: |
Artrite-encefalite Caprina; CAEV; Caprine arthritis encephalit virus; DNA sequence; PCR. |
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Thesagro: |
Caprino; Doença animal; Saliva; Transmissão de doença; Vírus. |
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Thesaurus NAL: |
Animal diseases; Disease transmission; Goats. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/136302/1/cnpc-2015-Deteccao.pdf
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Marc: |
LEADER 04687naa a2200349 a 4500
001 2032574
005 2019-04-03
008 2015 bl uuuu u00u1 u #d
100 1 $aSOUZA, K. C. de
245 $aDetecção de sequências do DNA proviral do vírus da Artrite Encefalite Caprina em saliva.$h[electronic resource]
260 $c2015
520 $a[Detection of Proviral Sequences of the Caprine Arthritis Encephalitis Virus in Saliva]. Background: Goats can be infected by a lentivirus called the caprine arthritis encephalitis virus (CAEV) that causes an infectious disease characterized by a chronic. For replication, the CAEV, integrate as a provirus in the DNA of the host cell genome. By consequence, infection of cells persistent life-long infection of the animal. The CAEV can be found in most body fluids, It has been demonstrated its presence in blood, milk, semen. However, the search for the CAEV in other body fluids besides blood is important to assess possible viral transmission. The aim of this study was to determine whether the presence of proviral DNA sequences in saliva of animals infected. Materials, Methods & Results: The study was carried out on the farm belonging to the Embrapa Goat and Sheep Research Center, located in the municipality of Sobral, Ceará, Brazil. To assess the oral fluid for the presence of CAEV, samples of saliva from eight infected breeders were collected by suctioning saliva from the oral cavity on the side region of the breeders lower molar teeth using a probe coupled to a plastic 5 mL syringe. And pro-viral DNA was extracted from the samples using NaCl and proteinase K. Two rounds of polymerase chain reaction (nested PCR) were carried out to amplify the final 187 pb fragment of the pro-viral DNA. All the oligonucleotide primers were determined from the gag region of the structural gene of the CAEV-Cork. The fragments amplified by PCR were analyzed in electrophoresis in a horizontal cube. On 2% agarose gel and stained with ethidium bromide (10?g/?L). The products of the PCR ampli?cation were sequenced in platform Applied Biosystems, the sequences were compared to available GenBank strains, and the results of this comparison demonstrated that were more related to the caprine strains regional. The presence of pro-viral DNA was observed in saliva in of 100% animals and proved that saliva of infected goats contained the CAEV. However, when assessing the results of CAEV pro-viral DNA detection in the present study, it was observed variation in the detection frequency of lentivirus in samples collected at different times. Discussion: This finding indicated that the CAEV was present in saliva samples in its pro-viral form, a factor that would not limit its contagion capacity and that direct, continued exposure in a favorable environment presents perfect conditions for its transmission by this pathway. The results found in the present study corroborated studies on other lentivirus. Isolated the maedi-visna virus (MVV) in saliva glands of experimentally infected sheep. In cats, the feline immunodeficiency virus (FIV) was also detected in the saliva of infected animals and transmission was reported. In a recent study, was detected the presence of the lentivirus in small ruminants by PCR in water and air samples in different installations that housed herds with medium to high infection prevalence. Throughout in this study, an intermitency in proviral elimination was observed in saliva similar blood and semen, as reported by other authors for CAEV and other virus. Although the determining mechanism of horizontal transmission through the oral fluids was not definitively elucidated, these studies highlight the risks of transmission through direct contact with animals or indirect contamination by food and/or water in the drinker. This study proved that saliva from infected goats contains the CAEV, more studies should be carried out to analyze the degree of infectiousness of the virus in the saliva and the viral elimination characteristics in this medium.
650 $aAnimal diseases
650 $aDisease transmission
650 $aGoats
650 $aCaprino
650 $aDoença animal
650 $aSaliva
650 $aTransmissão de doença
650 $aVírus
653 $aArtrite-encefalite Caprina
653 $aCAEV
653 $aCaprine arthritis encephalit virus
653 $aDNA sequence
653 $aPCR
700 1 $aANDRIOLI, A.
700 1 $aSIDER, L. H.
700 1 $aPINHEIRO, R. R.
700 1 $aBEZERRA JUNIOR, R. Q.
700 1 $aPEIXOTO, R. M.
700 1 $aTEIXEIRA, M. F. da S.
773 $tActa Scientiae Veterinariae$gv. 43, n. 1266, p. 1-6, 2015.
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Embrapa Caprinos e Ovinos (CNPC) |
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