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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Agropecuária Oeste; Embrapa Hortaliças; Embrapa Meio-Norte. |
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Data corrente: |
25/08/2004 |
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Data da última atualização: |
25/08/2004 |
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Autoria: |
CHALFUN JUNIOR, A. |
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Título: |
Molecular analysis of plant architecture in Arabidopsis thaliana using activation tagging. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
2004. |
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Páginas: |
144 p. |
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Idioma: |
Inglês |
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Notas: |
Thesis (Doctor)- Wageningen Universiteit, Wageningen, 2004. |
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Conteúdo: |
Activation and suppression; getting insights in regulatory switches controlling plant development. Analysis of the SHP2 enhancer for the use of tissue specific activation tagging in Arabidopsis thaliana. Low frequency of T-DNA based activation tagging in Arabidopsis is correlated with methylation of CaMV35S enhancer sequences. Downwards siliquesi, a member of the lateral organ boundary family, controls petiole-blade boundary in Arabidopsis petals. Needlei, a Helix-Loop-Helix transcription factor involved in Arabidopsis carpel development. Overexpression of the Arabidopsis Twistedi gene affects plant architecture.
Plant development is one of the most important aspects of plant's life cycle that has extensively been studied at the morphologiacal, genetic and molecular level. It is import for systematic and taxonomic classification, but also for applied agronomic reasons, because it affects the growth and cultivation leading to higher yield and quality of the product. The generation of genetc variants, like mutants may increase genetic pool and gives information about plant processes and their genetic control. Activation tagging is a new poewrful tool to generate and identify new mutants, which emerged as an alternative for gene function analysis. This thesis reports the study on the molecular control of plant architecture, using mutants generated by an activation tagging-based approach in the model plat Arabidopsis thaliana. In addition, it also describes experiments that could explain why tha low frequencies of mutants were obtained by T-DNA based activation tagging. Based on this comparison, the transposon-based activation tagging strategy was chosen and a screen for flower and silique in a large Arabidopsis population yielded three gain-of-function mutants. These mutants were designated downwards siliques 1 (ds 1-D), needlel (ndl1-D) and twisted 1 (twt 1-D).in the ds 1-D mutant, internodes are shorter and the lateral organs such as flowers are bending downwards. Further molecular and genetic studies on this mutant evealed that DS 1 is important to control petiole-blade boundary in Arabidopsis petals. In the ndl 1-D mutant, the normal formation of valve tissues is altered, resulting in a pin-like structure that replaces the two carpels of the wild type pistil. The results suggest that suggest that NDL 1 is involved in normail carpel development, in which auxin distribution plays an important role. In the third mutant, twt 1-D, the overexpression of TWT 1 led to twisting of all organs, which is most pronounced in siliques. This phenotype and the expression pattern of the gene suggest that TWT 1 is involve in prope vascular tissue development in Arabidopsis. These studies demonstrate the power of activation tagging and it gains valuable knowledge about the molecular networks that control plant development. MenosActivation and suppression; getting insights in regulatory switches controlling plant development. Analysis of the SHP2 enhancer for the use of tissue specific activation tagging in Arabidopsis thaliana. Low frequency of T-DNA based activation tagging in Arabidopsis is correlated with methylation of CaMV35S enhancer sequences. Downwards siliquesi, a member of the lateral organ boundary family, controls petiole-blade boundary in Arabidopsis petals. Needlei, a Helix-Loop-Helix transcription factor involved in Arabidopsis carpel development. Overexpression of the Arabidopsis Twistedi gene affects plant architecture.
Plant development is one of the most important aspects of plant's life cycle that has extensively been studied at the morphologiacal, genetic and molecular level. It is import for systematic and taxonomic classification, but also for applied agronomic reasons, because it affects the growth and cultivation leading to higher yield and quality of the product. The generation of genetc variants, like mutants may increase genetic pool and gives information about plant processes and their genetic control. Activation tagging is a new poewrful tool to generate and identify new mutants, which emerged as an alternative for gene function analysis. This thesis reports the study on the molecular control of plant architecture, using mutants generated by an activation tagging-based approach in the model plat Arabidopsis thaliana. In addition, it also describes experiments that ... Mostrar Tudo |
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Palavras-Chave: |
Ativação genética marcada; Ativação géntica marcada; Classificação botânica; Controle molecular; Cultivo; Desenvolvimento; T - DNA; T DNA. |
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Thesagro: |
Arquitetura; Erva Daninha; Mutante; Planta; Produtividade; Qualidade; Taxonomia Vegetal. |
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Thesaurus Nal: |
Arabidopsis thaliana. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03767nam a2200325 a 4500
001 1248550
005 2004-08-25
008 2004 bl uuuu m 00u1 u #d
100 1 $aCHALFUN JUNIOR, A.
245 $aMolecular analysis of plant architecture in Arabidopsis thaliana using activation tagging.
260 $a2004.$c2004
300 $a144 p.
500 $aThesis (Doctor)- Wageningen Universiteit, Wageningen, 2004.
520 $aActivation and suppression; getting insights in regulatory switches controlling plant development. Analysis of the SHP2 enhancer for the use of tissue specific activation tagging in Arabidopsis thaliana. Low frequency of T-DNA based activation tagging in Arabidopsis is correlated with methylation of CaMV35S enhancer sequences. Downwards siliquesi, a member of the lateral organ boundary family, controls petiole-blade boundary in Arabidopsis petals. Needlei, a Helix-Loop-Helix transcription factor involved in Arabidopsis carpel development. Overexpression of the Arabidopsis Twistedi gene affects plant architecture. Plant development is one of the most important aspects of plant's life cycle that has extensively been studied at the morphologiacal, genetic and molecular level. It is import for systematic and taxonomic classification, but also for applied agronomic reasons, because it affects the growth and cultivation leading to higher yield and quality of the product. The generation of genetc variants, like mutants may increase genetic pool and gives information about plant processes and their genetic control. Activation tagging is a new poewrful tool to generate and identify new mutants, which emerged as an alternative for gene function analysis. This thesis reports the study on the molecular control of plant architecture, using mutants generated by an activation tagging-based approach in the model plat Arabidopsis thaliana. In addition, it also describes experiments that could explain why tha low frequencies of mutants were obtained by T-DNA based activation tagging. Based on this comparison, the transposon-based activation tagging strategy was chosen and a screen for flower and silique in a large Arabidopsis population yielded three gain-of-function mutants. These mutants were designated downwards siliques 1 (ds 1-D), needlel (ndl1-D) and twisted 1 (twt 1-D).in the ds 1-D mutant, internodes are shorter and the lateral organs such as flowers are bending downwards. Further molecular and genetic studies on this mutant evealed that DS 1 is important to control petiole-blade boundary in Arabidopsis petals. In the ndl 1-D mutant, the normal formation of valve tissues is altered, resulting in a pin-like structure that replaces the two carpels of the wild type pistil. The results suggest that suggest that NDL 1 is involved in normail carpel development, in which auxin distribution plays an important role. In the third mutant, twt 1-D, the overexpression of TWT 1 led to twisting of all organs, which is most pronounced in siliques. This phenotype and the expression pattern of the gene suggest that TWT 1 is involve in prope vascular tissue development in Arabidopsis. These studies demonstrate the power of activation tagging and it gains valuable knowledge about the molecular networks that control plant development.
650 $aArabidopsis thaliana
650 $aArquitetura
650 $aErva Daninha
650 $aMutante
650 $aPlanta
650 $aProdutividade
650 $aQualidade
650 $aTaxonomia Vegetal
653 $aAtivação genética marcada
653 $aAtivação géntica marcada
653 $aClassificação botânica
653 $aControle molecular
653 $aCultivo
653 $aDesenvolvimento
653 $aT - DNA
653 $aT DNA
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Registro original: |
Embrapa Agropecuária Oeste (CPAO) |
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| 81. |  | BATISTA, E. L.; MEDEIROS JÚNIOR, A. E.; ROCHA JÚNIOR, A. F.; MOUSINHO, F. E. P.; ANDRADE JÚNIOR, A. S. Variação no rendimento do feijão-caupi irrigado em função da uniformidade de distribuição de água. In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 2., 2009, Belém, PA. Da agricultura de subsistência ao agronegócio: anais. Belém, PA: Embrapa Amazônia Oriental, 2009. p. 901-905. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 99. | | AMABILE, R. F.; NASCIMENTO JUNIOR, A. do. Rendimento de grãos de triticale no Brasil Central. In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 2., 2008, Passo Fundo. Atas e resumos... Passo Fundo: Comissão Brasileira de Pesquisa de Trigo e Triticale: Embrapa Trigo: Embrapa Transferência de Tecnologia, 2008. 3 p. 1 CD-ROM. Melhoramento, 20. Área: Melhoramento, Aptidão Industrial e Sementes.| Tipo: Resumo em Anais de Congresso |
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