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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
15/04/2004 |
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Data da última atualização: |
15/04/2004 |
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Autoria: |
JORGE, M. A; ROBERTS, A. |
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Título: |
Factors affecting the acclimatization of ex-vitro cassava. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Revista Brasileira de Mandioca, Cruz das Almas, v.17, p.53, nov., 1998. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: In the semi-arid regions acclimatization process from tissue culture to the field is a problem compared to the humid tropics. The reasons are a long dry atmosphere and both diurnal and annual temperature range (from frost to 40°C). Previous data from trials of cassava (Manihot esculenta Crantz) in Mozambique, where IITA methods were compared to a locally adapted method, were not very successful, with 35% survial with adapted. IITA method. Studies to improve survial of ex vitro plantlets are still ongoing. Results are presented that include comparisons of light intensity levels in k-.he laboratory (64, 124, 205, 285, and 368 mEs-¹ m-²). Levels of fertilizer (NPK 94-253-51) (0,0.5,1,1-5 and 2.0g/
1), types of substrate, namely milled pine bark, vermiculite, and theree mixtures (1:1:1) of river sand, red too soil, and compost (mushroom rose bush or grass) plus size of plants (from 2 through to 8 cm) were examined in the greenhouse. In the laboratory both low and low and intermediate levels (64-205 mEs-¹ m-²) during micropropagation gave the best survival rates, around 42-48% on acclimatization where as higher levels (285-36 mEs-¹ m-²) were only 8-12* survived. Milled pine bark has been the best substrate so far, it had the best survival rate (50%) and plant growth. Surprisingly fertilizer did not improve the survival rate and as the trial continued to 8-12 weeks, it was eve-a detrimental to the plant survival and growth. They become fragile and more prone to insect attack, particulary during the critical hot and dry summer period. Plant size did not affect the survival rate, although the bigger ones had larger plants, more nodes and thicker stems. Considering these results we suggest low level of light (64 mEs-¹ m-²) during micropropagation, milled pine bark as a substrate for greenhouse trials, plants from the intermediate size (4 - 5 cm) and no use of fertilizer. MenosAbstract: In the semi-arid regions acclimatization process from tissue culture to the field is a problem compared to the humid tropics. The reasons are a long dry atmosphere and both diurnal and annual temperature range (from frost to 40°C). Previous data from trials of cassava (Manihot esculenta Crantz) in Mozambique, where IITA methods were compared to a locally adapted method, were not very successful, with 35% survial with adapted. IITA method. Studies to improve survial of ex vitro plantlets are still ongoing. Results are presented that include comparisons of light intensity levels in k-.he laboratory (64, 124, 205, 285, and 368 mEs-¹ m-²). Levels of fertilizer (NPK 94-253-51) (0,0.5,1,1-5 and 2.0g/
1), types of substrate, namely milled pine bark, vermiculite, and theree mixtures (1:1:1) of river sand, red too soil, and compost (mushroom rose bush or grass) plus size of plants (from 2 through to 8 cm) were examined in the greenhouse. In the laboratory both low and low and intermediate levels (64-205 mEs-¹ m-²) during micropropagation gave the best survival rates, around 42-48% on acclimatization where as higher levels (285-36 mEs-¹ m-²) were only 8-12* survived. Milled pine bark has been the best substrate so far, it had the best survival rate (50%) and plant growth. Surprisingly fertilizer did not improve the survival rate and as the trial continued to 8-12 weeks, it was eve-a detrimental to the plant survival and growth. They become fragile and more prone to inse... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02309naa a2200133 a 4500
001 1651878
005 2004-04-15
008 1998 bl uuuu u00u1 u #d
100 1 $aJORGE, M. A
245 $aFactors affecting the acclimatization of ex-vitro cassava.
260 $c1998
520 $aAbstract: In the semi-arid regions acclimatization process from tissue culture to the field is a problem compared to the humid tropics. The reasons are a long dry atmosphere and both diurnal and annual temperature range (from frost to 40°C). Previous data from trials of cassava (Manihot esculenta Crantz) in Mozambique, where IITA methods were compared to a locally adapted method, were not very successful, with 35% survial with adapted. IITA method. Studies to improve survial of ex vitro plantlets are still ongoing. Results are presented that include comparisons of light intensity levels in k-.he laboratory (64, 124, 205, 285, and 368 mEs-¹ m-²). Levels of fertilizer (NPK 94-253-51) (0,0.5,1,1-5 and 2.0g/ 1), types of substrate, namely milled pine bark, vermiculite, and theree mixtures (1:1:1) of river sand, red too soil, and compost (mushroom rose bush or grass) plus size of plants (from 2 through to 8 cm) were examined in the greenhouse. In the laboratory both low and low and intermediate levels (64-205 mEs-¹ m-²) during micropropagation gave the best survival rates, around 42-48% on acclimatization where as higher levels (285-36 mEs-¹ m-²) were only 8-12* survived. Milled pine bark has been the best substrate so far, it had the best survival rate (50%) and plant growth. Surprisingly fertilizer did not improve the survival rate and as the trial continued to 8-12 weeks, it was eve-a detrimental to the plant survival and growth. They become fragile and more prone to insect attack, particulary during the critical hot and dry summer period. Plant size did not affect the survival rate, although the bigger ones had larger plants, more nodes and thicker stems. Considering these results we suggest low level of light (64 mEs-¹ m-²) during micropropagation, milled pine bark as a substrate for greenhouse trials, plants from the intermediate size (4 - 5 cm) and no use of fertilizer.
700 1 $aROBERTS, A.
773 $tRevista Brasileira de Mandioca, Cruz das Almas$gv.17, p.53, nov., 1998. Suplemento.
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