| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
01/08/1992 |
|
Data da última atualização: |
08/04/2024 |
|
Autoria: |
JONES, R. C.; MARTIN, I. C. A. |
|
Título: |
Deep-freezing ram spermatozoa: the effects milk, yolk-citrate and synthetic diluents containing sugar. |
|
Ano de publicação: |
1965 |
|
Fonte/Imprenta: |
Journal of Reproduction and Fertility, v. 10, p. 413-423, Dec. 1965. |
|
DOI: |
10.1530/jrf.0.0100413 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract; In a factorial experiment skim milk, egg-yolk-citrate and synthetic diluents composed of fructose or lactose, sodium chloride and phosphate buffer containing 3·0% w/v of a lyophilized preparation of non-dialysable solids from (cow) milk were used as diluents for deepfreezing ram spermatozoa and for incubating spermatozoa at 37° C after thawing. All samples of semen were diluted forty-fold before freezing. Egg-yolk-citrate was inferior to skim milk as a diluent for freezing spermatozoa and for incubating spermatozoa after thawing. Of the two synthetic diluents, lactose synthetic was better for freezing spermatozoa whilst fructose synthetic was better for incubating spermatozoa after thawing. Only spermatozoa frozen in the lactose synthetic diluent and resuspended after thawing in the fructose synthetic survived incubation at 37° C for 2 hr as well as spermatozoa frozen and incubated in skim milk diluents. Two factorial experiments compared egg-yolk-citrate and skim milk as diluents for freezing semen diluted from ten- to forty-fold. At ten-fold dilution, revival was much the same after freezing in milk or egg-yolk-citrate. A twenty- or forty-fold dilution was better than a tenfold dilution in milk, but revival was depressed at these higher dilution rates after freezing in egg-yolk-citrate. When semen was frozen at a tenfold dilution it was advantageous to resuspend spermatozoa in a diluent free of glycerol after thawing. A diluent based on Krebs?Henseleit? Ringer solution containing 0·5% w/v of non-dialysable milk solids was better for incubating spermatozoa after thawing than egg-yolk-citrate or milk. A period of 5 hr equilibration at 5° C before freezing was better than 30 min equilibration. MenosAbstract; In a factorial experiment skim milk, egg-yolk-citrate and synthetic diluents composed of fructose or lactose, sodium chloride and phosphate buffer containing 3·0% w/v of a lyophilized preparation of non-dialysable solids from (cow) milk were used as diluents for deepfreezing ram spermatozoa and for incubating spermatozoa at 37° C after thawing. All samples of semen were diluted forty-fold before freezing. Egg-yolk-citrate was inferior to skim milk as a diluent for freezing spermatozoa and for incubating spermatozoa after thawing. Of the two synthetic diluents, lactose synthetic was better for freezing spermatozoa whilst fructose synthetic was better for incubating spermatozoa after thawing. Only spermatozoa frozen in the lactose synthetic diluent and resuspended after thawing in the fructose synthetic survived incubation at 37° C for 2 hr as well as spermatozoa frozen and incubated in skim milk diluents. Two factorial experiments compared egg-yolk-citrate and skim milk as diluents for freezing semen diluted from ten- to forty-fold. At ten-fold dilution, revival was much the same after freezing in milk or egg-yolk-citrate. A twenty- or forty-fold dilution was better than a tenfold dilution in milk, but revival was depressed at these higher dilution rates after freezing in egg-yolk-citrate. When semen was frozen at a tenfold dilution it was advantageous to resuspend spermatozoa in a diluent free of glycerol after thawing. A diluent based on Krebs?Henseleit? Ringer so... Mostrar Tudo |
|
Palavras-Chave: |
Cold Temperature; Diluente; In Vitro Techniques; Tecnologia do semen. |
|
Thesagro: |
Congelamento; Espermatozóide; Lactose; Ovino; Reprodução. |
|
Thesaurus Nal: |
Buffers; Egg yolk; Fructose; Pharmacology; Phosphates; Sheep; Sodium chloride; Spermatozoa. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02706naa a2200349 a 4500
001 1520498
005 2024-04-08
008 1965 bl uuuu u00u1 u #d
024 7 $a10.1530/jrf.0.0100413$2DOI
100 1 $aJONES, R. C.
245 $aDeep-freezing ram spermatozoa$bthe effects milk, yolk-citrate and synthetic diluents containing sugar.$h[electronic resource]
260 $c1965
520 $aAbstract; In a factorial experiment skim milk, egg-yolk-citrate and synthetic diluents composed of fructose or lactose, sodium chloride and phosphate buffer containing 3·0% w/v of a lyophilized preparation of non-dialysable solids from (cow) milk were used as diluents for deepfreezing ram spermatozoa and for incubating spermatozoa at 37° C after thawing. All samples of semen were diluted forty-fold before freezing. Egg-yolk-citrate was inferior to skim milk as a diluent for freezing spermatozoa and for incubating spermatozoa after thawing. Of the two synthetic diluents, lactose synthetic was better for freezing spermatozoa whilst fructose synthetic was better for incubating spermatozoa after thawing. Only spermatozoa frozen in the lactose synthetic diluent and resuspended after thawing in the fructose synthetic survived incubation at 37° C for 2 hr as well as spermatozoa frozen and incubated in skim milk diluents. Two factorial experiments compared egg-yolk-citrate and skim milk as diluents for freezing semen diluted from ten- to forty-fold. At ten-fold dilution, revival was much the same after freezing in milk or egg-yolk-citrate. A twenty- or forty-fold dilution was better than a tenfold dilution in milk, but revival was depressed at these higher dilution rates after freezing in egg-yolk-citrate. When semen was frozen at a tenfold dilution it was advantageous to resuspend spermatozoa in a diluent free of glycerol after thawing. A diluent based on Krebs?Henseleit? Ringer solution containing 0·5% w/v of non-dialysable milk solids was better for incubating spermatozoa after thawing than egg-yolk-citrate or milk. A period of 5 hr equilibration at 5° C before freezing was better than 30 min equilibration.
650 $aBuffers
650 $aEgg yolk
650 $aFructose
650 $aPharmacology
650 $aPhosphates
650 $aSheep
650 $aSodium chloride
650 $aSpermatozoa
650 $aCongelamento
650 $aEspermatozóide
650 $aLactose
650 $aOvino
650 $aReprodução
653 $aCold Temperature
653 $aDiluente
653 $aIn Vitro Techniques
653 $aTecnologia do semen
700 1 $aMARTIN, I. C. A.
773 $tJournal of Reproduction and Fertility$gv. 10, p. 413-423, Dec. 1965.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
21/12/2018 |
|
Data da última atualização: |
10/04/2024 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
PENNA, A. C. G.; OLIVEIRA, N. A. de; MENDONÇA, J. F. M. de; SIAS, G. R. F. V.; SOUZA, G. N. de. |
|
Afiliação: |
Anna Carolina Gonçalvez Penna, Universidade Presidente Antônio Carlos/Juiz de Fora; Naiara Aparecida de Oliveira, UFJF; Juliana França Monteiro de Mendonça, UFF; Gabriel Raposo Frauches Vieira Sias, UFJF; GUILHERME NUNES DE SOUZA, CNPGL. |
|
Título: |
Prevalência de Staphylococcus aureus e Streptococcus agalactiae em rebanhos leiteiros localizados na Zona da Mata de Minas Gerais. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 46, s55, 2018. Supl. 2. |
|
Idioma: |
Português |
|
Notas: |
Edição dos resumos do Encontro Nacional de Epidemiologia Veterinária, Porto Alegre, 2018. |
|
Palavras-Chave: |
Prevalência; Rebanhos bovinos leiteiros; Vacas infectadas. |
|
Categoria do assunto: |
H Saúde e Patologia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1102414/1/Prevalencia-de-Staphylococcus-aureus.pdf
|
|
Marc: |
LEADER 00757nam a2200193 a 4500
001 2102414
005 2024-04-10
008 2018 bl uuuu u00u1 u #d
100 1 $aPENNA, A. C. G.
245 $aPrevalência de Staphylococcus aureus e Streptococcus agalactiae em rebanhos leiteiros localizados na Zona da Mata de Minas Gerais.$h[electronic resource]
260 $aActa Scientiae Veterinariae, v. 46, s55, 2018. Supl. 2.$c2018
500 $aEdição dos resumos do Encontro Nacional de Epidemiologia Veterinária, Porto Alegre, 2018.
653 $aPrevalência
653 $aRebanhos bovinos leiteiros
653 $aVacas infectadas
700 1 $aOLIVEIRA, N. A. de
700 1 $aMENDONÇA, J. F. M. de
700 1 $aSIAS, G. R. F. V.
700 1 $aSOUZA, G. N. de
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
