Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
16/06/1997 |
Data da última atualização: |
17/05/2023 |
Autoria: |
HOSSAIN, M.; RAHMAN, S. M.; ISLAM, R.; JOARDER, O. I. |
Afiliação: |
Rajshahi University, Rajashahi, Bangladesh. |
Título: |
High efficiency plant regeneration from petiole explants of Carica papaya L. through organogenesis. |
Ano de publicação: |
1993 |
Fonte/Imprenta: |
Plant Cell Reports; n. 13, v.2, p. 99-102; 1993. |
Idioma: |
Inglês |
Conteúdo: |
Callus cultures were obrained from petiole explant of Carica papaya on MS medium containing 0.5-10.5 M naphthaleneatic acid (NAA) in combination with 0.5-5 M benzyladenine (BA). Hardgreen cali were transferred to MS medium containing 100 mgl-1 casein hydrolysate (CH) with specific BA-NAA formulation, where they developed adventition buds within 2 weeks of culture. Maximum number of adventitions buds were obtaned in 2 M BA and 0.1 M NAA. Shoot regeneration occurred from these adventitious buds by the end of the 4th weeks. Regenerated shoots were elongated in homone-free medium and rooted in half-strength MS fortified with 3 M NAA and 0.5 M gibberelic acid (GA3). The regenants were transferred to soil after acclimatization. |
Thesagro: |
Mamão. |
Categoria do assunto: |
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Marc: |
LEADER 01211naa a2200169 a 4500 001 1647923 005 2023-05-17 008 1993 bl uuuu u00u1 u #d 100 1 $aHOSSAIN, M. 245 $aHigh efficiency plant regeneration from petiole explants of Carica papaya L. through organogenesis.$h[electronic resource] 260 $c1993 520 $aCallus cultures were obrained from petiole explant of Carica papaya on MS medium containing 0.5-10.5 M naphthaleneatic acid (NAA) in combination with 0.5-5 M benzyladenine (BA). Hardgreen cali were transferred to MS medium containing 100 mgl-1 casein hydrolysate (CH) with specific BA-NAA formulation, where they developed adventition buds within 2 weeks of culture. Maximum number of adventitions buds were obtaned in 2 M BA and 0.1 M NAA. Shoot regeneration occurred from these adventitious buds by the end of the 4th weeks. Regenerated shoots were elongated in homone-free medium and rooted in half-strength MS fortified with 3 M NAA and 0.5 M gibberelic acid (GA3). The regenants were transferred to soil after acclimatization. 650 $aMamão 700 1 $aRAHMAN, S. M. 700 1 $aISLAM, R. 700 1 $aJOARDER, O. I. 773 $tPlant Cell Reports;$gn. 13
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |