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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
26/12/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ASCARI, I. J.; MARTINS, S. C.; CAMARGO, L. S. de A.; MENDEZ-OTERO, R.; JASMIN. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Development of bovine embryos in vitro in coculture with murine mesenchymal stem cells and embryonic fibroblasts. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 45, n. 6, p. 1827-1837, 2018. |
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DOI: |
10.1007/s11033-018-4329-y |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Despite the progress on development of new culture media, in vitro-produced embryos still display lower quality when compared to the in vivo-produced counterparts. Coculture has been reconsidered as an alternative to improve embryo quality. Mesenchymal stem cells (MSC) and murine embryonic fibroblasts (MEF) have been extensively used as feeder layers due to their capacity to release growth factors. In the present study we investigated the effect of these feeder layers in oocyte maturation and/or embryo development under in vitro conditions. Oocytes were matured in control (CTRL) conditions or in coculture with MSC or MEF. In vitro fertilization and embryo culture until fourth day were performed in CTRL condition for all groups. Embryos from fourth day on were then cultured until the eighth day in CTRL or in coculture system. No significant differences for metaphase II stage and apoptosis in oocytes were found among the groups. There was also no difference among the groups when we evaluated blastocyst formation on the seventh and eighth day, with exception of a higher hatched blastocyst rate in the group maturated and cultivated in CTRL condition when compared to the group matured and cocultured with MSC. Also no difference was observed in the number of cells in the whole embryos, in the inner cell mass, in the trophoblast and at apoptotic stage on the eighth day. We conclude that coculture with MSC or MEF during maturation and/or embryo development do not enhance the in vitro production of bovine embryos. MenosAbstract Despite the progress on development of new culture media, in vitro-produced embryos still display lower quality when compared to the in vivo-produced counterparts. Coculture has been reconsidered as an alternative to improve embryo quality. Mesenchymal stem cells (MSC) and murine embryonic fibroblasts (MEF) have been extensively used as feeder layers due to their capacity to release growth factors. In the present study we investigated the effect of these feeder layers in oocyte maturation and/or embryo development under in vitro conditions. Oocytes were matured in control (CTRL) conditions or in coculture with MSC or MEF. In vitro fertilization and embryo culture until fourth day were performed in CTRL condition for all groups. Embryos from fourth day on were then cultured until the eighth day in CTRL or in coculture system. No significant differences for metaphase II stage and apoptosis in oocytes were found among the groups. There was also no difference among the groups when we evaluated blastocyst formation on the seventh and eighth day, with exception of a higher hatched blastocyst rate in the group maturated and cultivated in CTRL condition when compared to the group matured and cocultured with MSC. Also no difference was observed in the number of cells in the whole embryos, in the inner cell mass, in the trophoblast and at apoptotic stage on the eighth day. We conclude that coculture with MSC or MEF during maturation and/or embryo development do not enhance th... Mostrar Tudo |
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Palavras-Chave: |
Bovine; In vitro embryonic development; Mesenchymal stem cells; Murine embryonic fibroblast. |
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Thesaurus Nal: |
Coculture. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02308naa a2200241 a 4500
001 2102511
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1007/s11033-018-4329-y$2DOI
100 1 $aASCARI, I. J.
245 $aDevelopment of bovine embryos in vitro in coculture with murine mesenchymal stem cells and embryonic fibroblasts.$h[electronic resource]
260 $c2018
520 $aAbstract Despite the progress on development of new culture media, in vitro-produced embryos still display lower quality when compared to the in vivo-produced counterparts. Coculture has been reconsidered as an alternative to improve embryo quality. Mesenchymal stem cells (MSC) and murine embryonic fibroblasts (MEF) have been extensively used as feeder layers due to their capacity to release growth factors. In the present study we investigated the effect of these feeder layers in oocyte maturation and/or embryo development under in vitro conditions. Oocytes were matured in control (CTRL) conditions or in coculture with MSC or MEF. In vitro fertilization and embryo culture until fourth day were performed in CTRL condition for all groups. Embryos from fourth day on were then cultured until the eighth day in CTRL or in coculture system. No significant differences for metaphase II stage and apoptosis in oocytes were found among the groups. There was also no difference among the groups when we evaluated blastocyst formation on the seventh and eighth day, with exception of a higher hatched blastocyst rate in the group maturated and cultivated in CTRL condition when compared to the group matured and cocultured with MSC. Also no difference was observed in the number of cells in the whole embryos, in the inner cell mass, in the trophoblast and at apoptotic stage on the eighth day. We conclude that coculture with MSC or MEF during maturation and/or embryo development do not enhance the in vitro production of bovine embryos.
650 $aCoculture
653 $aBovine
653 $aIn vitro embryonic development
653 $aMesenchymal stem cells
653 $aMurine embryonic fibroblast
700 1 $aMARTINS, S. C.
700 1 $aCAMARGO, L. S. de A.
700 1 $aMENDEZ-OTERO, R.
700 1 $aJASMIN
773 $tMolecular Biology Reports$gv. 45, n. 6, p. 1827-1837, 2018.
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Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
02/07/2020 |
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Data da última atualização: |
10/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
RITTL, T. F.; CANISARES, L.; SAGRILO, E.; BAHL, K. B.; DANNENMANN, M.; CERRI, C. E. P. |
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Afiliação: |
Tatiana F. Rittl, USP-ESALQ, Piracicaba, SP.; Luiza Canisares, USP-ESALQ, Piracicaba, SP.; EDVALDO SAGRILO, CPAMN; Klaus Butterbach Bahl, Karlsruhe Institute of Technology, Institute of Meteorology and Climate Research, Atmospheric Environmental Research (IMK-IFU), Garmisch-Parten-kirchen 82467 (Germany); Michael Dannenmann, Karlsruhe Institute of Technology, Institute of Meteorology and Climate Research, Atmospheric Environmental Research (IMK-IFU), Garmisch-Parten-kirchen 82467 (Germany); Carlos E. P. Cerri, USP-ESALQ, Piracicaba, SP. |
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Título: |
Temperature sensitivity of soil organic matter decomposition varies with biocharapplication and soil type. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Pedosphere, v. 30, n. 3, p. 336-342, 2020. |
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ISSN: |
1002-0160 |
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DOI: |
10.1016/S1002-0160(20)60013-3 |
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Idioma: |
Inglês |
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Conteúdo: |
Biochar application has the potential to improve soil fertility and increase soil carbon stock, especially in tropical regions. Information on the temperaturesensitivity of carbon dioxide (CO2) evolution from biochar-amended soils at very high temperatures, as observed for tropical surface soils, is limited buturgently needed for the development of region-specific biochar management targeted to optimize biochar effects on soil functions. Here, we investigated thetemperature sensitivity of soil respiration to the addition of different rates ofMiscanthusbiochar (0, 6.25, 12.5, and 25 Mg ha−1) in two types of soils withcontrasting textures. Biochar-amended soil treatments and their controls were incubated at constant temperatures of 20, 30, and 40◦C. Overall, our resultsshow that: i) considering data from all treatments and temperatures, the addition of biochar decreased soil CO2emissions when compared to untreated soils;ii) CO2emissions from biochar-amended soils had a higher temperature sensitivity than those from biochar-free soils; iii) the temperature sensitivity of soilrespiration in sandy soils was higher than that in clay soils; and iv) for clay soils, relative increases in soil CO2emissions from biochar-amended soils werehigher when the temperature increased from 30 to 40◦C, while for sandy soils, the highest temperature responses of soil respiration were observed whenincreasing the temperature from 20 to 30◦C. Together, these findings suggest a significantly reduced potential to increase soil organic carbon stocks whenMiscanthusbiochar is applied to tropical soils at high surface temperatures, which could be counteracted by the soil- and weather-specific timing of biocharapplication. MenosBiochar application has the potential to improve soil fertility and increase soil carbon stock, especially in tropical regions. Information on the temperaturesensitivity of carbon dioxide (CO2) evolution from biochar-amended soils at very high temperatures, as observed for tropical surface soils, is limited buturgently needed for the development of region-specific biochar management targeted to optimize biochar effects on soil functions. Here, we investigated thetemperature sensitivity of soil respiration to the addition of different rates ofMiscanthusbiochar (0, 6.25, 12.5, and 25 Mg ha−1) in two types of soils withcontrasting textures. Biochar-amended soil treatments and their controls were incubated at constant temperatures of 20, 30, and 40◦C. Overall, our resultsshow that: i) considering data from all treatments and temperatures, the addition of biochar decreased soil CO2emissions when compared to untreated soils;ii) CO2emissions from biochar-amended soils had a higher temperature sensitivity than those from biochar-free soils; iii) the temperature sensitivity of soilrespiration in sandy soils was higher than that in clay soils; and iv) for clay soils, relative increases in soil CO2emissions from biochar-amended soils werehigher when the temperature increased from 30 to 40◦C, while for sandy soils, the highest temperature responses of soil respiration were observed whenincreasing the temperature from 20 to 30◦C. Together, these findings suggest a... Mostrar Tudo |
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Thesagro: |
Dióxido de Carbono; Efeito Estufa; Solo Arenoso; Solo Argiloso; Solo Tropical. |
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Thesaurus NAL: |
Carbon dioxide; Clay soils; Greenhouse gas emissions; Miscanthus; Sandy soils; Tropical soils. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02703naa a2200337 a 4500
001 2123590
005 2020-12-10
008 2020 bl uuuu u00u1 u #d
022 $a1002-0160
024 7 $a10.1016/S1002-0160(20)60013-3$2DOI
100 1 $aRITTL, T. F.
245 $aTemperature sensitivity of soil organic matter decomposition varies with biocharapplication and soil type.$h[electronic resource]
260 $c2020
520 $aBiochar application has the potential to improve soil fertility and increase soil carbon stock, especially in tropical regions. Information on the temperaturesensitivity of carbon dioxide (CO2) evolution from biochar-amended soils at very high temperatures, as observed for tropical surface soils, is limited buturgently needed for the development of region-specific biochar management targeted to optimize biochar effects on soil functions. Here, we investigated thetemperature sensitivity of soil respiration to the addition of different rates ofMiscanthusbiochar (0, 6.25, 12.5, and 25 Mg ha−1) in two types of soils withcontrasting textures. Biochar-amended soil treatments and their controls were incubated at constant temperatures of 20, 30, and 40◦C. Overall, our resultsshow that: i) considering data from all treatments and temperatures, the addition of biochar decreased soil CO2emissions when compared to untreated soils;ii) CO2emissions from biochar-amended soils had a higher temperature sensitivity than those from biochar-free soils; iii) the temperature sensitivity of soilrespiration in sandy soils was higher than that in clay soils; and iv) for clay soils, relative increases in soil CO2emissions from biochar-amended soils werehigher when the temperature increased from 30 to 40◦C, while for sandy soils, the highest temperature responses of soil respiration were observed whenincreasing the temperature from 20 to 30◦C. Together, these findings suggest a significantly reduced potential to increase soil organic carbon stocks whenMiscanthusbiochar is applied to tropical soils at high surface temperatures, which could be counteracted by the soil- and weather-specific timing of biocharapplication.
650 $aCarbon dioxide
650 $aClay soils
650 $aGreenhouse gas emissions
650 $aMiscanthus
650 $aSandy soils
650 $aTropical soils
650 $aDióxido de Carbono
650 $aEfeito Estufa
650 $aSolo Arenoso
650 $aSolo Argiloso
650 $aSolo Tropical
700 1 $aCANISARES, L.
700 1 $aSAGRILO, E.
700 1 $aBAHL, K. B.
700 1 $aDANNENMANN, M.
700 1 $aCERRI, C. E. P.
773 $tPedosphere$gv. 30, n. 3, p. 336-342, 2020.
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