|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
30/07/2020 |
Data da última atualização: |
25/08/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
IWANICKI, N. S.; MASCARIN, G. M.; MORENO, S. G.; EILENBERG, J.; DELALIBERA JUNIOR, I. |
Afiliação: |
NATASHA SANT'ANNA IWANICKI, ESALQ-USP; GABRIEL MOURA MASCARIN, CNPMA; SARA GIRO MORENO, ESALQ-USP; JORGEN EILENBERG, University of Copenhagen; ITALO DELALIBERA JUNIOR, ESALQ-USP. |
Título: |
Growth kinetic and nitrogen source optimization for liquid culture fermentation of Metarhizium robertsii blastospores and bioefficacy against the corn leafhopper Dalbulus maidis. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, v. 36, article 71, 2020. |
ISSN: |
1573-0972 |
DOI: |
https://doi.org/10.1007/s11274-020-02844-z |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The cosmopolitan entomopathogenic and root endophytic fungus Metarhizium robertsii has a versatile lifestyle and during liquid fermentation undergoes a dimorphic transformation from hyphae to conidia or microsclerotia, or from hyphae to blastospores. In all cases, these processes are mediated by environmental and nutritional cues. Blastospores could be used in spray applications to control arthropod pests above ground and may serve as an attractive alternative to the traditional solid-grown aerial conidial spores of Metarhizium spp. found in commercial products. Nitrogen is a vital nutrient in cell metabolism and growth; however, it is the expensive component in liquid cultures of entomopathogenic fungi. Our goals in this study were to optimize nitrogen sources and titers for maximum production of M. robertsii blastospores cultured in shake flasks at highly aerated conditions and to further determine their virulence against the corn leafhopper Dalbulus maidis, an important vector of serious pathogens in maize crops worldwide. Our fermentation studies revealed that the low-cost corn steep liquor (CSL) was the most suitable nitrogen source to improve blastospore growth in M. robertsii.The growth kinetic assays determined the optimal titer of 80 g L-1 and a yield up to 4.7 × 100.000.000 cells mL-1 within 5 days of cultivation (3 days preculture and 2 days culture), at a total cost of US$0.30 L-1. Moreover, the blastospore growth kinetic was strongly dependent on glucose and nitrogen consumptions accompanied by a slight drop in the culture pH. Insect bioassays evidenced a high virulence of these blastospores, either as dried or fresh cells, to D. maidis adults fed on maize plants. Our findings provide insights into the nutritional requirements for optimal and cost-efficient production of M. robertsii blastospores and elucidate the potential of blastospores as an ecofriendly tool against the corn leafhopper. MenosAbstract: The cosmopolitan entomopathogenic and root endophytic fungus Metarhizium robertsii has a versatile lifestyle and during liquid fermentation undergoes a dimorphic transformation from hyphae to conidia or microsclerotia, or from hyphae to blastospores. In all cases, these processes are mediated by environmental and nutritional cues. Blastospores could be used in spray applications to control arthropod pests above ground and may serve as an attractive alternative to the traditional solid-grown aerial conidial spores of Metarhizium spp. found in commercial products. Nitrogen is a vital nutrient in cell metabolism and growth; however, it is the expensive component in liquid cultures of entomopathogenic fungi. Our goals in this study were to optimize nitrogen sources and titers for maximum production of M. robertsii blastospores cultured in shake flasks at highly aerated conditions and to further determine their virulence against the corn leafhopper Dalbulus maidis, an important vector of serious pathogens in maize crops worldwide. Our fermentation studies revealed that the low-cost corn steep liquor (CSL) was the most suitable nitrogen source to improve blastospore growth in M. robertsii.The growth kinetic assays determined the optimal titer of 80 g L-1 and a yield up to 4.7 × 100.000.000 cells mL-1 within 5 days of cultivation (3 days preculture and 2 days culture), at a total cost of US$0.30 L-1. Moreover, the blastospore growth kinetic was strongly dependent on gluco... Mostrar Tudo |
Palavras-Chave: |
Biocontrol; Dimorphic growth; Fungal entomopathogen; Fungal nutrition. |
Thesagro: |
Fungo Para Controle Biológico; Milho; Praga de Planta; Zea Mays. |
Thesaurus Nal: |
Biological control agents; Corn; Entomopathogenic fungi; Metarhizium robertsii; Plant pests; Virulence. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 03118naa a2200361 a 4500 001 2124093 005 2021-08-25 008 2020 bl uuuu u00u1 u #d 022 $a1573-0972 024 7 $ahttps://doi.org/10.1007/s11274-020-02844-z$2DOI 100 1 $aIWANICKI, N. S. 245 $aGrowth kinetic and nitrogen source optimization for liquid culture fermentation of Metarhizium robertsii blastospores and bioefficacy against the corn leafhopper Dalbulus maidis.$h[electronic resource] 260 $c2020 520 $aAbstract: The cosmopolitan entomopathogenic and root endophytic fungus Metarhizium robertsii has a versatile lifestyle and during liquid fermentation undergoes a dimorphic transformation from hyphae to conidia or microsclerotia, or from hyphae to blastospores. In all cases, these processes are mediated by environmental and nutritional cues. Blastospores could be used in spray applications to control arthropod pests above ground and may serve as an attractive alternative to the traditional solid-grown aerial conidial spores of Metarhizium spp. found in commercial products. Nitrogen is a vital nutrient in cell metabolism and growth; however, it is the expensive component in liquid cultures of entomopathogenic fungi. Our goals in this study were to optimize nitrogen sources and titers for maximum production of M. robertsii blastospores cultured in shake flasks at highly aerated conditions and to further determine their virulence against the corn leafhopper Dalbulus maidis, an important vector of serious pathogens in maize crops worldwide. Our fermentation studies revealed that the low-cost corn steep liquor (CSL) was the most suitable nitrogen source to improve blastospore growth in M. robertsii.The growth kinetic assays determined the optimal titer of 80 g L-1 and a yield up to 4.7 × 100.000.000 cells mL-1 within 5 days of cultivation (3 days preculture and 2 days culture), at a total cost of US$0.30 L-1. Moreover, the blastospore growth kinetic was strongly dependent on glucose and nitrogen consumptions accompanied by a slight drop in the culture pH. Insect bioassays evidenced a high virulence of these blastospores, either as dried or fresh cells, to D. maidis adults fed on maize plants. Our findings provide insights into the nutritional requirements for optimal and cost-efficient production of M. robertsii blastospores and elucidate the potential of blastospores as an ecofriendly tool against the corn leafhopper. 650 $aBiological control agents 650 $aCorn 650 $aEntomopathogenic fungi 650 $aMetarhizium robertsii 650 $aPlant pests 650 $aVirulence 650 $aFungo Para Controle Biológico 650 $aMilho 650 $aPraga de Planta 650 $aZea Mays 653 $aBiocontrol 653 $aDimorphic growth 653 $aFungal entomopathogen 653 $aFungal nutrition 700 1 $aMASCARIN, G. M. 700 1 $aMORENO, S. G. 700 1 $aEILENBERG, J. 700 1 $aDELALIBERA JUNIOR, I. 773 $tWorld Journal of Microbiology and Biotechnology$gv. 36, article 71, 2020.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Meio Ambiente (CNPMA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
12/12/2018 |
Data da última atualização: |
18/12/2018 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
GONÇALVES, A. A.; MARIN, D. F. D.; SILVA, T. V. G.; LIMA, A. J. M. de; MORAIS, M. A. R.; SOUZA, E. B. de; MELO, D. N. de; ROLIM FILHO, S. T.; GARCIA, A. R.; OHASHI, O. M. |
Afiliação: |
Arnaldo Algaranhar Gonçalves, UFPA; Diego Fernando Dubeibe Marin, UFPA; Tiago Velasco Guimarães Silva, UFPA; Ana Júlia Mota de Lima, UFPA; Mauro Andrey Rodrigues Morais, UFPA; Eduardo Baia de Souza, UFPA; Dayana Neves de Melo, Universidade Federal Rural da Amazônia; Sebastião Tavares Rolim Filho, Universidade Federal Rural da Amazônia; ALEXANDRE ROSSETTO GARCIA, CPPSE; Otávio Mitio Ohashi, Universidade Federal Rural da Amazônia. |
Título: |
Anormalidades morfológicas e danos no DNA espermático em touros bubalinos (Bubalus bubalis) submetidos à insulação testicular. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
In: REUNIÃO ANUAL DA ABRAA, 3., 2018, Campo Grande, MS. Anais... Campo Grande, MS: UFMS, 2018. |
Páginas: |
p.168-171. |
Idioma: |
Português |
Notas: |
8 a 9 de junho de 2018. |
Conteúdo: |
e aim was to understand the performance of testicular thermal stress on the morphology, compaction and integrity of DNA in the different phases of the spermatogenic cycle in buffaloes, using chromomycin A3 and acridine orange respectively. Five adult bulls undergoing testicular insulation for 48 hours and semen collected once every seven days, divided into pre and post testicular insulation. e post-insulation testicular temperature increased 3.2ºC (p<0.05) in relation to the control. ere was an increase (p<0.05) in the deprotamination of the spermatic chromatin in the seventh week a#er insulation compared to the control (11.0 ±3.1 and 3.0 ±2.8), but there was no difference in DNA fragmentation a#er insulation. However, head defects showed an increase (p<0.05) in the seventh week in relation to the control (0.7 ±0.2 and 0.13 ±0.16). erefore, the increase in testicular temperature in buffaloes had a negative influence on spermatogonia and primary spermatocytes on chromatin protamination. |
Palavras-Chave: |
Cromatina; Fragmentação; Protamina. |
Thesagro: |
Bubalus Bubalis. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/188213/1/AnormalidadesMorfologicasDNA.pdf
|
Marc: |
LEADER 01937nam a2200289 a 4500 001 2101231 005 2018-12-18 008 2018 bl uuuu u00u1 u #d 100 1 $aGONÇALVES, A. A. 245 $aAnormalidades morfológicas e danos no DNA espermático em touros bubalinos (Bubalus bubalis) submetidos à insulação testicular.$h[electronic resource] 260 $aIn: REUNIÃO ANUAL DA ABRAA, 3., 2018, Campo Grande, MS. Anais... Campo Grande, MS: UFMS$c2018 300 $ap.168-171. 500 $a8 a 9 de junho de 2018. 520 $ae aim was to understand the performance of testicular thermal stress on the morphology, compaction and integrity of DNA in the different phases of the spermatogenic cycle in buffaloes, using chromomycin A3 and acridine orange respectively. Five adult bulls undergoing testicular insulation for 48 hours and semen collected once every seven days, divided into pre and post testicular insulation. e post-insulation testicular temperature increased 3.2ºC (p<0.05) in relation to the control. ere was an increase (p<0.05) in the deprotamination of the spermatic chromatin in the seventh week a#er insulation compared to the control (11.0 ±3.1 and 3.0 ±2.8), but there was no difference in DNA fragmentation a#er insulation. However, head defects showed an increase (p<0.05) in the seventh week in relation to the control (0.7 ±0.2 and 0.13 ±0.16). erefore, the increase in testicular temperature in buffaloes had a negative influence on spermatogonia and primary spermatocytes on chromatin protamination. 650 $aBubalus Bubalis 653 $aCromatina 653 $aFragmentação 653 $aProtamina 700 1 $aMARIN, D. F. D. 700 1 $aSILVA, T. V. G. 700 1 $aLIMA, A. J. M. de 700 1 $aMORAIS, M. A. R. 700 1 $aSOUZA, E. B. de 700 1 $aMELO, D. N. de 700 1 $aROLIM FILHO, S. T. 700 1 $aGARCIA, A. R. 700 1 $aOHASHI, O. M.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|