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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
03/10/2018 |
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Data da última atualização: |
03/10/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LIMA, A. M.; CUNHA, E. F. M.; ISHIDA, A. K. N.; PEREIRA, A. C. da C.; REIS, S. P. dos; SOUZA, C. R. B. de. |
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Afiliação: |
Aline Medeiros Lima, UFRA/UFPA; ELISA FERREIRA MOURA CUNHA, CPATU; ALESSANDRA KEIKO NAKASONE ISHIDA, CPATU; Angelo Cleiton da Costa Pereira, UFRA/UFPA; Sávio Pinho dos Reis, UFPA/UEPA; Cláudia Regina Batista de Souza, UFPA. |
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Título: |
Expression profiles of defense genes in cassava storage roots upon exposure to Phytopythium sp., causal agent of soft root rot disease. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Physiological and Molecular Plant Pathology, v. 104, p. 23-30, Dec. 2018. |
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DOI: |
10.1016/j.pmpp.2018.09.001 |
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Idioma: |
Inglês |
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Conteúdo: |
Cassava is one of the most important crops in Africa, Asia and Latin America, where millions of people depend on its starchy storage roots as their main source of energy. In Brazil, cassava is cultivated in all regions; however, soft root rot disease has hampered its production mainly in the Amazon region. The identification of Phytopythium sp., as the causal agent of soft root rot disease in cassava plants collected in Brazil, was recently reported. Here, our main aim was to evaluate changes in expression levels of genes possibly involved in compatible cassava- Phytopythium sp. interaction. Semi-quantitative RT-PCR assays were performed to evaluate the expression levels of genes coding for proteins with known functions in plant defense, such as enzymes involved in oxidative burst and the phenylpropanoid pathway, in detached cassava storage roots inoculated and noninoculated with pathogen. Our results showed that cassava responded to pathogen infection through up-regulation of genes coding for superoxide dismutase, phenylalanine ammonia-lyase, chalcone synthase, cysteine proteinase inhibitor and RING zinc finger protein. Furthermore, the expression pattern of the allergenic-related glutamic acid-rich protein and translationally controlled tumor protein genes in detached roots used here validated the absence of post-harvest physiological deterioration, a known endogenous disorder affecting the storage roots of cassava at 24?72 h after harvest. This is the first study of interaction between cassava and Phytopythium sp. at the molecular level contributing to understanding how cassava responds to pathogen infection, as well as to future strategies of molecular breeding regarding the tolerance to soft root rot disease. MenosCassava is one of the most important crops in Africa, Asia and Latin America, where millions of people depend on its starchy storage roots as their main source of energy. In Brazil, cassava is cultivated in all regions; however, soft root rot disease has hampered its production mainly in the Amazon region. The identification of Phytopythium sp., as the causal agent of soft root rot disease in cassava plants collected in Brazil, was recently reported. Here, our main aim was to evaluate changes in expression levels of genes possibly involved in compatible cassava- Phytopythium sp. interaction. Semi-quantitative RT-PCR assays were performed to evaluate the expression levels of genes coding for proteins with known functions in plant defense, such as enzymes involved in oxidative burst and the phenylpropanoid pathway, in detached cassava storage roots inoculated and noninoculated with pathogen. Our results showed that cassava responded to pathogen infection through up-regulation of genes coding for superoxide dismutase, phenylalanine ammonia-lyase, chalcone synthase, cysteine proteinase inhibitor and RING zinc finger protein. Furthermore, the expression pattern of the allergenic-related glutamic acid-rich protein and translationally controlled tumor protein genes in detached roots used here validated the absence of post-harvest physiological deterioration, a known endogenous disorder affecting the storage roots of cassava at 24?72 h after harvest. This is the first study of inter... Mostrar Tudo |
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Thesagro: |
Defesa Vegetal; Doença; Doença de Planta; Mandioca; Podridão Radicular. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02563naa a2200253 a 4500
001 2096813
005 2018-10-03
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.pmpp.2018.09.001$2DOI
100 1 $aLIMA, A. M.
245 $aExpression profiles of defense genes in cassava storage roots upon exposure to Phytopythium sp., causal agent of soft root rot disease.$h[electronic resource]
260 $c2018
520 $aCassava is one of the most important crops in Africa, Asia and Latin America, where millions of people depend on its starchy storage roots as their main source of energy. In Brazil, cassava is cultivated in all regions; however, soft root rot disease has hampered its production mainly in the Amazon region. The identification of Phytopythium sp., as the causal agent of soft root rot disease in cassava plants collected in Brazil, was recently reported. Here, our main aim was to evaluate changes in expression levels of genes possibly involved in compatible cassava- Phytopythium sp. interaction. Semi-quantitative RT-PCR assays were performed to evaluate the expression levels of genes coding for proteins with known functions in plant defense, such as enzymes involved in oxidative burst and the phenylpropanoid pathway, in detached cassava storage roots inoculated and noninoculated with pathogen. Our results showed that cassava responded to pathogen infection through up-regulation of genes coding for superoxide dismutase, phenylalanine ammonia-lyase, chalcone synthase, cysteine proteinase inhibitor and RING zinc finger protein. Furthermore, the expression pattern of the allergenic-related glutamic acid-rich protein and translationally controlled tumor protein genes in detached roots used here validated the absence of post-harvest physiological deterioration, a known endogenous disorder affecting the storage roots of cassava at 24?72 h after harvest. This is the first study of interaction between cassava and Phytopythium sp. at the molecular level contributing to understanding how cassava responds to pathogen infection, as well as to future strategies of molecular breeding regarding the tolerance to soft root rot disease.
650 $aDefesa Vegetal
650 $aDoença
650 $aDoença de Planta
650 $aMandioca
650 $aPodridão Radicular
700 1 $aCUNHA, E. F. M.
700 1 $aISHIDA, A. K. N.
700 1 $aPEREIRA, A. C. da C.
700 1 $aREIS, S. P. dos
700 1 $aSOUZA, C. R. B. de
773 $tPhysiological and Molecular Plant Pathology$gv. 104, p. 23-30, Dec. 2018.
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Embrapa Amazônia Oriental (CPATU) |
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| 4. |  | OLIVEIRA, L. C.; ISHIDA, A. K. N.; SILVA, C. B. T.; FERREIRA, F. C. Atividade antifúngica de extratos de Morinda citrifolia E Piper nigrum sobre Rhizoctonia solani. Tropical Plant Pathology, Brasília, DF, v. 37, ago. 2012. Suplemento, ref. 465. Edição dos Resumos do 45º Congresso Brasileiro de Fitopatologia, Manaus, 2012. CBFito 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 5. | | FERREIRA, S. da C.; ISHIDA, A. K. N.; SOUZA FILHO, A. P. da S.; TOLENTINO, C. Atividades antibacteriana de bergenina sobre xanthomonas axonopodis pv. passiflorae. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA, 16., 2012, Belém, PA. Anais... Belém, PA: Embrapa Amazônia Oriental, 2012. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 6. | | SOUZA, R. M.; ZACARONI, A. B.; ISHIDA, A. K. N.; RESENDE, M. L. V. Acibenzolar-S-metil (ASM), silicatos, fosfitos e Ecolife® no controle da mancha angular do algodoeiro em casa-de-vegetação. Tropical Plant Pathology, v. 34, Supl., p. S15, ago. 2009. Resumos do 42º Congresso Brasileiro de Fitopatologia.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 7. | | PIRES, H. C. G.; LAMEIRA, O. A.; ISHIDA, A. K. N.; CARVALHO, A. N. C. de. Ação da óleoresina de copaíba na inibição do crescimento micelial in vitro de fitopatógenos. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 21., 2017, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental, 2017.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 9. | | BEZERRA, V. F. G.; ISHIDA, A. K. N.; CARDOSO, S. V. D.; OLIVEIRA, L. C. de. Caracterização de isolados de Xanthomonas axonopodis pv. manihotis. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA ORIENTAL, 21., 2017, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental, 2017.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 10. | | SILVA, R. T. da; ISHIDA, A. K. N.; ALMEIDA, C. A.; CARDOSO, S, V. D. Efeito da temperatura no crescimento de Xanthomonas axonopodis pv. passiflorae e Xanthomonas axonopodis pv. manihotis. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA, 18.; SEMINÁRIO DE PÓS-GRADUAÇÃO DA EMBRAPA AMAZÔNIA ORIENTAL, 2., 2014, Belém, PA. Anais. Belém, PA: Embrapa Amazônia Oriental, 2014. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 12. | | ZACARONI, A. B.; SOUZA, R. M.; ISHIDA, A. K. N.; RESENDE, M. L. V. Efeito do acibenzolar-S-metil (ASM), extrato de folhas de graviola, extratos de folha de goiaba, Niphokam® e Agro-Mos® no controle da mancha angular do algodoeiro em casa-de-vegetação. Tropical Plant Pathology, v. 34, Supl., p. S8, ago. 2009. Resumos do 42º Congresso Brasileiro de Fitopatologia.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 139 | |
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