Data/hora: 01/06/2024 16:42:37

Biblioteca(s): 	Embrapa Caprinos e Ovinos.	
Data corrente: 	02/05/1996	
Data da última atualização: 	07/03/2022	
Autoria: 	KATO, S.; IRITANI, A.; NISHIKAWA, Y.	
Título: 	Effect of low levels of carbon dioxide on aerobic metabolism and motility of goat spermatozoa.	
Ano de publicação: 	1978	
Fonte/Imprenta: 	The Japanese Journal of Zootechnical Science, v. 49, n. 1, p. 16-22, Jan. 1978.	
Idioma: 	Japonês	
Conteúdo: 	Abstract: The effect of low levels of CO/sub 2/ on the aerobic metabolism and motility of washed goat spermatozoa was investigated in culture media at pH 7.0 and in culture media containing 25 or 50 mM bicarbonate. The results obtained are as follows. 1) Both aerobic glycolysis and O/sub 2/ uptake by spermatozoa were stimulated in the presence of metabolic CO/sub 2/ as compared with the media without CO/sub 2/ at pH 7.0. The initial level of CO/sub 2/ in the gas phase was approximately 0.3% in a flask incubated with metabolic CO/sub 2/. However, the stimulatory effect of CO/sub 2/ was not enhanced even when the CO/sub 2/ level in the gas phase was increased from 0.3 to 1.0% or from 1 to 3%; this suggests that only a small amount of CO/sub 2/ is required for the maximum aerobic metabolism of goat spermatozoa at pH 7.0. Low levels of CO/sub 2/ stimulated glycolysis and O/sub 2/ uptake to almost the same extent. Although O/sub 2/ uptake with lactate as substrate was greater than that with glucose, the extent of stimulation of O/sub 2/ uptake by CO/sub 2/ did not differ between these two substrates. There was no difference in the maintenance of motility between spermatozoa incubated in media with or without low levels of CO/sub 2/. 2) In the presence of 25 mM bicarbonate in the media, glycolysis, O/sub 2/ uptake and sperm motility were markedly inhibited at 1% CO/sub 2/ as compared to those at 2 and 3% CO/sub 2/. Glycolysis and /sup 14/CO/sub 2/ production from glucose-U-/sup 14/C were most active at either 2.5 or 5.0%CO/sub 2/ and good motility was maintained during incubation periods at CO/sub 2/ levels above 2.5%. On the other hand, the optimal level of CO/sub 2/ for glycolysis and /sup 14/CO/sub 2/ production was around 5.0 to 7.5% in the presence of 50 mM bicarbonate; more than 5.0% of CO/sub 2/ was required for the maintenance of sperm motility.	
Palavras-Chave: 	Acid carbonates; Andrologia; In vitro; Living orgs.	
Thesagro: 	Caprino; Espermatozóide; Reprodução animal; Sêmen.	
Thesaurus Nal: 	Aldehydes; Carbon dioxide; Goats; Heterocyclic oxygen compounds; Inorganic acids; Lactic acid; Mortality; Organic compounds; Oxygen; Reproduction; Spermatozoa; Thermal radiation; Tracer techniques.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Caprinos e Ovinos (CNPC)	
Exemplares: 	CNPC - (2843-1) - ADD - AP - PP -  -  - 1996.00000 - DIS