Data/hora: 12/06/2024 16:58:47

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 03/05/2013
Data da última atualização: | 17/01/2024
Autoria: | FROTA, I. M. A.; LEITÃO, C. C. F.; COSTA, J. J. N.; HURK, R. van den; BRITO, I. R.; SARAIVA, M. V. A.; FIGUEIREDO, J. R.; SILVA, J. R. V.
Título: | Effects of BMP-7 and FSH on the development of goat preantral follicles and levels of mRNA for FSH-R, BMP-7 and BMP receptors after in-vitro culture.
Ano de publicação: | 2011
Fonte/Imprenta: | Animal Reproduction, v. 8, n. 1/2, p. 25-31, Jan./Jun. 2011.
Idioma: | Inglês
Conteúdo: | Abstract: This study aimed to evaluate the effects of FSH and BMP-7 on growth and on expression of FSH-R, BMP-7 and BMP receptors in cultured secondary follicles. Goat secondary follicles (~200 µm) were isolated and cultured in vitro, with 5% CO2 in air at 39°C, for 6 days in the presence of BMP-7 (50 ng/ml) supplemented or not with FSH (50 ng/ml). Follicular diameter and the formation of the antrum were evaluated before and after culture. For each treatment, at the end of culture period, groups of 6 follicles were collected and, after extraction of total RNA and cDNA synthesis, the levels of mRNA for FSH-R, BMP-7 and BMP receptors in cultured secondary follicles were quantified by real time PCR. The results showed that addition of BMP-7 or FSH to culture medium stimulated growth of secondary follicles, while addition of both BMP-7 and FSH was needed to significantly increase the percentage of follicles forming an antrum and the follicular levels of mRNA for both BMP-7 and FSH-R. For BMP receptors, FSH reduced the levels of mRNA for BMPR-IA and BMP-RII in comparison with those follicles cultured in MEM alone and supplemented with BMP-7, respectively. In conclusion, like FSH, BMP-7 affects in vitro growth of cultured secondary follicles, but it stimulates antrum formation and expression of the mRNA?s for BMP-7 and FSH-R only in presence of both BMP-7 and FSH. However, the levels of mRNA for BMP-RIA and BMP-RII are reduced in follicles cultured in medium supplemented with FSH.
Palavras-Chave: | Folículo ovariano; Folículos pre-antrais; Folítulo pré-antral; FSH.
Thesagro: | Biotecnologia; Cabra; Caprino; Fertilidade; Genética animal; Hormônio; Ovário; Reprodução animal.
Categoria do assunto: | --
Registro original: | Embrapa Caprinos e Ovinos (CNPC)

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