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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
27/08/1997 |
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Data da última atualização: |
27/08/1997 |
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Autoria: |
SIQUEIRA, J. O.; HUBBELL, D. H. |
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Título: |
Effect of organic substrates on germination and germ tube growth of vesicular-arbuscular mycorrhizal fungus spores in vitro. |
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Ano de publicação: |
1986 |
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Fonte/Imprenta: |
Pesquisa Agropecuaria Brasileira, Brasilia, v.21, n.5, p.523-527, maio. 1986. |
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Idioma: |
Inglês |
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Conteúdo: |
Vesicular-arbuscular mycorrhizal (VAM) fungi are obligate biotrophs and have not been grown in the absence of living roots. Pure culture of these fungi constitutes a critical prerequisite for intensive studies on their practical application. The effect of additional nutritional factors on spore germination and germ tube growth of surface disinfested spores of VAM fungi in vitro is reported. A range of organic substrates was tested. Most of them were inhibitory or had no effect on spores. However, small concentrations of D-galacturonic acid (1 g1-1) enhaced spore germination and 4 g 1-1 sucrose and 1% soil extract agar improved germ tube growth. The beneficial effect of soil extracts is concentrated in the protein fraction, which appears to account for the limited saprophytic ability of these fungi in soil. |
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Palavras-Chave: |
Cultura auxenica; Germinacao de esproos; Micorrizas. |
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Thesaurus Nal: |
axenic culture; mycorrhizae; spore germination. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01466naa a2200205 a 4500
001 1104344
005 1997-08-27
008 1986 bl uuuu u00u1 u #d
100 1 $aSIQUEIRA, J. O.
245 $aEffect of organic substrates on germination and germ tube growth of vesicular-arbuscular mycorrhizal fungus spores in vitro.
260 $c1986
520 $aVesicular-arbuscular mycorrhizal (VAM) fungi are obligate biotrophs and have not been grown in the absence of living roots. Pure culture of these fungi constitutes a critical prerequisite for intensive studies on their practical application. The effect of additional nutritional factors on spore germination and germ tube growth of surface disinfested spores of VAM fungi in vitro is reported. A range of organic substrates was tested. Most of them were inhibitory or had no effect on spores. However, small concentrations of D-galacturonic acid (1 g1-1) enhaced spore germination and 4 g 1-1 sucrose and 1% soil extract agar improved germ tube growth. The beneficial effect of soil extracts is concentrated in the protein fraction, which appears to account for the limited saprophytic ability of these fungi in soil.
650 $aaxenic culture
650 $amycorrhizae
650 $aspore germination
653 $aCultura auxenica
653 $aGerminacao de esproos
653 $aMicorrizas
700 1 $aHUBBELL, D. H.
773 $tPesquisa Agropecuaria Brasileira, Brasilia$gv.21, n.5, p.523-527, maio. 1986.
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