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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
04/11/1997 |
Data da última atualização: |
04/11/1997 |
Autoria: |
HONG, Y.; SAUNDERS, K.; STANLEY, J. |
Afiliação: |
Department of virus research, John Innes Centre. |
Título: |
Transactivation of dianthin transgene expression by African Cassava Mosaic Virus AC2 |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
San Diego Now York: Academic Press, 1997 |
Páginas: |
p.384-387 |
Idioma: |
Inglês |
Conteúdo: |
We have recently described a novel strategy for engineering resistance to African cassava mosaic virus (ACMV) in transgenic Nicotiana benthamiana plants using a virus-inducible promoter to control the expression of a plant ribosomew-inactivating protein (RIP) transgene (Y. Hong et al., Virology 220, 119-127, 1996). Here, we have used a potato virus X (PVX) vector to express the ACMV transactivator protein, AC2, in planta. We confirm that amplification of RIP activity in transgenic plants is mediated by AC2; disruption of AC2 expression by either the introduction of an in-frame stop codon or the deletion of 5'-terminal or 3'-terminal coding sequences reduced RIP expression to the basal level associated withPVX-infected plants. |
Categoria do assunto: |
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Marc: |
LEADER 01161naa a2200157 a 4500 001 1634624 005 1997-11-04 008 1997 bl uuuu u00u1 u #d 100 1 $aHONG, Y. 245 $aTransactivation of dianthin transgene expression by African Cassava Mosaic Virus AC2 260 $c1997 300 $ap.384-387 520 $aWe have recently described a novel strategy for engineering resistance to African cassava mosaic virus (ACMV) in transgenic Nicotiana benthamiana plants using a virus-inducible promoter to control the expression of a plant ribosomew-inactivating protein (RIP) transgene (Y. Hong et al., Virology 220, 119-127, 1996). Here, we have used a potato virus X (PVX) vector to express the ACMV transactivator protein, AC2, in planta. We confirm that amplification of RIP activity in transgenic plants is mediated by AC2; disruption of AC2 expression by either the introduction of an in-frame stop codon or the deletion of 5'-terminal or 3'-terminal coding sequences reduced RIP expression to the basal level associated withPVX-infected plants. 700 1 $aSAUNDERS, K. 700 1 $aSTANLEY, J. 773 $tSan Diego Now York: Academic Press, 1997
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Embrapa Mandioca e Fruticultura (CNPMF) |
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1. | | CSUZDI, C.; ROTA, E.; SZEDERJESI, T.; HERLOCK, E.; BROWN, G. G.; CHANG, C.-H.; DIAZ COSIN, D.; FRAGOSO, C.; JAMIESON, B. G. M.; HONG, Y.; JAMES, S. W.; PAOLETTI, M. G.; PAVLÍCEK, T.; PLISKO, D.; POP, V. V.; SHEN, H.-P. Description of a new Central African earthworm, Petroscolex centenarius gen. et sp. nov. (Crassiclitellata, Eudrilidae), celebrating the 100th birthday of Pietro Omodeo. Zootaxa, v. 4674, n. 5, p. 501-508, 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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