Data/hora: 08/06/2024 02:09:02

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 10/04/2000
Data da última atualização: | 10/04/2000
Autoria: | RAGOT, M.; HOISINGTON, D. A.
Afiliação: | Department of Genetics, North Carolina State University, Raleigh, USA, Applied Molecular Genetics Laboratory, CIMMYT, Apdo. Postal Mexico DF, Mexico.
Título: | Molecular markers for plant breeding: comparisons of RFLP and RAPD genotyping costs.
Ano de publicação: | 1993
Fonte/Imprenta: | Theoretical and Applied Genetics, v.86, n.8, p.975-984, 1993.
Idioma: | Inglês
Conteúdo: | Three molecular marker protocols, chemiluminescent restriction fragment length polymorphism (c-RFLPs), radioactivity-based restriction fragment length polymorphisms (r-RFLPs), and randomly amplified DNA polymorphisms (RAPDS) were compared in terms of cost and time efficiency. Estimates of cost of supplies and time requirements were obtained from simulations of maize (Zea mays L.) genotyping experiments utilizing protocols currently in use. The increase in total cost with increasing numbers of individuals genotyped and markers analized is higher for RAPDs than for RFLPs. RAPD s were generally found to be more cost and time efficient for studies in volving small sample sizes, while RFLPs have the advantage for large sample sizes.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (17782-1) - ADD - SP - -- - S11819 -  - S11819 - DIS