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1. | | CASANOVA, A. S.; GOMES, O.; PUPO, F. R.; HERNÁNDEZ, M. CHAILLOUX, M.; DEPESTRE, T.; HERNÁNDEZ, J. C.; MORENO, V.; LEÓN, M.; IGARZA, A.; DUARTE, C.; JIMENÉZ, I.; SANTOS, R.; NAVARRO, A.; MARRERO, A.; CARDOZA, H.; PIÑEIRO, F.; AROZARENA, N.; VILLARINO, L.; HERNÁNDEZ, M. I.; MARTÍNEZ, E.; MARTÍNEZ, M.; MUIÑO, B.; BERNAL, B.; MARTÍNEZ, H.; SALGADO, J. M.; SOCORRO, A.; CAÑET, F.; FÍ, J.; RODRÍGUEZ, A.; OSUNA, A. Manual para la producción protegida de hortalizas. La Habana: Instituto de Investigaciones Hortícolas Liliana Dimitrova, 2007. 138 p. Biblioteca(s): Embrapa Hortaliças. |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
15/02/2024 |
Data da última atualização: |
15/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
BATISTA, R. I. T. P.; DIAS, J. H.; GONÇALVES, J. D.; VERGANI, G. B.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. |
Afiliação: |
RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense; JENNIFFER HAUSCHILDT DIAS, Universidade Federal de Viçosa; JOEDSON DANTAS GONÇALVES, Universidade Estadual Paulista; GABRIEL BRUN VERGANI, Universidade Estadual Paulista; MARIA EMILIA FRANCO OLIVEIRA, Universidade Estadual Paulista; JOANNA MARIA GONÇALVES SOUZA-FABJAN, Universidade Federal Fluminense; JEFERSON FERREIRA DA FONSECA, CNPC. |
Título: |
The presence of estradiol benzoate in the cervical relaxation treatment for non-surgical embryo collection does not impair embryonic morphological quality, cryosurvival, and gene expression profile. |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
Theriogenology, v. 218, p. 208-213, Feb. 2024. |
DOI: |
10.1016/j.theriogenology.2024.01.038. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Non-surgical embryo recovery (NSER) is usually preceded by a cervical relaxation in ovine donors, based on estradiol benzoate (EB), prostaglandin (PGF), and oxytocin (OT). However, it is hypothesized that, due to poorly understood mechanisms, EB can result in embryotoxic actions. To evaluate this, 20 min before NSER superovulated sheep were induced to cervical relaxation with 0.0 (G0.0), 0.5 (G0.5), or 1.0 mg (G1.0) of EB associated with 37.5 μg of PGF 16 h before NSER and 50 IU of OT. In doing so, the efficiency and duration of the NSER procedure showed no compromise (P > 0.05). Additionally, the presence of EB did not affect (P > 0.05) the embryo's morphological quality, the development dynamics, or the abundance of transcripts associated with embryonic quality (OCT4 and NANOG), cellular stress (HSP90 and PRDX1), and apoptosis (BCL2 and BAX). A similar result (P > 0.05) was also observed when comparing embryonic cryosurvival at 24 (52.0, 52.0, and 54.0) and 48 h (60.0, 54.0, and 58.0) of in vitro culture (G0.0, G0.5, and G1.0, respectively). Thus, we can conclude that EB use does not compromise embryonic quality and cryoresistance. |
Palavras-Chave: |
Cervical dilation; Dosage effects; Drug effects; Embryo survival; Expressão gênica; Gene expression profiling; NSER; Sobrevivência embrionária. |
Thesagro: |
Criopreservação; Estradiol; Genética Animal; Genética Molecular; Ovelha; Ovino; Progesterona; Reprodução; Transferência de Embrião. |
Thesaurus NAL: |
Animal genetics; Cryopreservation; Embryo transfer; Ewes; Females; Gene expression; Molecular genetics; Oxytocin; Prostaglandins; Reproduction; Sheep. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02785naa a2200541 a 4500 001 2161981 005 2024-02-15 008 2024 bl uuuu u00u1 u #d 024 7 $a10.1016/j.theriogenology.2024.01.038.$2DOI 100 1 $aBATISTA, R. I. T. P. 245 $aThe presence of estradiol benzoate in the cervical relaxation treatment for non-surgical embryo collection does not impair embryonic morphological quality, cryosurvival, and gene expression profile.$h[electronic resource] 260 $c2024 520 $aAbstract: Non-surgical embryo recovery (NSER) is usually preceded by a cervical relaxation in ovine donors, based on estradiol benzoate (EB), prostaglandin (PGF), and oxytocin (OT). However, it is hypothesized that, due to poorly understood mechanisms, EB can result in embryotoxic actions. To evaluate this, 20 min before NSER superovulated sheep were induced to cervical relaxation with 0.0 (G0.0), 0.5 (G0.5), or 1.0 mg (G1.0) of EB associated with 37.5 μg of PGF 16 h before NSER and 50 IU of OT. In doing so, the efficiency and duration of the NSER procedure showed no compromise (P > 0.05). Additionally, the presence of EB did not affect (P > 0.05) the embryo's morphological quality, the development dynamics, or the abundance of transcripts associated with embryonic quality (OCT4 and NANOG), cellular stress (HSP90 and PRDX1), and apoptosis (BCL2 and BAX). A similar result (P > 0.05) was also observed when comparing embryonic cryosurvival at 24 (52.0, 52.0, and 54.0) and 48 h (60.0, 54.0, and 58.0) of in vitro culture (G0.0, G0.5, and G1.0, respectively). Thus, we can conclude that EB use does not compromise embryonic quality and cryoresistance. 650 $aAnimal genetics 650 $aCryopreservation 650 $aEmbryo transfer 650 $aEwes 650 $aFemales 650 $aGene expression 650 $aMolecular genetics 650 $aOxytocin 650 $aProstaglandins 650 $aReproduction 650 $aSheep 650 $aCriopreservação 650 $aEstradiol 650 $aGenética Animal 650 $aGenética Molecular 650 $aOvelha 650 $aOvino 650 $aProgesterona 650 $aReprodução 650 $aTransferência de Embrião 653 $aCervical dilation 653 $aDosage effects 653 $aDrug effects 653 $aEmbryo survival 653 $aExpressão gênica 653 $aGene expression profiling 653 $aNSER 653 $aSobrevivência embrionária 700 1 $aDIAS, J. H. 700 1 $aGONÇALVES, J. D. 700 1 $aVERGANI, G. B. 700 1 $aOLIVEIRA, M. E. F. 700 1 $aSOUZA-FABJAN, J. M. G. 700 1 $aFONSECA, J. F. da 773 $tTheriogenology$gv. 218, p. 208-213, Feb. 2024.
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