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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
24/03/2015 |
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Data da última atualização: |
24/03/2015 |
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Autoria: |
HERINGER, A. S.; STEINMACHER, D. A.; SCHIMIDT, E. C.; BOUZON, Z. L.; GUERRA, M. P. |
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Título: |
Survival and ultrastructural features of peach palm (Bactris gasipaes, Kunth) somatic embryos submitted to cryopreservation through vitrification. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Protoplasma, v. 250, p. 1185-1193, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
Bactris gasipaes (Arecaceae), also known as peach palm, was domesticated by Amazonian Indians and is cultivated for its fruit and heart-of-palm, a vegetable grown in the tree?s inner core. Currently, the conservation of this species relies on in situ conditions and field gene banks. Complementary conservation strategies, such as those based on in vitro techniques, are indicated in such cases. To establish an appropriate cryopreservation protocol, this study aimed to evaluate the ultrastructural features of B. gasipaes embryogenic cultures submitted to vitrification and subsequent cryogenic temperatures. Accordingly, somatic embryo clusters were submitted to Plant Vitrification Solution 3 (PVS3). In general, cells submitted to PVS3 had viable cell characteristics associated with apparently many mitochondria, prominent nucleus, and preserved cell walls. Cells not incubated in PVS3 did not survive after the cryogenic process in liquid nitrogen. The best incubation time for the vitrification technique was 240 min, resulting in a survival rate of 37 %. In these cases, several features were indicative of quite active cell metabolism, including intact nuclei and preserved cell walls, an apparently many of mitochondria and lipid bodies, and the presence of many starch granules and condensed chromatin. Moreover, ultrastructure analysis revealed that overall cellular structures had been preserved after cryogenic treatment, thus validating the use of vitrification in conjunction with cryopreservation of peach palm elite genotypes, as well as wild genotypes, which carry a rich pool of genes that must be conserved. MenosBactris gasipaes (Arecaceae), also known as peach palm, was domesticated by Amazonian Indians and is cultivated for its fruit and heart-of-palm, a vegetable grown in the tree?s inner core. Currently, the conservation of this species relies on in situ conditions and field gene banks. Complementary conservation strategies, such as those based on in vitro techniques, are indicated in such cases. To establish an appropriate cryopreservation protocol, this study aimed to evaluate the ultrastructural features of B. gasipaes embryogenic cultures submitted to vitrification and subsequent cryogenic temperatures. Accordingly, somatic embryo clusters were submitted to Plant Vitrification Solution 3 (PVS3). In general, cells submitted to PVS3 had viable cell characteristics associated with apparently many mitochondria, prominent nucleus, and preserved cell walls. Cells not incubated in PVS3 did not survive after the cryogenic process in liquid nitrogen. The best incubation time for the vitrification technique was 240 min, resulting in a survival rate of 37 %. In these cases, several features were indicative of quite active cell metabolism, including intact nuclei and preserved cell walls, an apparently many of mitochondria and lipid bodies, and the presence of many starch granules and condensed chromatin. Moreover, ultrastructure analysis revealed that overall cellular structures had been preserved after cryogenic treatment, thus validating the use of vitrification in conjunction with c... Mostrar Tudo |
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Palavras-Chave: |
Espécie florestal; Pupunheira. |
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Thesagro: |
Bactris Gasipaes; Criopreservação; Espécie Nativa; Palmito; Pupunha. |
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Categoria do assunto: |
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Marc: |
LEADER 02400naa a2200253 a 4500
001 2012023
005 2015-03-24
008 2013 bl uuuu u00u1 u #d
100 1 $aHERINGER, A. S.
245 $aSurvival and ultrastructural features of peach palm (Bactris gasipaes, Kunth) somatic embryos submitted to cryopreservation through vitrification.$h[electronic resource]
260 $c2013
520 $aBactris gasipaes (Arecaceae), also known as peach palm, was domesticated by Amazonian Indians and is cultivated for its fruit and heart-of-palm, a vegetable grown in the tree?s inner core. Currently, the conservation of this species relies on in situ conditions and field gene banks. Complementary conservation strategies, such as those based on in vitro techniques, are indicated in such cases. To establish an appropriate cryopreservation protocol, this study aimed to evaluate the ultrastructural features of B. gasipaes embryogenic cultures submitted to vitrification and subsequent cryogenic temperatures. Accordingly, somatic embryo clusters were submitted to Plant Vitrification Solution 3 (PVS3). In general, cells submitted to PVS3 had viable cell characteristics associated with apparently many mitochondria, prominent nucleus, and preserved cell walls. Cells not incubated in PVS3 did not survive after the cryogenic process in liquid nitrogen. The best incubation time for the vitrification technique was 240 min, resulting in a survival rate of 37 %. In these cases, several features were indicative of quite active cell metabolism, including intact nuclei and preserved cell walls, an apparently many of mitochondria and lipid bodies, and the presence of many starch granules and condensed chromatin. Moreover, ultrastructure analysis revealed that overall cellular structures had been preserved after cryogenic treatment, thus validating the use of vitrification in conjunction with cryopreservation of peach palm elite genotypes, as well as wild genotypes, which carry a rich pool of genes that must be conserved.
650 $aBactris Gasipaes
650 $aCriopreservação
650 $aEspécie Nativa
650 $aPalmito
650 $aPupunha
653 $aEspécie florestal
653 $aPupunheira
700 1 $aSTEINMACHER, D. A.
700 1 $aSCHIMIDT, E. C.
700 1 $aBOUZON, Z. L.
700 1 $aGUERRA, M. P.
773 $tProtoplasma$gv. 250, p. 1185-1193, 2013.
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Embrapa Florestas (CNPF) |
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| 2. |  | POLESI, L. G.; FRAGA, H. P. F. de; VIEIRA, L. do N.; HERINGER, A. S.; ORNELLAS, T. S.; SANTOS, H. P. dos; GUERRA, M. P.; PESCADOR, R. Chloroplast ultrastructure and hormone endogenous levels are differently affected under light and dark conditions during in vitro culture of Guadua chacoensis (Rojas) Londoño & P. M. Peterson. Acta Physiologiae Plantarum , v. 41, n. 10, p. 1-12, Jan. 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 3. | | POLESE, V.; SILVA, P. R. A.; VALE, E.; HERINGER, A. S.; SOUZA FILHO, G. A. de; SILVEIRA, V.; BALDANI, J. I.; VIDAL, M. S. The effect of sugarcane apoplastic fluid on the proteome of HCC103 strain of Herbaspirillum rubrisubalbicans. In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16., LATINAMERICAN WORKSHOP OF PGPR, 4., RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Emprapa, 2018. p. 43 Session 4, Poster III.9| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 3 | |
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