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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
22/04/2008 |
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Data da última atualização: |
16/03/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROUWS, L. F. M.; SIMÕES ARAÚJO, J. L.; HEMERLY, A. S.; BALDANI, J. I. |
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Afiliação: |
Luc F. M. Rouws, UFRRJ; Jean Luis Simões Araújo, Embrapa Agrobiologia; Adriana S. Hemerly, Instituto de Pesquisas do Jardim Botânico do RJ; José Ivo Baladani, Embrapa Agrobiologia. |
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Título: |
Validation of a Tn5 transposon mutagenesis system for Gluconacetobacter diazotrophicus through characterization of a flagellar mutant. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Archives of Microbiology, New York, v. 189, n. 4, p. 397-405, apr. 2008. |
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Idioma: |
Inglês |
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Notas: |
Parceria: UFRRJ; Instituto de Pesquisas do Jardim Botânico do Rio de Janeiro. |
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Conteúdo: |
Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium, which was originally isolated from the interior of sugarcane plants. The genome of strain PAL5 of G. diazotrophicus has been completely sequenced and a next step is the functional characterization of its genes. The aim of this study was to establish an efficient mutagenesis method, using the commercial Tn5 transposon EZ::Tn5?Tnp Transposome? (Epicentre). Up to 1 × 106 mutants per microgram of transposome were generated in a single electroporation experiment. Insertion-site flanking sequences were amplified by inverse PCR and sequenced for 31 mutants. For ten of these mutants, both insertion flanks could be identified, confirming the 9 bp duplication that is typical for Tn5 transposition. Insertions occurred in a random fashion and were genetically stable for at least 50 generations. One mutant had an insertion in a homolog of the flagellar gene flgA, and was therefore predicted to be affected in flagella-dependent traits and used to validate the applied mutagenesis methodology. This mutant lacked flagella and was non-motile on soft agar. Interestingly, it was also strongly affected in the ability to form biofilm on glass wool. |
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Palavras-Chave: |
Bactéria endofítica. |
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Thesaurus Nal: |
Gluconacetobacter diazotrophicus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01936naa a2200193 a 4500
001 1626792
005 2015-03-16
008 2008 bl uuuu u00u1 u #d
100 1 $aROUWS, L. F. M.
245 $aValidation of a Tn5 transposon mutagenesis system for Gluconacetobacter diazotrophicus through characterization of a flagellar mutant.
260 $c2008
500 $aParceria: UFRRJ; Instituto de Pesquisas do Jardim Botânico do Rio de Janeiro.
520 $aGluconacetobacter diazotrophicus is a nitrogen-fixing bacterium, which was originally isolated from the interior of sugarcane plants. The genome of strain PAL5 of G. diazotrophicus has been completely sequenced and a next step is the functional characterization of its genes. The aim of this study was to establish an efficient mutagenesis method, using the commercial Tn5 transposon EZ::Tn5?<KAN-2>Tnp Transposome? (Epicentre). Up to 1 × 106 mutants per microgram of transposome were generated in a single electroporation experiment. Insertion-site flanking sequences were amplified by inverse PCR and sequenced for 31 mutants. For ten of these mutants, both insertion flanks could be identified, confirming the 9 bp duplication that is typical for Tn5 transposition. Insertions occurred in a random fashion and were genetically stable for at least 50 generations. One mutant had an insertion in a homolog of the flagellar gene flgA, and was therefore predicted to be affected in flagella-dependent traits and used to validate the applied mutagenesis methodology. This mutant lacked flagella and was non-motile on soft agar. Interestingly, it was also strongly affected in the ability to form biofilm on glass wool.
650 $aGluconacetobacter diazotrophicus
653 $aBactéria endofítica
700 1 $aSIMÕES ARAÚJO, J. L.
700 1 $aHEMERLY, A. S.
700 1 $aBALDANI, J. I.
773 $tArchives of Microbiology, New York$gv. 189, n. 4, p. 397-405, apr. 2008.
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Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 34 | |
| 1. |  | VARGAS, L. K.; BALDANI, J. I.; HEMERLY, A. S. Association of rice cultivars with azospirillum brasilense and Burkholderia brasilensis: studies on the expression of ethylene receptors. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. SlV.24| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 14. | | NOGUEIRA, E. de M.; OLIVARES, F. L.; JAPIASSU, J. C.; VILAR, C.; VINAGRE, F.; BALDANI, J. I.; HEMERLY, A. S. Characterization of glutamine synthetase genes in sugarcane genotypes with different rates of biological nitrogen fixation. Plant Science, Ireland, v. 169, n. 5, p. 819-832, nov. 2005.| Biblioteca(s): Embrapa Agrobiologia. |
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| 15. | | CARVALHO, T. G.; ROSMAN, A. C.; GRAVITOL, C.; NOGUEIRA, E. de M.; BALDANI, J. I.; HEMERLY, A. S. genotypes with contrasting biological nitrogen fixation efficiencies differentially modulate nitrogen metabolism, auxin signaling, and microorganism perception pathways. Plants, v. 11, n. 15, 1971, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 17. | | ROUWS, L. F. M.; SCHWAB, S.; GUEDES, H. V.; TEIXEIRA, K. R. dos S.; HEMERLY, A. S.; BALDANI, J. I. Isolation of Gluconacetobacter diazotrophicus flagellar motility mutants. In: ANNUAL MEETING OF SBBq, 37., CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia. Abstracts... Águas de Lindóia: SBBq, 2008. 1 p. Parceria: UFRRJ; UFRJ; Laboratório de Biologia Molecular de Plantas-Jardim Botânico, RJ.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 18. | | BOMFIM, A. C. J. S.; CARVALHO, T. L. G.; VICENTINI, R.; BALDANI, J. I.; HEMERLY, A. S. Nitrogen signaling regulation of sugarcane devolpment mediated by the diazotrophic endochytic bacteria. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. SlV. 10| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 19. | | VINAGRE, F.; VARGAS, C.; SCHWARCZ, K.; CAVALCANTE, J.; NOGUEIRA, E. M.; BALDANI, J. I.; FERREIRA, P. C. G.; HEMERLY, A. S. SHR5: a novel plant receptor kinase involved in plant-N2-fixing endophytic bacteria association. Journal of Experimental Botany, Oxford, v. 57, n. 3, p. 559-569, feb. 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 20. | | CARVALHO, T. L. G.; THIEBAUT, F.; GAMOSA, E.; CARDOZO, A. M.; HARDOIM, R. P.; REIS, V. M.; FERREIRA, P. C. G.; HEMERLY, A, S. Expression profi le of nitrogen regulation during maize association with diazotrophic bacteria. In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16., LATINAMERICAN WORKSHOP OF PGPR, 4., RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Emprapa, 2018. p. 51 Session 4, Poster IV.4| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 34 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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