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Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
18/08/2021 |
Data da última atualização: |
19/08/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PEREZ, V. K. C.; CUSTODIO, D. A. C.; SILVA, E. M. M.; OLIVEIRA, J. de; GUIMARÃES, A. S.; BRITO, M. A. V. P.; SOUZA-FILHO, A. F.; HEINEMANN, M. B.; LAGE, A. P.; DORNELES, E. M. S. |
Afiliação: |
VERÓNICA K. C. PÉREZ, Universidade Federal de Lavras, Lavras; DIRCÉIA A. C. CUSTÓDIO, Universidade Federal de Lavras; EDUARDA M. M. SILVA, Universidade Federal de Lavras; JULIA DE OLIVEIRA, Universidade Federal de Lavras; ALESSANDRO DE SA GUIMARAES, CNPGL; MARIA A. V. P. BRITO; ANTÔNIO F. SOUZA-FILHO, Universidade de São Paulo; MARCOS B. HEINEMANN, Universidade de São Paulo; ANDREY P. LAGE, Universidade Federal de Minas Gerais; ELAINE M. S. DORNELES, Universidade Federal de Lavras. |
Título: |
Virulence factors and antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis in Brazil. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 51, p. 2111-2122, 2020. |
DOI: |
https://doi.org/10.1007/s42770-020-00363-5 |
Idioma: |
Inglês |
Conteúdo: |
This study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), ermT (9.52%), ermY (4.76%), msrA (9.52%), and mphC (9.52%) were also detected among resistant isolates. No association between virulence factors or antimicrobialresistant genes and year of isolation, geographic origin, or antimicrobial resistance profile was observed. Our results showed that S. aureus strains isolated from bovine mastitis in the four Brazilian states sampled are mainly biofilmforming and hemolytic, whereas virulence genes associated with enterotoxins, luk and tst, were less frequently observed. Moreover, a wide variety of resistance genes that confer resistance to almost all classes of antimicrobial agents approved for use in animals and humans were found. Overall, the data point to a great pathogenic potential of S. aureus associated with bovine mastitis and to the non-negligible risks to public health of staphylococcal infections from animal origin. MenosThis study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), er... Mostrar Tudo |
Palavras-Chave: |
Hemolisina; Infecção intramamária; Intramammary infection; Mastite; Staphylococci. |
Thesagro: |
Biofilme; Bovino; Doença Animal; Estafilococo. |
Thesaurus Nal: |
Biofilm; Hemolysins. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03482naa a2200373 a 4500 001 2133706 005 2021-08-19 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s42770-020-00363-5$2DOI 100 1 $aPEREZ, V. K. C. 245 $aVirulence factors and antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis in Brazil.$h[electronic resource] 260 $c2020 520 $aThis study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), ermT (9.52%), ermY (4.76%), msrA (9.52%), and mphC (9.52%) were also detected among resistant isolates. No association between virulence factors or antimicrobialresistant genes and year of isolation, geographic origin, or antimicrobial resistance profile was observed. Our results showed that S. aureus strains isolated from bovine mastitis in the four Brazilian states sampled are mainly biofilmforming and hemolytic, whereas virulence genes associated with enterotoxins, luk and tst, were less frequently observed. Moreover, a wide variety of resistance genes that confer resistance to almost all classes of antimicrobial agents approved for use in animals and humans were found. Overall, the data point to a great pathogenic potential of S. aureus associated with bovine mastitis and to the non-negligible risks to public health of staphylococcal infections from animal origin. 650 $aBiofilm 650 $aHemolysins 650 $aBiofilme 650 $aBovino 650 $aDoença Animal 650 $aEstafilococo 653 $aHemolisina 653 $aInfecção intramamária 653 $aIntramammary infection 653 $aMastite 653 $aStaphylococci 700 1 $aCUSTODIO, D. A. C. 700 1 $aSILVA, E. M. M. 700 1 $aOLIVEIRA, J. de 700 1 $aGUIMARÃES, A. S. 700 1 $aBRITO, M. A. V. P. 700 1 $aSOUZA-FILHO, A. F. 700 1 $aHEINEMANN, M. B. 700 1 $aLAGE, A. P. 700 1 $aDORNELES, E. M. S. 773 $tBrazilian Journal of Microbiology$gv. 51, p. 2111-2122, 2020.
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Embrapa Gado de Leite (CNPGL) |
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Registro Completo
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
25/04/2002 |
Data da última atualização: |
25/04/2002 |
Autoria: |
UEMURA, Y.; URBEN FILHO, G.; SPEHAR, C. R. |
Título: |
Developing optional crops to antecipate or suceed soybeans. |
Ano de publicação: |
2000 |
Fonte/Imprenta: |
In: PROJECT of sustainable agricultural development and natural resources conservation in the Brazilian Cerrado: technical report 1994-1999. Planaltina, DF: JICA: Embrapa Cerrados, 2000. Cap. 15. |
Páginas: |
p. 270-300. |
Descrição Física: |
1 CD ROM. |
Idioma: |
Inglês |
Notas: |
Editado por Edson Sano. |
Palavras-Chave: |
Sucessao de cultura. |
Thesagro: |
Cerrado; Glycine Max; Soja. |
Thesaurus NAL: |
sequential cropping; soybeans. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00769naa a2200229 a 4500 001 1564771 005 2002-04-25 008 2000 bl uuuu u00u1 u #d 100 1 $aUEMURA, Y. 245 $aDeveloping optional crops to antecipate or suceed soybeans. 260 $c2000 300 $ap. 270-300.$c1 CD ROM. 500 $aEditado por Edson Sano. 650 $asequential cropping 650 $asoybeans 650 $aCerrado 650 $aGlycine Max 650 $aSoja 653 $aSucessao de cultura 700 1 $aURBEN FILHO, G. 700 1 $aSPEHAR, C. R. 773 $tIn: PROJECT of sustainable agricultural development and natural resources conservation in the Brazilian Cerrado: technical report 1994-1999. Planaltina, DF: JICA: Embrapa Cerrados, 2000. Cap. 15.
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