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Registro Completo |
Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
17/07/2012 |
Data da última atualização: |
07/10/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MONTE, L. G.; CONCEIÇÃO, F. R.; COUTINHO, M. L.; SEIXAS, F. K.; SILVA, E. V. da; VASCONCELLOS, F. A.; CASTRO, L. A. S. de; HARTLEBEN, C. P.; DELLAGOSTIN, O. A.; ALEIXO, J. A. G. |
Afiliação: |
LEONARDO G. MONTE, UFPel; FABRICIO, R. CONCEIÇÃO, UFPel; MARIANA L. COUTINHO, UFPel; FABIANA K. SEIXAS, UFPel; EVERTON F. da SILVA, UFPel; FLÁVIA A. VASCONCELLOS, UFPel; LUIS ANTONIO SUITA DE CASTRO, CPACT; CLÁUDIA P. HARTLEBEN, UFPel; ODIR A. DELLAGOSTIN, UFPel; JOSÉ A. G. ALEIXO, UFPel. |
Título: |
Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Comparative Immunology, Microbiology and Infectious Diseases, v. 34, n. 5, p. 441-446, set. 2011. |
ISSN: |
0147-9571 |
DOI: |
https://doi.org/10.1016/j.cimid.2011.08.003 |
Idioma: |
Inglês |
Conteúdo: |
Leptospirosis is an infectious disease caused by pathogenic spirochetes of the genus Leptospira that affects humans and a wide variety of animals. Recently the genomes of Leptospira interrogans, Leptospira borgpetersenii and Leptospira biflexa species were sequenced allowing the identification of new virulence factors involved in survival and pathogenesis of bacteria. LigA and LigB are surface-exposed bacterial adhesins whose expression is correlated with the virulence of Leptospira strains. In this study, we produced and characterized five monoclonal antibodies (MAbs) against a recombinant fragment of LigB (rLigBrep) with approximately 54 kDa that comprise the portions of LigA and LigB (domains 2?7). The 5 MAbs obtained were of the IgG1 (2) and IgG2b (3) isotypes and their affinity constants for rLigBrep ranged from 7 × 107M−1 to 4 × 108M−1. The MAbs were able to react with the native antigen on the L. interrogans, L. borgpetersenii and Leptospira noguchii surfaces by indirect immunofluorescence, immunoblotting and immunoelectron microscopy. These results demonstrate that the MAbs anti-rLigBrep can be useful to complement genetic studies and to aid studies aiming understanding the role of Lig proteins in Leptospira pathogenesis and the development of Lig-based vaccines and improved diagnostic tests for leptospirosis. |
Palavras-Chave: |
Monoclonal. |
Thesagro: |
Anticorpo Monoclonal; Leptospirose. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/212910/1/12-Artigo-LeonardoMonte-Medicina-Veterinaria-A1.pdf
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Marc: |
LEADER 02283naa a2200289 a 4500 001 1928502 005 2021-10-07 008 2011 bl uuuu u00u1 u #d 022 $a0147-9571 024 7 $ahttps://doi.org/10.1016/j.cimid.2011.08.003$2DOI 100 1 $aMONTE, L. G. 245 $aMonoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions.$h[electronic resource] 260 $c2011 520 $aLeptospirosis is an infectious disease caused by pathogenic spirochetes of the genus Leptospira that affects humans and a wide variety of animals. Recently the genomes of Leptospira interrogans, Leptospira borgpetersenii and Leptospira biflexa species were sequenced allowing the identification of new virulence factors involved in survival and pathogenesis of bacteria. LigA and LigB are surface-exposed bacterial adhesins whose expression is correlated with the virulence of Leptospira strains. In this study, we produced and characterized five monoclonal antibodies (MAbs) against a recombinant fragment of LigB (rLigBrep) with approximately 54 kDa that comprise the portions of LigA and LigB (domains 2?7). The 5 MAbs obtained were of the IgG1 (2) and IgG2b (3) isotypes and their affinity constants for rLigBrep ranged from 7 × 107M−1 to 4 × 108M−1. The MAbs were able to react with the native antigen on the L. interrogans, L. borgpetersenii and Leptospira noguchii surfaces by indirect immunofluorescence, immunoblotting and immunoelectron microscopy. These results demonstrate that the MAbs anti-rLigBrep can be useful to complement genetic studies and to aid studies aiming understanding the role of Lig proteins in Leptospira pathogenesis and the development of Lig-based vaccines and improved diagnostic tests for leptospirosis. 650 $aAnticorpo Monoclonal 650 $aLeptospirose 653 $aMonoclonal 700 1 $aCONCEIÇÃO, F. R. 700 1 $aCOUTINHO, M. L. 700 1 $aSEIXAS, F. K. 700 1 $aSILVA, E. V. da 700 1 $aVASCONCELLOS, F. A. 700 1 $aCASTRO, L. A. S. de 700 1 $aHARTLEBEN, C. P. 700 1 $aDELLAGOSTIN, O. A. 700 1 $aALEIXO, J. A. G. 773 $tComparative Immunology, Microbiology and Infectious Diseases$gv. 34, n. 5, p. 441-446, set. 2011.
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Embrapa Clima Temperado (CPACT) |
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Registros recuperados : 2 | |
1. | | MONTE, L. G.; CONCEIÇÃO, F. R.; COUTINHO, M. L.; SEIXAS, F. K.; SILVA, E. V. da; VASCONCELLOS, F. A.; CASTRO, L. A. S. de; HARTLEBEN, C. P.; DELLAGOSTIN, O. A.; ALEIXO, J. A. G. Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions. Comparative Immunology, Microbiology and Infectious Diseases, v. 34, n. 5, p. 441-446, set. 2011.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Clima Temperado. |
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2. | | SA, G. L. de; PACHECO, D. de B.; MONTE, L. G.; SINNOTT, F. A.; XAVIER, M. A.; RIZZI, C.; BORSUK, S.; BERNE, M. E. A.; ANDREOTTI, R.; HARTLEBEN, c. p. Diagnostic Potential of Anti-rNcp-43 Polyclonal Antibodies for the Detection of Neospora caninum Current Microbiology, v. 68, n. 4, abril, 2014, p. 472-776Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
Biblioteca(s): Embrapa Gado de Corte. |
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Registros recuperados : 2 | |
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