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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Soja. |
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Data corrente: |
02/10/2015 |
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Data da última atualização: |
13/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; REIS, R. R.; FARIAS, J. R. B.; HARMON, F. G.; MOLINARI, H. B. C.; MOLINARI, M. D. C.; NEPOMUCENO, A. L. |
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Afiliação: |
UEL; AUTOR; AUTOR; UFV; UEL; JOSE RENATO BOUCAS FARIAS, CNPSO; U C BERKELEY - BERKELEY; HUGO BRUNO CORREA MOLINARI, CNPAE; UEL; ALEXANDRE LIMA NEPOMUCENO, CNPSO. |
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Título: |
Transcriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plos One, v. 10, n. 9, e0139051, 2015. |
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Páginas: |
16 p. |
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DOI: |
10.1371/journal.pone.0139051 |
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Idioma: |
Inglês |
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Conteúdo: |
The soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day. MenosThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean ge... Mostrar Tudo |
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Palavras-Chave: |
Drought stress; Soybean. |
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Thesagro: |
Estiagem; Resistência à seca; Seca; Soja. |
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Thesaurus Nal: |
Drought tolerance; Soybeans; Transcriptome. |
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Categoria do assunto: |
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F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166647/1/nepomuceno-plos-one.pdf
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Marc: |
LEADER 02592naa a2200361 a 4500
001 2079599
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008 2015 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pone.0139051$2DOI
100 1 $aMARCOLINO-GOMES, J.
245 $aTranscriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day.$h[electronic resource]
260 $c2015
300 $a16 p.
520 $aThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day.
650 $aDrought tolerance
650 $aSoybeans
650 $aTranscriptome
650 $aEstiagem
650 $aResistência à seca
650 $aSeca
650 $aSoja
653 $aDrought stress
653 $aSoybean
700 1 $aRODRIGUES, F. A.
700 1 $aFUGANTI-PAGLIARINI, R.
700 1 $aNAKAYAMA, T. J.
700 1 $aREIS, R. R.
700 1 $aFARIAS, J. R. B.
700 1 $aHARMON, F. G.
700 1 $aMOLINARI, H. B. C.
700 1 $aMOLINARI, M. D. C.
700 1 $aNEPOMUCENO, A. L.
773 $tPlos One$gv. 10, n. 9, e0139051, 2015.
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Registro original: |
Embrapa Soja (CNPSO) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
20/01/2011 |
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Data da última atualização: |
20/01/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
JESUS-HITZSCHKY, K. R. e; ASSIS, O. B. G. de; RODRIGUES, G. S.; NESHICH, G.; DURÁN, N.; ZACKIEWICZ, M.; BONACELLI, M. B. M.; PLONSKI, G. A.; GUTERRES, S. S. |
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Afiliação: |
KATIA REGINA E DE JESUS HITZSCHKY, CNPMA; ODILIO BENEDITO GARRIDO DE ASSIS, CNPDIA; GERALDO STACHETTI RODRIGUES, CNPMA; GORAN NESHICH, CNPTIA; Nelson Durán, Instituto de Química - UNICAMP; Mauro Zackiewicz, GEOPI/UNICAMP; Maria Beatriz M. Bonacelli, GEOPI/UNICAMP; Guilherme Ary Plonski, FEA/USP; Sílvia S. Guterres, UFRGS. |
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Título: |
Metodologia para avaliação dos impactos éticos, sociais e ambientais das nanotecnologias. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: SIMPÓSIO SOBRE INOVAÇÃO E CRIATIVIDADE CIENTÍFICA NA EMBRAPA, 2., 2010, Brasília, DF. Resumos... Brasília, DF: Embrapa, 2010. Poster 051. |
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Idioma: |
Português |
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Palavras-Chave: |
Nanotecnologia. |
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Thesagro: |
Impacto Ambiental; Impacto Social. |
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Categoria do assunto: |
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Marc: |
LEADER 00806naa a2200241 a 4500
001 1873705
005 2011-01-20
008 2010 bl --- 0-- u #d
100 1 $aJESUS-HITZSCHKY, K. R. e
245 $aMetodologia para avaliação dos impactos éticos, sociais e ambientais das nanotecnologias.
260 $c2010
650 $aImpacto Ambiental
650 $aImpacto Social
653 $aNanotecnologia
700 1 $aASSIS, O. B. G. de
700 1 $aRODRIGUES, G. S.
700 1 $aNESHICH, G.
700 1 $aDURÁN, N.
700 1 $aZACKIEWICZ, M.
700 1 $aBONACELLI, M. B. M.
700 1 $aPLONSKI, G. A.
700 1 $aGUTERRES, S. S.
773 $tIn: SIMPÓSIO SOBRE INOVAÇÃO E CRIATIVIDADE CIENTÍFICA NA EMBRAPA, 2., 2010, Brasília, DF. Resumos... Brasília, DF: Embrapa, 2010. Poster 051.
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Embrapa Meio Ambiente (CNPMA) |
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