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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
06/04/2004 |
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Data da última atualização: |
27/07/2007 |
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Autoria: |
ALMEIDA, A. M. R.; SAKAI, J.; HANADA, K.; OLIVEIRA, T. G.; BELINTANI, P.; KITAJIMA, E. W.; NORA, P. S.; NOVAES, T. G. |
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Título: |
Biological and molecular characterization of an isolate of tobacco streak virus isolated from bud blight soybeans in Brazil. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
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Páginas: |
p. 229-230. |
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Série: |
(Embrapa Soja. Documentos, 228). |
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Idioma: |
Inglês |
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Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
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Conteúdo: |
Soybean plants with symptoms of dwarfing and bud blight were observed in commercial fields near Wenceslau Braz county, State of Paraná, Brazil. The plants were used for grafting and for mechanical inoculation. Symptoms appeared 10-12 days after the inoculation or grafting and were identical to those observed in the field. Preliminary examination of leaf tissues from diseased plants by electron microscopy revealed the presence of spherical particles ca. 30 nm diameter. The host range and serological tests identified as tobacco streak virus (TSV). Purified virus showed three peaks in sucrose gradient and electron microscopy revealed spherical particles with 30 nm. Coat protein has a Mr of 29.880 kDa determined in SDS-PAGE. Polyclonal antibodies were obtained against this virus. With specific primers designed based on sequence of the TSV (Cornelissen et al., 1984 Nucleic Acids Res: 12, 277-282) a fragment of 1028 nt was RT-PCR amplified, cloned and sequenced. It contained an open reading frame with 717 nt and 238 deduced amino acids, identified and associated to the coat protein and a 3' untranslated region with 287 nucleotides. Sequence comparison of the nucleotides and predicted amino acids with sequence of TSV CP from white clover (GenBank NC003845, CAA25133) showed identities of 81% and 83%, respectively. A putative Zinc-finger motif was found in the coat protein N-terminus and the 3' untranslated region (UTR) revelead a potential stem-loop structure interpersed with AUGC-motifs, a structure which may signal binding of coat protein and activation of genome replication. The phylogenetic analysis grouped thi siolate of TSV and other TSV isolates in the same branch in 96% of the replicates.This is the first report of molecular characterization of tobacco streak virus isolated from soybeans. It is proposed that this isolate be considered a strain of TSV and named TSV-BR. MenosSoybean plants with symptoms of dwarfing and bud blight were observed in commercial fields near Wenceslau Braz county, State of Paraná, Brazil. The plants were used for grafting and for mechanical inoculation. Symptoms appeared 10-12 days after the inoculation or grafting and were identical to those observed in the field. Preliminary examination of leaf tissues from diseased plants by electron microscopy revealed the presence of spherical particles ca. 30 nm diameter. The host range and serological tests identified as tobacco streak virus (TSV). Purified virus showed three peaks in sucrose gradient and electron microscopy revealed spherical particles with 30 nm. Coat protein has a Mr of 29.880 kDa determined in SDS-PAGE. Polyclonal antibodies were obtained against this virus. With specific primers designed based on sequence of the TSV (Cornelissen et al., 1984 Nucleic Acids Res: 12, 277-282) a fragment of 1028 nt was RT-PCR amplified, cloned and sequenced. It contained an open reading frame with 717 nt and 238 deduced amino acids, identified and associated to the coat protein and a 3' untranslated region with 287 nucleotides. Sequence comparison of the nucleotides and predicted amino acids with sequence of TSV CP from white clover (GenBank NC003845, CAA25133) showed identities of 81% and 83%, respectively. A putative Zinc-finger motif was found in the coat protein N-terminus and the 3' untranslated region (UTR) revelead a potential stem-loop structure interpersed with AUGC-m... Mostrar Tudo |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02968naa a2200241 a 4500
001 1466812
005 2007-07-27
008 2004 bl uuuu u00u1 u #d
100 1 $aALMEIDA, A. M. R.
245 $aBiological and molecular characterization of an isolate of tobacco streak virus isolated from bud blight soybeans in Brazil.
260 $c2004
300 $ap. 229-230.
490 $a(Embrapa Soja. Documentos, 228).
500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
520 $aSoybean plants with symptoms of dwarfing and bud blight were observed in commercial fields near Wenceslau Braz county, State of Paraná, Brazil. The plants were used for grafting and for mechanical inoculation. Symptoms appeared 10-12 days after the inoculation or grafting and were identical to those observed in the field. Preliminary examination of leaf tissues from diseased plants by electron microscopy revealed the presence of spherical particles ca. 30 nm diameter. The host range and serological tests identified as tobacco streak virus (TSV). Purified virus showed three peaks in sucrose gradient and electron microscopy revealed spherical particles with 30 nm. Coat protein has a Mr of 29.880 kDa determined in SDS-PAGE. Polyclonal antibodies were obtained against this virus. With specific primers designed based on sequence of the TSV (Cornelissen et al., 1984 Nucleic Acids Res: 12, 277-282) a fragment of 1028 nt was RT-PCR amplified, cloned and sequenced. It contained an open reading frame with 717 nt and 238 deduced amino acids, identified and associated to the coat protein and a 3' untranslated region with 287 nucleotides. Sequence comparison of the nucleotides and predicted amino acids with sequence of TSV CP from white clover (GenBank NC003845, CAA25133) showed identities of 81% and 83%, respectively. A putative Zinc-finger motif was found in the coat protein N-terminus and the 3' untranslated region (UTR) revelead a potential stem-loop structure interpersed with AUGC-motifs, a structure which may signal binding of coat protein and activation of genome replication. The phylogenetic analysis grouped thi siolate of TSV and other TSV isolates in the same branch in 96% of the replicates.This is the first report of molecular characterization of tobacco streak virus isolated from soybeans. It is proposed that this isolate be considered a strain of TSV and named TSV-BR.
700 1 $aSAKAI, J.
700 1 $aHANADA, K.
700 1 $aOLIVEIRA, T. G.
700 1 $aBELINTANI, P.
700 1 $aKITAJIMA, E. W.
700 1 $aNORA, P. S.
700 1 $aNOVAES, T. G.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
19/08/2020 |
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Data da última atualização: |
19/08/2020 |
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Autoria: |
CAMPOS, O. F. de; MIRANDA, J. E. C. de (ed.). |
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Afiliação: |
ORIEL FAJARDO DE CAMPOS; JOAO EUSTAQUIO CABRAL DE MIRANDA, CNPGL. |
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Título: |
Gado de leite: o produtor pergunta, a Embrapa responde. |
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Edição: |
4. ed. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Brasília, DF: Embrapa, 2013. |
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Série: |
(Coleção 500 perguntas 500 respostas). |
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ISBN: |
978-85-7035-261-3 |
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Idioma: |
Português |
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Notas: |
E-book no formato epub. |
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Conteúdo: |
Introdução; Cria e recria de bezerras e novilhas; Alimentação e manejo de vacas e touros; Recursos forrageiros; Reprodução; Melhoramento genético animal; Saúde animal; Mastide e qualidade do leite; Produção orgânica de leite; Gerenciamento da atividade leiteira; Bem-estar animal; Instalações, ambiência e manejo de dejetos. |
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Thesagro: |
Bovinocultura; Gado Leiteiro; Manejo; Melhoramento Genético Animal; Nutrição Animal; Pastagem; Planta Forrageira; Reprodução Animal. |
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Thesaurus NAL: |
Dairy cattle; Milk. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/215422/1/500-perguntas-gado-leite.epub
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Marc: |
LEADER 01149nam a2200289 a 4500
001 2124435
005 2020-08-19
008 2013 bl uuuu 00u1 u #d
020 $a978-85-7035-261-3
100 1 $aCAMPOS, O. F. de
245 $aGado de leite$bo produtor pergunta, a Embrapa responde.$h[electronic resource]
250 $a4. ed.
260 $aBrasília, DF: Embrapa$c2013
490 $a(Coleção 500 perguntas 500 respostas).
500 $aE-book no formato epub.
520 $aIntrodução; Cria e recria de bezerras e novilhas; Alimentação e manejo de vacas e touros; Recursos forrageiros; Reprodução; Melhoramento genético animal; Saúde animal; Mastide e qualidade do leite; Produção orgânica de leite; Gerenciamento da atividade leiteira; Bem-estar animal; Instalações, ambiência e manejo de dejetos.
650 $aDairy cattle
650 $aMilk
650 $aBovinocultura
650 $aGado Leiteiro
650 $aManejo
650 $aMelhoramento Genético Animal
650 $aNutrição Animal
650 $aPastagem
650 $aPlanta Forrageira
650 $aReprodução Animal
700 1 $aMIRANDA, J. E. C. de
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Embrapa Unidades Centrais (AI-SEDE) |
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