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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Pecuária Sul. |
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Data corrente: |
16/03/2020 |
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Data da última atualização: |
20/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HAEHLING, M. B. V.; CRUVINEL, G. G.; TOSCANO, J. H. B.; GIRALDELO, L. A.; SANTOS, I. B. dos; ESTEVES, S. N.; BENAVIDES, M. V.; BARIONI JUNIOR, W.; NICIURA, S. C. M.; CHAGAS, A. C. de S. |
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Afiliação: |
MAREI BORSCH VON HAEHLING, UNESP; GIOVANNA GABRIELLE CRUVINEL, UNICEP; JOÃO HENRIQUE BARBOSA TOSCANO, UNESP; LUCIANA APARECIDA GIRALDELO, UNICEP; ISABELLA BARBOSA DOS SANTOS, UNESP; SERGIO NOVITA ESTEVES, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; WALDOMIRO BARIONI JUNIOR, CPPSE; SIMONE CRISTINA MEO NICIURA, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Four single nucleotide polymorphisms (SNPs) are associated with resistance and resilience to Haemonchus contortus in Brazilian Morada Nova sheep. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 279, 2020, 109053. |
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Páginas: |
11 p. |
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ISSN: |
0304-4017 |
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DOI: |
https://doi.org/10.1016/j.vetpar.2020.109053 |
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Idioma: |
Inglês |
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Conteúdo: |
Gastrointestinal nematodes are a major constraint in sheep production. Breeding for resistance has proven to be an effective and feasible approach to address this problem. The use and investigation of genetic markers for resistance traits could accelerate genetic progress and lead to a better understanding of underlying molecular mechanisms. Thus, the aim of this study was to evaluate if five single nucleotide polymorphisms SNPs OAR2_14765360, OAR6_81718546, OAR11_62887032, OAR12_69606944 and OAR15_59871543 are associated with resistance and resilience traits in a flock of the Morada Nova sheep breed. Lambs were submitted to two consecutive parasite challenges by oral infection with 4000 infective larvae L3) of Haemonchus contortus. Fecal egg counts (FEC), packed cell volume (PVC) and body weight were measured every one or two weeks for 42 days in each trial. DNA samples from 287 lambs, 131 ewes and 4 rams were amplified by ARMS-PCR or PCR-RFLP and genotypes were determined. Analysis of variance (ANOVA) was used for association analyses between genotypes and phenotypes. In case of significant association, the allele substitution effect was calculated based on a linear model. OAR2_14765360 and OAR12_69606944 were associated with FEC, and OAR12_69606944 also had significant effects on PCV and weight gain, showing favourable associations of the CC genotype with all evaluated traits. Both OAR6_81718546 and OAR11_62887032 were associated with weight gain, and OAR6_81718546 had an additional effect on PCV. OAR15_59871543 was not polymorphic in the population. OAR6_81718546 and OAR12_69606944 presented significant allele substitution effects of -1.06 ± 0.52 kg for the T allele on final body weight and 0.74 ± 0.32 for the C allele in PCV of the same sampling date, respectively. This is the first report of SNPs associated with gastrointestinal nematode resistance in this sheep breed. Our findings support the existence of quantitatice trait loci (QTL) for resistance and resilience in linkage disequilibrium with the polymorphic SNPs and suggest their future use for explorations of these traits in Morada Nova sheep. MenosGastrointestinal nematodes are a major constraint in sheep production. Breeding for resistance has proven to be an effective and feasible approach to address this problem. The use and investigation of genetic markers for resistance traits could accelerate genetic progress and lead to a better understanding of underlying molecular mechanisms. Thus, the aim of this study was to evaluate if five single nucleotide polymorphisms SNPs OAR2_14765360, OAR6_81718546, OAR11_62887032, OAR12_69606944 and OAR15_59871543 are associated with resistance and resilience traits in a flock of the Morada Nova sheep breed. Lambs were submitted to two consecutive parasite challenges by oral infection with 4000 infective larvae L3) of Haemonchus contortus. Fecal egg counts (FEC), packed cell volume (PVC) and body weight were measured every one or two weeks for 42 days in each trial. DNA samples from 287 lambs, 131 ewes and 4 rams were amplified by ARMS-PCR or PCR-RFLP and genotypes were determined. Analysis of variance (ANOVA) was used for association analyses between genotypes and phenotypes. In case of significant association, the allele substitution effect was calculated based on a linear model. OAR2_14765360 and OAR12_69606944 were associated with FEC, and OAR12_69606944 also had significant effects on PCV and weight gain, showing favourable associations of the CC genotype with all evaluated traits. Both OAR6_81718546 and OAR11_62887032 were associated with weight gain, and OAR6_81718546 had an... Mostrar Tudo |
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Palavras-Chave: |
Host resistance; Morada Nova sheep; Parasite challenge; SNPs. |
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Thesaurus Nal: |
Genetic markers. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03156naa a2200325 a 4500
001 2121271
005 2020-04-20
008 2020 bl uuuu u00u1 u #d
022 $a0304-4017
024 7 $ahttps://doi.org/10.1016/j.vetpar.2020.109053$2DOI
100 1 $aHAEHLING, M. B. V.
245 $aFour single nucleotide polymorphisms (SNPs) are associated with resistance and resilience to Haemonchus contortus in Brazilian Morada Nova sheep.$h[electronic resource]
260 $c2020
300 $a11 p.
520 $aGastrointestinal nematodes are a major constraint in sheep production. Breeding for resistance has proven to be an effective and feasible approach to address this problem. The use and investigation of genetic markers for resistance traits could accelerate genetic progress and lead to a better understanding of underlying molecular mechanisms. Thus, the aim of this study was to evaluate if five single nucleotide polymorphisms SNPs OAR2_14765360, OAR6_81718546, OAR11_62887032, OAR12_69606944 and OAR15_59871543 are associated with resistance and resilience traits in a flock of the Morada Nova sheep breed. Lambs were submitted to two consecutive parasite challenges by oral infection with 4000 infective larvae L3) of Haemonchus contortus. Fecal egg counts (FEC), packed cell volume (PVC) and body weight were measured every one or two weeks for 42 days in each trial. DNA samples from 287 lambs, 131 ewes and 4 rams were amplified by ARMS-PCR or PCR-RFLP and genotypes were determined. Analysis of variance (ANOVA) was used for association analyses between genotypes and phenotypes. In case of significant association, the allele substitution effect was calculated based on a linear model. OAR2_14765360 and OAR12_69606944 were associated with FEC, and OAR12_69606944 also had significant effects on PCV and weight gain, showing favourable associations of the CC genotype with all evaluated traits. Both OAR6_81718546 and OAR11_62887032 were associated with weight gain, and OAR6_81718546 had an additional effect on PCV. OAR15_59871543 was not polymorphic in the population. OAR6_81718546 and OAR12_69606944 presented significant allele substitution effects of -1.06 ± 0.52 kg for the T allele on final body weight and 0.74 ± 0.32 for the C allele in PCV of the same sampling date, respectively. This is the first report of SNPs associated with gastrointestinal nematode resistance in this sheep breed. Our findings support the existence of quantitatice trait loci (QTL) for resistance and resilience in linkage disequilibrium with the polymorphic SNPs and suggest their future use for explorations of these traits in Morada Nova sheep.
650 $aGenetic markers
653 $aHost resistance
653 $aMorada Nova sheep
653 $aParasite challenge
653 $aSNPs
700 1 $aCRUVINEL, G. G.
700 1 $aTOSCANO, J. H. B.
700 1 $aGIRALDELO, L. A.
700 1 $aSANTOS, I. B. dos
700 1 $aESTEVES, S. N.
700 1 $aBENAVIDES, M. V.
700 1 $aBARIONI JUNIOR, W.
700 1 $aNICIURA, S. C. M.
700 1 $aCHAGAS, A. C. de S.
773 $tVeterinary Parasitology$gv. 279, 2020, 109053.
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Embrapa Pecuária Sudeste (CPPSE) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
05/12/2023 |
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Data da última atualização: |
05/12/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SPACKI, K. de C.; NOVI, D. M. P.; DURIGON, D. C.; FRAGA, F. C.; SANTOS, L. F. O.; HELM, C. V.; LIMA, E. A. de; PERALTA, R. A.; MOREIRA, R. de F. P. M.; CORRÊA, R. C. G.; BRACHT, A.; PERALTA, R. M. |
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Afiliação: |
KAMILA DE CASSIA SPACKI, UNIVERSIDADE ESTADUAL DE MARINGÁ; DANIELLY MARIA PAIXÃO NOVI, UNIVERSIDADE ESTADUAL DE MARINGÁ; DANIELE COCCO DURIGON, UNIVERSIDADE FEDERAL DE SANTA CATARINA; FERNANDA CRISTINA FRAGA, UNIVERSIDADE FEDERAL DE SANTA CATARINA; LUIS FELIPE OLIVA SANTOS, UNIVERSIDADE ESTADUAL DE MARINGÁ; CRISTIANE VIEIRA HELM, CNPF; EDSON ALVES DE LIMA, CNPF; ROSELY APARECIDA PERALTA, UNIVERSIDADE FEDERAL DE SANTA CATARINA; REGINA DE FATIMA PERALTA MUNIZ MOREIRA, UNIVERSIDADE FEDERAL DE SANTA CATARINA; RUBIA CARVALHO GOMES CORRÊA, CENTRO UNIVERSITÁRIO DE MARINGÁ; ADELAR BRACHT, UNIVERSIDADE ESTADUAL DE MARINGÁ; ROSANE MARINA PERALTA, UNIVERSIDADE ESTADUAL DE MARINGÁ. |
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Título: |
Improving enzymatic saccharification of peach palm (Bactris gasipaes) waste by biological pretreatment with Pleurotus ostreatus. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS E NUTRIÇÃO, 15., 2023, Campinas. Caderno de resumos [...]. Campinas: Galoá Science, 2023. |
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Páginas: |
p. 109-110. |
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ISBN: |
978-65-89463-70-2 |
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Idioma: |
Português |
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Notas: |
SLACAN 2023. |
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Conteúdo: |
Popularly known as pupunha, Bactris gasipaes Kunth is a palm tree native to the Amazon region whose planting has extended to the Southern and Southeastern states of Brazil. The production and consumption of peach palm generate a huge volume of by-products. It is estimated that approximately 84% of the total weight of the palm are lignocellulosic wastes. The white-rot fungus Pleurotus ostreatus was used for biological pretreatment of peach palm lignocellulosic wastes (inner sheath and peel) for improving the enzymatic saccharification and production of glucose. Non-treated and pre-treated B. gasipaes inner sheath and peel were submitted to hydrolysis by a commercial cellulase preparation from T. reesei. The amounts of total reducing sugars and glucose obtained from the 30d-pretreated inner sheath were seven and five times superior, respectively, when compared to those obtained with the non-pretreated inner sheath. However, the pre-treatment did not cause any improvement in the saccharification of the B. gasipaes peel. Scanning electronic microscopy of lignocellulosic fibers was done to verify the structural changes caused by the biological pretreatments. Upon the biological pretreatment the lignocellulosic structures of the inner sheath were substantially modified to less ordered ones. The main features of the modifications were detachment of the fibers, cell wall collapse and, in several cases, formation of pores in the cell wall surfaces. The peel lignocellulosic fibers showed more ordered fibrils and no modification was observed after pre-treatment. In conclusion, P. ostreatus pretreatment considerably improved saccharification of the B. gasipaes inner sheath (7-fold), but ´pretreatment of the peel was largely ineffective. MenosPopularly known as pupunha, Bactris gasipaes Kunth is a palm tree native to the Amazon region whose planting has extended to the Southern and Southeastern states of Brazil. The production and consumption of peach palm generate a huge volume of by-products. It is estimated that approximately 84% of the total weight of the palm are lignocellulosic wastes. The white-rot fungus Pleurotus ostreatus was used for biological pretreatment of peach palm lignocellulosic wastes (inner sheath and peel) for improving the enzymatic saccharification and production of glucose. Non-treated and pre-treated B. gasipaes inner sheath and peel were submitted to hydrolysis by a commercial cellulase preparation from T. reesei. The amounts of total reducing sugars and glucose obtained from the 30d-pretreated inner sheath were seven and five times superior, respectively, when compared to those obtained with the non-pretreated inner sheath. However, the pre-treatment did not cause any improvement in the saccharification of the B. gasipaes peel. Scanning electronic microscopy of lignocellulosic fibers was done to verify the structural changes caused by the biological pretreatments. Upon the biological pretreatment the lignocellulosic structures of the inner sheath were substantially modified to less ordered ones. The main features of the modifications were detachment of the fibers, cell wall collapse and, in several cases, formation of pores in the cell wall surfaces. The peel lignocellulosic fibers sho... Mostrar Tudo |
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Palavras-Chave: |
Bioeconomia circular; Circular bioeconomy; Enzimatic saccharification; Resíduos; Scarificação enzimática. |
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Thesagro: |
Bactris Gasipaes; Subproduto. |
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Thesaurus NAL: |
Pleurotus ostreatus. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 02977nam a2200373 a 4500
001 2159150
005 2023-12-05
008 2023 bl uuuu u00u1 u #d
020 $a978-65-89463-70-2
100 1 $aSPACKI, K. de C.
245 $aImproving enzymatic saccharification of peach palm (Bactris gasipaes) waste by biological pretreatment with Pleurotus ostreatus.$h[electronic resource]
260 $aIn: SIMPÓSIO LATINO AMERICANO DE CIÊNCIA DE ALIMENTOS E NUTRIÇÃO, 15., 2023, Campinas. Caderno de resumos [...]. Campinas: Galoá Science$c2023
300 $ap. 109-110.
500 $aSLACAN 2023.
520 $aPopularly known as pupunha, Bactris gasipaes Kunth is a palm tree native to the Amazon region whose planting has extended to the Southern and Southeastern states of Brazil. The production and consumption of peach palm generate a huge volume of by-products. It is estimated that approximately 84% of the total weight of the palm are lignocellulosic wastes. The white-rot fungus Pleurotus ostreatus was used for biological pretreatment of peach palm lignocellulosic wastes (inner sheath and peel) for improving the enzymatic saccharification and production of glucose. Non-treated and pre-treated B. gasipaes inner sheath and peel were submitted to hydrolysis by a commercial cellulase preparation from T. reesei. The amounts of total reducing sugars and glucose obtained from the 30d-pretreated inner sheath were seven and five times superior, respectively, when compared to those obtained with the non-pretreated inner sheath. However, the pre-treatment did not cause any improvement in the saccharification of the B. gasipaes peel. Scanning electronic microscopy of lignocellulosic fibers was done to verify the structural changes caused by the biological pretreatments. Upon the biological pretreatment the lignocellulosic structures of the inner sheath were substantially modified to less ordered ones. The main features of the modifications were detachment of the fibers, cell wall collapse and, in several cases, formation of pores in the cell wall surfaces. The peel lignocellulosic fibers showed more ordered fibrils and no modification was observed after pre-treatment. In conclusion, P. ostreatus pretreatment considerably improved saccharification of the B. gasipaes inner sheath (7-fold), but ´pretreatment of the peel was largely ineffective.
650 $aPleurotus ostreatus
650 $aBactris Gasipaes
650 $aSubproduto
653 $aBioeconomia circular
653 $aCircular bioeconomy
653 $aEnzimatic saccharification
653 $aResíduos
653 $aScarificação enzimática
700 1 $aNOVI, D. M. P.
700 1 $aDURIGON, D. C.
700 1 $aFRAGA, F. C.
700 1 $aSANTOS, L. F. O.
700 1 $aHELM, C. V.
700 1 $aLIMA, E. A. de
700 1 $aPERALTA, R. A.
700 1 $aMOREIRA, R. de F. P. M.
700 1 $aCORRÊA, R. C. G.
700 1 $aBRACHT, A.
700 1 $aPERALTA, R. M.
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