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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
27/06/1995 |
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Data da última atualização: |
27/06/1995 |
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Autoria: |
HAAHTELA, K.; LAAKSO, T.; LASSILA, E. L. N.; RONKKO, R.; KORHONEN, T. K. |
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Título: |
Interactions between N2-fixing enteric bacteria and grasses. |
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Ano de publicação: |
1988 |
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Fonte/Imprenta: |
Symbiosis, v.6, p.139-150, 1988. |
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Idioma: |
Português |
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Palavras-Chave: |
DIAZOTROFO; REVISAO. |
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Thesagro: |
Bactéria; Infecção; Lectina; Microscopia Eletrônica. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00586naa a2200229 a 4500
001 1610190
005 1995-06-27
008 1988 bl --- 0-- u #d
100 1 $aHAAHTELA, K.
245 $aInteractions between N2-fixing enteric bacteria and grasses.
260 $c1988
650 $aBactéria
650 $aInfecção
650 $aLectina
650 $aMicroscopia Eletrônica
653 $aDIAZOTROFO
653 $aREVISAO
700 1 $aLAAKSO, T.
700 1 $aLASSILA, E. L. N.
700 1 $aRONKKO, R.
700 1 $aKORHONEN, T. K.
773 $tSymbiosis$gv.6, p.139-150, 1988.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
03/05/2005 |
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Data da última atualização: |
16/08/2018 |
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Autoria: |
KÖRBES, A. P.; DROSTE, A. |
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Afiliação: |
Ana Paula Körbes, Universidade do Vale do Rio dos Sinos - Unisinos/Laboratório de Cultura de Tecidos Vegetais; Annette Droste, Universidade do Vale do Rio dos Sinos - Unisinos/Laboratório de Cultura de Tecidos Vegetais. |
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Título: |
Carbon sources and polyethylene glycol on soybean somatic embryo conversion. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 40, n. 3, p. 211-216, mar. 2005 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Fontes de carbono e polietilenoglicol na conversão de embriões somáticos de soja. |
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Conteúdo: |
Somatic embryogenesis is an efficient method for the production of target cells for soybean genetic transformation. However, this method still offers low percentages of plant regeneration, and perhaps is related to the maturation process and high morphological abnormalities of the matured embryos. This study aimed to identify a maturation medium that could contribute to the outcome of more efficient plant regeneration results. Embryogenic clusters, derived from cotyledons of immature seeds of the soybean cultivars Bragg and IAS5, were used as starting material for embryos development. Different maturation media were tested by using 6% maltose, 3% sucrose or 6% sucrose, combined with or without 25 g L-1 of the osmotic regulator polyethylene glycol (PEG-8000). The histodifferentiated embryos were quantified and classified in morphological types. Percentages of converted embryos were analyzed. Cultivar Bragg resulted in higher matured embryo quantities, but lower percentages were obtained for the conversion in comparison to cultivar IAS5. While the addition of PEG did not affect the number of embryos converted into plants, 6% sucrose enhanced the conversion percent significantly. |
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Palavras-Chave: |
osmotic regulation; PEG-8000; regulação osmótica. |
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Thesagro: |
Glycine Max; Sacarose. |
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Thesaurus NAL: |
sucrose. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/107899/1/Carbon.pdf
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Marc: |
LEADER 01906naa a2200217 a 4500
001 1113872
005 2018-08-16
008 2005 bl uuuu u00u1 u #d
100 1 $aKÖRBES, A. P.
245 $aCarbon sources and polyethylene glycol on soybean somatic embryo conversion.
260 $c2005
500 $aTítulo em português: Fontes de carbono e polietilenoglicol na conversão de embriões somáticos de soja.
520 $aSomatic embryogenesis is an efficient method for the production of target cells for soybean genetic transformation. However, this method still offers low percentages of plant regeneration, and perhaps is related to the maturation process and high morphological abnormalities of the matured embryos. This study aimed to identify a maturation medium that could contribute to the outcome of more efficient plant regeneration results. Embryogenic clusters, derived from cotyledons of immature seeds of the soybean cultivars Bragg and IAS5, were used as starting material for embryos development. Different maturation media were tested by using 6% maltose, 3% sucrose or 6% sucrose, combined with or without 25 g L-1 of the osmotic regulator polyethylene glycol (PEG-8000). The histodifferentiated embryos were quantified and classified in morphological types. Percentages of converted embryos were analyzed. Cultivar Bragg resulted in higher matured embryo quantities, but lower percentages were obtained for the conversion in comparison to cultivar IAS5. While the addition of PEG did not affect the number of embryos converted into plants, 6% sucrose enhanced the conversion percent significantly.
650 $asucrose
650 $aGlycine Max
650 $aSacarose
653 $aosmotic regulation
653 $aPEG-8000
653 $aregulação osmótica
700 1 $aDROSTE, A.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 40, n. 3, p. 211-216, mar. 2005
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Embrapa Unidades Centrais (AI-SEDE) |
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