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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
30/10/2020 |
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Data da última atualização: |
30/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HAACH, V.; GAVA, D.; CANTAO, M. E.; SCHAEFER, R. |
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Afiliação: |
VANESSA HAACH, UFRGS; DANIELLE GAVA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; REJANE SCHAEFER, CNPSA. |
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Título: |
Evaluation of two multiplex RT-PCR assays for detection and subtype differentiation of Brazilian swine influenza viruses. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 51, n. 3, p. 1447-1451, Sep 2020. |
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DOI: |
https://doi.org/10.1007/s42770-020-00250-z |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Influenza A virus (IAV) subtypes H1N1, H1N2, and H3N2 are endemic in swine herds in most pork producing countries; however, the viruses circulating in different geographic regions are antigenically and genetically distinct. In this sense, the availability of a rapid diagnostic assay to detect locally adapted IAVs and discriminate the virus subtype in clinical samples from swine is extremely important for monitoring and control of the disease. This study describes the development and validation of a multiplex RT-PCR assay for detection and subtyping of IAV from pigs. The analytical and diagnostic specificity of the assays was 100% (94.3-100.0, CI 95%), and the limit of detection was 10-3 TCID50/mL. A total of 100 samples (IAV isolates and clinical specimens) were tested, and the virus subtype was determined for 80 samples (80%; 71.1-86.7, CI 95%). From these, 50% were H1N1, 22.5% were H1N2, and 7.5% were H3N2. Partial subtyping was determined for 8.75% samples (H1pdmNx and HxN2). Additionally, mixed infections with two virus subtypes (H1N2 + H3N2 and H1N1pdm + H1pdmN2; 2.5%) and reassortant viruses (H1pdmN2, 6.25%; and H1N1hu, 2.5%) were detected by the assay. A rapid detection of the most prevalent IAV subtypes and lineages in swine is provided by the assays developed here, improving the IAV diagnosis in Brazilian laboratories, and contributing to the IAV monitoring. |
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Palavras-Chave: |
Multiplex RT-PCR; RT-PCR multiplex; Subtipagem; Vírus Influenza. |
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Thesagro: |
Diagnostico; Suíno. |
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Thesaurus Nal: |
Disease diagnosis; Influenza A virus; Polymerase chain reaction; Swine. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02291naa a2200289 a 4500
001 2126156
005 2020-10-30
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42770-020-00250-z$2DOI
100 1 $aHAACH, V.
245 $aEvaluation of two multiplex RT-PCR assays for detection and subtype differentiation of Brazilian swine influenza viruses.$h[electronic resource]
260 $c2020
520 $aAbstract: Influenza A virus (IAV) subtypes H1N1, H1N2, and H3N2 are endemic in swine herds in most pork producing countries; however, the viruses circulating in different geographic regions are antigenically and genetically distinct. In this sense, the availability of a rapid diagnostic assay to detect locally adapted IAVs and discriminate the virus subtype in clinical samples from swine is extremely important for monitoring and control of the disease. This study describes the development and validation of a multiplex RT-PCR assay for detection and subtyping of IAV from pigs. The analytical and diagnostic specificity of the assays was 100% (94.3-100.0, CI 95%), and the limit of detection was 10-3 TCID50/mL. A total of 100 samples (IAV isolates and clinical specimens) were tested, and the virus subtype was determined for 80 samples (80%; 71.1-86.7, CI 95%). From these, 50% were H1N1, 22.5% were H1N2, and 7.5% were H3N2. Partial subtyping was determined for 8.75% samples (H1pdmNx and HxN2). Additionally, mixed infections with two virus subtypes (H1N2 + H3N2 and H1N1pdm + H1pdmN2; 2.5%) and reassortant viruses (H1pdmN2, 6.25%; and H1N1hu, 2.5%) were detected by the assay. A rapid detection of the most prevalent IAV subtypes and lineages in swine is provided by the assays developed here, improving the IAV diagnosis in Brazilian laboratories, and contributing to the IAV monitoring.
650 $aDisease diagnosis
650 $aInfluenza A virus
650 $aPolymerase chain reaction
650 $aSwine
650 $aDiagnostico
650 $aSuíno
653 $aMultiplex RT-PCR
653 $aRT-PCR multiplex
653 $aSubtipagem
653 $aVírus Influenza
700 1 $aGAVA, D.
700 1 $aCANTAO, M. E.
700 1 $aSCHAEFER, R.
773 $tBrazilian Journal of Microbiology$gv. 51, n. 3, p. 1447-1451, Sep 2020.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Biblioteca |
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Tipo/Formato |
Classificação |
Cutter |
Registro |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
18/12/2019 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ARAÚJO, R. S.; GARCIA, G. M.; VILELA, J. M. C.; ANDRADE, M. S.; OLIVEIRA, L. A. M.; KANO, E. K.; LANGE, C. C.; BRITO, M. A. V. P. E; BRANDAO, H. de M.; MOSQUEIRA, V. C. F. |
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Afiliação: |
RAQUEL SILVA ARAÚJO; GIANI MARTINS GARCIA; JOSÉ MARIO CARNEIRO VILELA; MARGARETH SPANGLER ANDRADE; LASER ANTÔNIO MACHADO OLIVEIRA; EUNICE KAZUE KANO; CARLA CHRISTINE LANGE, CNPGL; MARIA APARECIDA VASCONCELOS PAIVA E BRITO; HUMBERTO DE MELLO BRANDAO, CNPGL; VANESSA CARLA FURTADO MOSQUEIRA. |
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Título: |
Cloxacillin benzathine-loaded polymeric nanocapsules: Physicochemical characterization, cell uptake, and intramammary antimicrobial effect. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Materials Science & Engineering C, v. 104, 110006, 2019. |
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DOI: |
https://doi.org/10.1016/j.msec.2019.110006 |
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Idioma: |
Inglês |
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Conteúdo: |
The present work shows the development and evaluation of the veterinary antibiotic cloxacillin benzathine(CLOXB) loaded into poly-ε-caprolactone (PCL) nanocapsules (NC), as a potential new treatment strategy tomanage bovine intramammary infections, such as mastitis.Staphylococcus aureus-induced mastitis is often arecurrent disease due to the persistence of bacteria within infected cells. CLOXB-PCL NC were prepared byinterfacial deposition of preformed biodegradable polymer followed by solvent displacement method. The meandiameter of NC varied from 241 to 428 nm and from 326 to 375 nm, when determined by dynamic light scat-tering and by atomic force microscopy, respectively. The zeta potential of NC was negative and varied from−28to−51 mV.In vitrorelease studies from the NC were performed in two media undersinkconditions: PBS with1% polyethylene glycol or milk. A reversed-phase HPLC method was developed to determine the NC entrapmentefficiency and kinetics of CLOXB release from the NC. Free CLOXB dissolution occurred very fast in both media,while drug release from the NC was slower and incomplete (below 50%) after 9 h. CLOXB release kinetics frompolymeric NC wasfitted with the Korsmeyer-Peppas model indicating that CLOXB release is governed by dif-fusion following Fick's law. Thefluorescence confocal microscopy images of macrophage-like J774A.1 cellsreveal NC uptake and internalizationin vitro. In addition, antimicrobial effect of the intramammary adminis-tration of CLOXB-PCL NC in cows with mastitis resulted in no clinical signs of toxicity and allowed completepathogen elimination after treatment. Thein vivoresults obtained in this work suggest that CLOXB-PCL NC couldbe a promising formulation for the treatment of intramammary infections in cattle, considering their physico-chemical properties, release profiles and effects on bovine mastitis control. MenosThe present work shows the development and evaluation of the veterinary antibiotic cloxacillin benzathine(CLOXB) loaded into poly-ε-caprolactone (PCL) nanocapsules (NC), as a potential new treatment strategy tomanage bovine intramammary infections, such as mastitis.Staphylococcus aureus-induced mastitis is often arecurrent disease due to the persistence of bacteria within infected cells. CLOXB-PCL NC were prepared byinterfacial deposition of preformed biodegradable polymer followed by solvent displacement method. The meandiameter of NC varied from 241 to 428 nm and from 326 to 375 nm, when determined by dynamic light scat-tering and by atomic force microscopy, respectively. The zeta potential of NC was negative and varied from−28to−51 mV.In vitrorelease studies from the NC were performed in two media undersinkconditions: PBS with1% polyethylene glycol or milk. A reversed-phase HPLC method was developed to determine the NC entrapmentefficiency and kinetics of CLOXB release from the NC. Free CLOXB dissolution occurred very fast in both media,while drug release from the NC was slower and incomplete (below 50%) after 9 h. CLOXB release kinetics frompolymeric NC wasfitted with the Korsmeyer-Peppas model indicating that CLOXB release is governed by dif-fusion following Fick's law. Thefluorescence confocal microscopy images of macrophage-like J774A.1 cellsreveal NC uptake and internalizationin vitro. In addition, antimicrobial effect of the intramammary adminis-tra... Mostrar Tudo |
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Palavras-Chave: |
Cell uptake; Cloxacillin benzathine; Intramammary; Release kinetics. |
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Thesaurus NAL: |
Mastitis; Nanocapsules. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02882naa a2200313 a 4500
001 2117206
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.msec.2019.110006$2DOI
100 1 $aARAÚJO, R. S.
245 $aCloxacillin benzathine-loaded polymeric nanocapsules$bPhysicochemical characterization, cell uptake, and intramammary antimicrobial effect.$h[electronic resource]
260 $c2019
520 $aThe present work shows the development and evaluation of the veterinary antibiotic cloxacillin benzathine(CLOXB) loaded into poly-ε-caprolactone (PCL) nanocapsules (NC), as a potential new treatment strategy tomanage bovine intramammary infections, such as mastitis.Staphylococcus aureus-induced mastitis is often arecurrent disease due to the persistence of bacteria within infected cells. CLOXB-PCL NC were prepared byinterfacial deposition of preformed biodegradable polymer followed by solvent displacement method. The meandiameter of NC varied from 241 to 428 nm and from 326 to 375 nm, when determined by dynamic light scat-tering and by atomic force microscopy, respectively. The zeta potential of NC was negative and varied from−28to−51 mV.In vitrorelease studies from the NC were performed in two media undersinkconditions: PBS with1% polyethylene glycol or milk. A reversed-phase HPLC method was developed to determine the NC entrapmentefficiency and kinetics of CLOXB release from the NC. Free CLOXB dissolution occurred very fast in both media,while drug release from the NC was slower and incomplete (below 50%) after 9 h. CLOXB release kinetics frompolymeric NC wasfitted with the Korsmeyer-Peppas model indicating that CLOXB release is governed by dif-fusion following Fick's law. Thefluorescence confocal microscopy images of macrophage-like J774A.1 cellsreveal NC uptake and internalizationin vitro. In addition, antimicrobial effect of the intramammary adminis-tration of CLOXB-PCL NC in cows with mastitis resulted in no clinical signs of toxicity and allowed completepathogen elimination after treatment. Thein vivoresults obtained in this work suggest that CLOXB-PCL NC couldbe a promising formulation for the treatment of intramammary infections in cattle, considering their physico-chemical properties, release profiles and effects on bovine mastitis control.
650 $aMastitis
650 $aNanocapsules
653 $aCell uptake
653 $aCloxacillin benzathine
653 $aIntramammary
653 $aRelease kinetics
700 1 $aGARCIA, G. M.
700 1 $aVILELA, J. M. C.
700 1 $aANDRADE, M. S.
700 1 $aOLIVEIRA, L. A. M.
700 1 $aKANO, E. K.
700 1 $aLANGE, C. C.
700 1 $aBRITO, M. A. V. P. E
700 1 $aBRANDAO, H. de M.
700 1 $aMOSQUEIRA, V. C. F.
773 $tMaterials Science & Engineering C$gv. 104, 110006, 2019.
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