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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
Data corrente: |
29/05/2023 |
Data da última atualização: |
23/05/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MOREIRA, V. J. V.; PINHEIRO, D. H.; LOURENCO, I. T.; BASSO, M. F.; LISEI-DE-SA, M. E.; SILVA, M. C. M. da; DANCHIN, E. G. J.; GUIMARAES, P. M.; GRYNBERG, P.; BRASILEIRO, A. C. M.; MACEDO, L. L. P. de; MORGANTE, C. V.; ENGLER, J. de A.; SA, M. F. G. de. |
Afiliação: |
VALDEIR JUNIO VAZ MOREIRA, Biotechnology Center, PPGBCM, UFRGS; DANIELE HELOÍSA PINHEIRO, National Institute of Science and Technology; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; MARCOS FERNANDO BASSO, National Institute of Science and Technology; MARIA E. LISEI-DE-SA, National Institute of Science and Technology; MARIA CRISTINA MATTAR DA SILVA, Cenargen; ETIENNE G. J. DANCHIN, National Institute of Science and Technology; PATRICIA MESSEMBERG GUIMARAES, Cenargen; PRISCILA GRYNBERG, Cenargen; ANA CRISTINA MIRANDA BRASILEIRO, Cenargen; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; JANICE DE ALMEIDA ENGLER, National Institute of Science and Technology; MARIA FATIMA GROSSI DE SA, Cenargen. |
Título: |
In planta RNAi targeting Meloidogyne incognita Minc16803 gene perturbs nematode parasitism and reduces plant susceptibility. |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
Journal of Pest Science, v. 97, p. 411-427, 2024. |
DOI: |
https://doi.org/10.1007/s10340-023-01623-7 |
Idioma: |
Inglês |
Conteúdo: |
Meloidogyne incognita is one of the most important plant-parasitic nematodes (PPNs) causing severe crop losses worldwide. Plants have evolved complex defense mechanisms to respond to PPNs attacks. Conversely, PPNs have evolved infection mechanisms that involve the secretion of effector proteins into host plants to suppress immune responses and facilitate para- sitism. Therefore, effector genes are attractive targets for the genetic improvement of plant resistance to M. incognita. In this study, we functionally characterized the Minc16803 (Minc3s00746g16803) putative effector gene to evaluate its role during plant-nematode interactions. First, we found that the Minc16803 gene is expressed in all nematode life stages and encodes a protein with an N-terminal signal peptide for secretion, a motif characteristic of effector proteins and with the absence of transmembrane domain. In addition, our data demonstrated that transgenic Arabidopsis thaliana lines overexpressing a Minc16803-dsRNA efficiently downregulated the Minc16803 transcripts in infecting nematodes. Furthermore, transgenic lines were significantly less susceptible to M. incognita compared to wild-type control plants. The number of galls per plant was reduced by up to 84%, while the number of egg masses per plant decreased by up to 93.3%. Moreover, galls and feed- ing sites in the roots of transgenic lines were smaller than those in the control plants. Histological analysis revealed giant cells without cytoplasm, disordered neighboring cells, and malformed maturing nematodes in transgenic galls. Curiously, numerous hatching ppJ2 juveniles were often observed near the female body within the transgenic root tissues before egg mass extrusion. All findings strongly suggest that Minc16803 gene is a promising target to engineer agricultural crops for M. incognita resistance through host-induced gene silencing. MenosMeloidogyne incognita is one of the most important plant-parasitic nematodes (PPNs) causing severe crop losses worldwide. Plants have evolved complex defense mechanisms to respond to PPNs attacks. Conversely, PPNs have evolved infection mechanisms that involve the secretion of effector proteins into host plants to suppress immune responses and facilitate para- sitism. Therefore, effector genes are attractive targets for the genetic improvement of plant resistance to M. incognita. In this study, we functionally characterized the Minc16803 (Minc3s00746g16803) putative effector gene to evaluate its role during plant-nematode interactions. First, we found that the Minc16803 gene is expressed in all nematode life stages and encodes a protein with an N-terminal signal peptide for secretion, a motif characteristic of effector proteins and with the absence of transmembrane domain. In addition, our data demonstrated that transgenic Arabidopsis thaliana lines overexpressing a Minc16803-dsRNA efficiently downregulated the Minc16803 transcripts in infecting nematodes. Furthermore, transgenic lines were significantly less susceptible to M. incognita compared to wild-type control plants. The number of galls per plant was reduced by up to 84%, while the number of egg masses per plant decreased by up to 93.3%. Moreover, galls and feed- ing sites in the roots of transgenic lines were smaller than those in the control plants. Histological analysis revealed giant cells without cytoplasm, disor... Mostrar Tudo |
Palavras-Chave: |
DsRNA; Host-induced gene silencing; Interações planta-nematóide; Nematoide das galhas; Plant-nematode interactions; Root-knot nematode. |
Thesagro: |
Meloidogyne Incognita; Nematóide; Parasito de Planta; Planta. |
Thesaurus Nal: |
Nematode biology; Nematode control; Plant parasitic nematodes. |
Categoria do assunto: |
-- O Insetos e Entomologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/264479/1/In-planta-RNAi-targeting-Meloidogyne-incognita-Minc16803-gene-perturbs-nematode-parasitism-and-reduces-plant-susceptibility.pdf
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Marc: |
LEADER 03269naa a2200445 a 4500 001 2154064 005 2024-05-23 008 2024 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s10340-023-01623-7$2DOI 100 1 $aMOREIRA, V. J. V. 245 $aIn planta RNAi targeting Meloidogyne incognita Minc16803 gene perturbs nematode parasitism and reduces plant susceptibility.$h[electronic resource] 260 $c2024 520 $aMeloidogyne incognita is one of the most important plant-parasitic nematodes (PPNs) causing severe crop losses worldwide. Plants have evolved complex defense mechanisms to respond to PPNs attacks. Conversely, PPNs have evolved infection mechanisms that involve the secretion of effector proteins into host plants to suppress immune responses and facilitate para- sitism. Therefore, effector genes are attractive targets for the genetic improvement of plant resistance to M. incognita. In this study, we functionally characterized the Minc16803 (Minc3s00746g16803) putative effector gene to evaluate its role during plant-nematode interactions. First, we found that the Minc16803 gene is expressed in all nematode life stages and encodes a protein with an N-terminal signal peptide for secretion, a motif characteristic of effector proteins and with the absence of transmembrane domain. In addition, our data demonstrated that transgenic Arabidopsis thaliana lines overexpressing a Minc16803-dsRNA efficiently downregulated the Minc16803 transcripts in infecting nematodes. Furthermore, transgenic lines were significantly less susceptible to M. incognita compared to wild-type control plants. The number of galls per plant was reduced by up to 84%, while the number of egg masses per plant decreased by up to 93.3%. Moreover, galls and feed- ing sites in the roots of transgenic lines were smaller than those in the control plants. Histological analysis revealed giant cells without cytoplasm, disordered neighboring cells, and malformed maturing nematodes in transgenic galls. Curiously, numerous hatching ppJ2 juveniles were often observed near the female body within the transgenic root tissues before egg mass extrusion. All findings strongly suggest that Minc16803 gene is a promising target to engineer agricultural crops for M. incognita resistance through host-induced gene silencing. 650 $aNematode biology 650 $aNematode control 650 $aPlant parasitic nematodes 650 $aMeloidogyne Incognita 650 $aNematóide 650 $aParasito de Planta 650 $aPlanta 653 $aDsRNA 653 $aHost-induced gene silencing 653 $aInterações planta-nematóide 653 $aNematoide das galhas 653 $aPlant-nematode interactions 653 $aRoot-knot nematode 700 1 $aPINHEIRO, D. H. 700 1 $aLOURENCO, I. T. 700 1 $aBASSO, M. F. 700 1 $aLISEI-DE-SA, M. E. 700 1 $aSILVA, M. C. M. da 700 1 $aDANCHIN, E. G. J. 700 1 $aGUIMARAES, P. M. 700 1 $aGRYNBERG, P. 700 1 $aBRASILEIRO, A. C. M. 700 1 $aMACEDO, L. L. P. de 700 1 $aMORGANTE, C. V. 700 1 $aENGLER, J. de A. 700 1 $aSA, M. F. G. de 773 $tJournal of Pest Science$gv. 97, p. 411-427, 2024.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
18/03/2015 |
Data da última atualização: |
10/07/2017 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
URCHEI, M. A.; CORRALES, F. M.; CANUTO, J. C.; QUEIROGA, J. L. de; MALAGODI-BRAGA, K. S.; RAMOS FILHO, L. O.; NEVES, M. C.; RAMOS, M. S. T. A. S.; CAMARGO, R. C. R. de; MORICONI, W. |
Afiliação: |
MARIO ARTEMIO URCHEI, CNPMA; FRANCISCO MIGUEL CORRALES, CNPMA; JOAO CARLOS CANUTO, CNPMA; JOEL LEANDRO DE QUEIROGA, CNPMA; KATIA SAMPAIO MALAGOLI BRAGA, CNPMA; LUIZ OCTAVIO RAMOS FILHO, CNPMA; MARCOS CORREA NEVES, CNPMA; MYRIAN SUELY T A DOS SANTOS RAMOS, CNPMA; RICARDO COSTA RODRIGUES DE CAMARGO, CPAMN; WALDEMORE MORICONI, CNPMA. |
Título: |
Construção do conhecimento e de tecnologias agroecológicas com os agricultores familiares do Pontal do Paranapanema. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
In: FÓRUM DE APRESENTAÇÃO DE RESULTADOS DE PESQUISA: AVANÇOS E OPORTUNIDADES, 1., 2014, Campinas. Anais... Jaguariúna: Embrapa Meio Ambiente, 2014. RE025. 6 p. |
Idioma: |
Português |
Conteúdo: |
Resumo: Descreve-se o trabalho conjunto e participativo entre pesquisadores da Embrapa Meio Ambiente, os agricultores familiares e técnicos do Pontal do Paranapanema, envolvendo a construção e troca de conhecimentos, processos, metodologias e tecnologias adaptadas à realidade sociocultural local visando fortalecer a Rede de Agroecologia regional para o avanço e a consolidação de sistemas agrícolas mais integrados, biodiversos e fundamentados na transição agroecológica. |
Palavras-Chave: |
Agroecologia; Conhecimento. |
Thesagro: |
Agricultura familiar; Agricultura orgânica; Tecnologia agrícola. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161562/1/PT020.pdf
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Marc: |
LEADER 01472nam a2200277 a 4500 001 2011730 005 2017-07-10 008 2014 bl uuuu u00u1 u #d 100 1 $aURCHEI, M. A. 245 $aConstrução do conhecimento e de tecnologias agroecológicas com os agricultores familiares do Pontal do Paranapanema.$h[electronic resource] 260 $aIn: FÓRUM DE APRESENTAÇÃO DE RESULTADOS DE PESQUISA: AVANÇOS E OPORTUNIDADES, 1., 2014, Campinas. Anais... Jaguariúna: Embrapa Meio Ambiente, 2014. RE025. 6 p.$c2014 520 $aResumo: Descreve-se o trabalho conjunto e participativo entre pesquisadores da Embrapa Meio Ambiente, os agricultores familiares e técnicos do Pontal do Paranapanema, envolvendo a construção e troca de conhecimentos, processos, metodologias e tecnologias adaptadas à realidade sociocultural local visando fortalecer a Rede de Agroecologia regional para o avanço e a consolidação de sistemas agrícolas mais integrados, biodiversos e fundamentados na transição agroecológica. 650 $aAgricultura familiar 650 $aAgricultura orgânica 650 $aTecnologia agrícola 653 $aAgroecologia 653 $aConhecimento 700 1 $aCORRALES, F. M. 700 1 $aCANUTO, J. C. 700 1 $aQUEIROGA, J. L. de 700 1 $aMALAGODI-BRAGA, K. S. 700 1 $aRAMOS FILHO, L. O. 700 1 $aNEVES, M. C. 700 1 $aRAMOS, M. S. T. A. S. 700 1 $aCAMARGO, R. C. R. de 700 1 $aMORICONI, W.
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