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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
20/01/2006 |
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Data da última atualização: |
30/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BARRETO, M. R.; GUIMARAES, C. T.; TEIXEIRA, F. F.; PAIVA, E.; VALICENTE, F. H. |
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Afiliação: |
CLAUDIA TEIXEIRA GUIMARAES, CNPMS; FLAVIA FRANCA TEIXEIRA, CNPMS; FERNANDO HERCOS VALICENTE, CNPMS. |
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Título: |
Effect of baculovirus spodoptera isolates in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Neotropical Entomology, Londrina, v. 34, n. 1, p. 67-75, 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
The total of 22 Baculovirus isolates surveyed in different corn producing regions in Brazil were used against fall armyworm, Spodoptera frugiperda (J.E. Smith). The viruses were purified and their suspensions were used to feed fall armyworm larvae from 4th and 5th instar. The mortality rate was checked daily and the infected larvae were frost after death, what generally occurred between the 5th and 7th day after virus ingestion. The 22 Baculovirus isolates were used in six concentrations (from 103 to 108 polyhedra/ml) and one check treatment with water. Mortality rate, larval period, pupal period, pupa weight and lethal concentration (LC50) were determined for all isolates. Significant differences were found among all isolates and different concentrations, also interaction between isolate x virus concentration for all characteristics evaluated, except for pupal period. Amplification patterns of 54 RAPD markers, being 41 polymorphic among the isolates, were used to evaluate the genetic distance and its correlation with the fall armyworm larvae mortality rate. The genetic diversity calculated by the Jaccard's coefficient using the molecular data allowed a division of the isolates into two groups, with a high level of confidence. These groups did not present any association with the mortality rate caused by the isolates or with their geographical distribution. However, a RAPD fragment OPW04.2280 was highly associated with the larvae mortality rate and with LC50, explaining 23 and 65% of the phenotypic variation for these traits among the isolates, respectively.. MenosThe total of 22 Baculovirus isolates surveyed in different corn producing regions in Brazil were used against fall armyworm, Spodoptera frugiperda (J.E. Smith). The viruses were purified and their suspensions were used to feed fall armyworm larvae from 4th and 5th instar. The mortality rate was checked daily and the infected larvae were frost after death, what generally occurred between the 5th and 7th day after virus ingestion. The 22 Baculovirus isolates were used in six concentrations (from 103 to 108 polyhedra/ml) and one check treatment with water. Mortality rate, larval period, pupal period, pupa weight and lethal concentration (LC50) were determined for all isolates. Significant differences were found among all isolates and different concentrations, also interaction between isolate x virus concentration for all characteristics evaluated, except for pupal period. Amplification patterns of 54 RAPD markers, being 41 polymorphic among the isolates, were used to evaluate the genetic distance and its correlation with the fall armyworm larvae mortality rate. The genetic diversity calculated by the Jaccard's coefficient using the molecular data allowed a division of the isolates into two groups, with a high level of confidence. These groups did not present any association with the mortality rate caused by the isolates or with their geographical distribution. However, a RAPD fragment OPW04.2280 was highly associated with the larvae mortality rate and with LC50, explaining 23 a... Mostrar Tudo |
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Palavras-Chave: |
Lagarta do cartucho. |
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Thesagro: |
Bactéria; Zea Mays. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/47464/1/Effect-baculovirus.pdf
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Marc: |
LEADER 02254naa a2200205 a 4500
001 1489109
005 2018-05-30
008 2005 bl uuuu u00u1 u #d
100 1 $aBARRETO, M. R.
245 $aEffect of baculovirus spodoptera isolates in Spodoptera frugiperda (J. E. Smith) (Lepidoptera$bNoctuidae) larvae and their characterization by RAPD.$h[electronic resource]
260 $c2005
520 $aThe total of 22 Baculovirus isolates surveyed in different corn producing regions in Brazil were used against fall armyworm, Spodoptera frugiperda (J.E. Smith). The viruses were purified and their suspensions were used to feed fall armyworm larvae from 4th and 5th instar. The mortality rate was checked daily and the infected larvae were frost after death, what generally occurred between the 5th and 7th day after virus ingestion. The 22 Baculovirus isolates were used in six concentrations (from 103 to 108 polyhedra/ml) and one check treatment with water. Mortality rate, larval period, pupal period, pupa weight and lethal concentration (LC50) were determined for all isolates. Significant differences were found among all isolates and different concentrations, also interaction between isolate x virus concentration for all characteristics evaluated, except for pupal period. Amplification patterns of 54 RAPD markers, being 41 polymorphic among the isolates, were used to evaluate the genetic distance and its correlation with the fall armyworm larvae mortality rate. The genetic diversity calculated by the Jaccard's coefficient using the molecular data allowed a division of the isolates into two groups, with a high level of confidence. These groups did not present any association with the mortality rate caused by the isolates or with their geographical distribution. However, a RAPD fragment OPW04.2280 was highly associated with the larvae mortality rate and with LC50, explaining 23 and 65% of the phenotypic variation for these traits among the isolates, respectively..
650 $aBactéria
650 $aZea Mays
653 $aLagarta do cartucho
700 1 $aGUIMARAES, C. T.
700 1 $aTEIXEIRA, F. F.
700 1 $aPAIVA, E.
700 1 $aVALICENTE, F. H.
773 $tNeotropical Entomology, Londrina$gv. 34, n. 1, p. 67-75, 2005.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 387 | |
| 1. |  | GUIMARAES, C. T. Aplicação de marcadores moleculares no melhoramento genético In: CONGRESSO NACIONAL DE MILHO E SORGO, 25.; SIMPOSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 1., 2004, Cuiabá, MT. Da agricultura familiar ao agronegócio: tecnologia, competitividade e sustentabilidade: [palestras]. Sete Lagoas: ABMS: Embrapa Milho e Sorgo; Cuiabá: Empaer, 2004. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
|    |
| 3. | | GUIMARAES, C. T. Genoma milho: o estado da arte. In: CONGRESSO NACIONAL DE MILHO E SORGO, 24., 2002, Florianópolis, SC. Meio ambiente e a nova agenda para o agronegócio de milho e sorgo: [palestras]. Sete Lagoas: ABMS: Embrapa Milho e Sorgo; Florianópolis: Epagri, 2002.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 18. | | CANIATO, F. F.; GUIMARAES, C. T.; SCHAFFERT, R. E.; KOCHIAN, L. V.; MAGALHAES, J. V. D. Association mapping for aluminum tolerance in candidate regions of the Altsb locus in sorghum. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 150.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 19. | | LOGUERCIO, L. L.; SANTOS, C. G.; BARRETO, M. R.; GUIMARAES, C. T.; PAIVA, E. Association of PCR and feeding bioassays as a large-scale method to screen tropical Bacillus thuringiensis isolates for a cry constitution with higher insecticidal effect against Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae. Letters in Applied Microbiology, Oxford, v. 32, n. 5, p. 362-367, 2001.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Milho e Sorgo. |
|  |
| 20. | | PADILHA, L.; LOPES, M. M.; VIEIRA, M. G. G. C.; GUIMARAES, C. T. Análises de microssatélites em bulk e perfis genéticos para híbridos de milho. In: CONGRESSO NACIONAL DE MILHO E SORGO, 25.; SIMPOSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 1., 2004, Cuiabá, MT. Da agricultura familiar ao agronegócio: tecnologia, competitividade e sustentabilidade: [resumos expandidos]. Sete Lagoas: ABMS: Embrapa Milho e Sorgo; Cuiabá: Empaer, 2004. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 387 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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