Data/hora: 15/06/2024 00:16:52

Biblioteca(s): | Embrapa Meio Norte / UEP-Parnaíba.
Data corrente: | 25/01/1996
Data da última atualização: | 25/01/1996
Autoria: | CARLINI, C. R.; GUIMARAES, J. A.
Afiliação: | Universidade Federal Fluminense.
Título: | Isolation and characterization of a toxid protein from Canavalia ensiformis (Jack bean) seeds, distinct from concanavalin A.
Ano de publicação: | 1981
Fonte/Imprenta: | Toxicon, v.19, n.5, p.667-675, 1981.
Idioma: | Inglês
Conteúdo: | A toxic protein present in the crude extract of Canavalia ensiformis seeds induces within 24hr dyspnoea, ataxia, hypothermia, coma, tonic convulsion and death in mice injected i.p. with 100-200mg of protein per kg. The toxing was separated from concanavalin A by affinity adsorption of the latter on sephadex G-100, and further purified by sequentialfractionation by (a) removal of polysaccharide with 30% v/v ethanol; (b) ammonium sulfate precipitation at 0,35-0.55 saturation and (c) DEAE-cellulose chromatography. The purified toxin, within an LD50 of 2-5mg of protein/kg mouse, is unstable. Gel-filtration of the purified toxin on Bio-gel P-200 showed a single protein peak with a molecular weight corresponding to 88,000 daltons. Polyacrylamide gel electrophoresis of this  material indicated the presence of two small contaminants. A central convulsive effect of the toxin can  be proposed since no effects were found on isolated pharmacological preparation. The name CANATOXIN  is suggested for this new toxic protein.
Palavras-Chave: | Proteina toxica; Toxic protein.
Categoria do assunto: | --
Registro original: | Embrapa Meio Norte / UEP-Parnaíba (CPAMN-UEPP)
Exemplares: | CPAMN-UEPP - (4817-1) - ADD - SP - -- -  -  - 1996.00027 - DIS